Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric)

Acetylcholinesterase/ACHE Assay Kit (Colorimetric) [KA3962] - Standard Curve Reference

Product Discontinued

Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric) Summary

• Description: Quality control test: Standard Curve ACHE Activity Assay Kit (Colorimetric) is used for measuring Acetylcholinesterase activity using colorimetric methods.
• Specificity: This assay kit can be used for measurement of AChE activity in various tissues/cells and screening of AChE inhibitors. Suitable sample types include serum, plasma, or blood, animal tissues (liver, hear, kidney, etc), and cell culture (adherent or suspension cells).
• Standard Curve Range: 0.02 umol/ml to 0.1 umol/ml
• Sensitivity: 0.5 mU/mL
• Assay Type: Colorimetric
• Sample Volume: 50 uL
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: ACHE

Applications/Dilutions

• Dilutions:
  • ELISA
  • Functional

Packaging, Storage & Formulations

• Storage: Store at -20C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric)

• acetylcholinesterase (Yt blood group)
• Acetylcholinesterase
• ACHE
• apoptosis-related acetylcholinesterase
• ARACHE
• EC 3.1.1
• EC 3.1.1.7
• N-ACHE
• Yt blood group
• YT

Background

Acetylcholinesterase (AChE) is one of the most crucial enzymes associated with nerve response and function. AChE is a serine protease that hydrolyzes the neurotransmitter acetylcholine. Irreversible inhibition of AChE may lead to muscular paralysis, convulsions, bronchial constriction, and death by asphyxiation. ACHE Activity Assay Kit (Colorimetric) provides a simple and sensitive method for continuous monitoring of enzymatic activity using colorimetry (OD 570 nm). In this assay, AChE converts acetylcholine substrate to choline, which is then oxidized by choline oxidase (CO) to produce an intermediate. The intermediate reacts with a highly specific probe to generate color. This assay kit can detect acetylcholinesterase activity as low as 0.5 mU/ml in a variety of samples.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Acetylcholinesterase/ACHE Kit (KA3962)(1)

Publication using KA3962

• Avci ME, Keskus AG, Targen S et al. Development of a novel zebrafish xenograft model in ache mutants using liver cancer cell lines. Sci Rep 2018-01-25 [PMID: 29371671]

Bioinformatics

• Gene Symbol: ACHE