Acetylcholinesterase/ACHE Assay Kit (Fluorometric)

Acetylcholinesterase/ACHE Assay Kit (Fluorometric) [KA4132] - The standard curve is for the purpose of demonstration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

• Reactivity: Rt
• Applications: Func
• Suitable Sample Type: Biological Samples
• Standard Curve Range: 0.1 to 100 mU/mL
• Sensitivity: 0.1 mU/mL

Acetylcholinesterase/ACHE Assay Kit (Fluorometric) Summary

• Description: Quality control test: Standard curve Acetylcholinesterase Assay Kit (Red Fluorescence) is a one-step fluorometric assay for the detection of AChE activity.
• Specificity: The kit is an optimized 'mix and read' assay that provides a simple one- step fluorimetric assay to detect as little as 0.01 mU AChE in a 100 uL assay volume (0.1 mU/mL). Its signal can be easily read with a fluorescence microplate reader at Ex/Em = ~540/590 nm or an absorbance microplate reader at ~575 nm.
• Standard Curve Range: 0.1 to 100 mU/mL
• Sensitivity: 0.1 mU/mL
• Assay Type: Fluorometric
• Sample Volume: 50 uL
• Kit Type: Assay Kit (Fluorometric)
• Gene: ACHE

Applications/Dilutions

• Dilutions:
  • Functional

Packaging, Storage & Formulations

• Storage: Store at -20C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Acetylcholinesterase/ACHE Assay Kit (Fluorometric)

• acetylcholinesterase (Yt blood group)
• Acetylcholinesterase
• ACHE
• apoptosis-related acetylcholinesterase
• ARACHE
• EC 3.1.1
• EC 3.1.1.7
• N-ACHE
• Yt blood group
• YT

Background

Acetylcholinesterase (AChE) is one of the most crucial enzymes for nerve response and function. AChE degrades the neurotransmitter acetylcholine (ACh) into choline and acetic acid. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate the synaptic transmission. AChE inhibitors are among the key drugs approved for Alzheimer's disease (AD) and myasthenia gravis.Acetylcholinesterase (AChE) is one of the most crucial enzymes for nerve response and function. AChE degrades the neurotransmitter acetylcholine (ACh) into choline and acetic acid. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate the synaptic transmission. AChE inhibitors are among the key drugs approved for Alzheimer's disease (AD) and myasthenia gravis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Acetylcholinesterase/ACHE Kit (KA4132)(3)

Publications using KA4132

• Vang A, da Silva GonCalves Bos D, Fernandez-Nicolas A et al. Alpha 7 nicotinic acetylcholine receptor mediates right ventricular fibrosis and diastolic dysfunction in pulmonary hypertension JCI insight 2021-05-11 [PMID: 33974567]
• Maher A, Saleh S, Elguindy N et al. Exogenous melatonin restrains neuroinflammation in high fat diet induced diabetic rats through attenuating indoleamine 2,3-dioxygenase 1 expression. Life Sci. 2020-02-15 [PMID: 32067945]
• Arafa M, Atteia H Protective Role of Epigallocatechin Gallate in a Rat Model of Cisplatin-Induced Cerebral Inflammation and Oxidative Damage: Impact of Modulating NF-?B and Nrf2. Neurotox Res. 2019-08-13 [PMID: 31410684]

Bioinformatics

• Gene Symbol: ACHE