Acetylcholinesterase/ACHE Antibody (ZR3)

Product Discontinued

Acetylcholinesterase/ACHE Antibody (ZR3) Summary

• Immunogen: Purified rat brain acetylcholinesterase.
• Marker: Early Neuronal Development Marker
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACHE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 3 - 5 ug / 10^6 cells
  • Gel Supershift Assay 1:1 - 1:100
  • Immunocytochemistry/ Immunofluorescence 1:20
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:10 - 1:2000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500 - 1:2000
• Application Notes: Gell Shift assay usage was reported in scientific literature (PMID: 3510009).Does not work in WB.IHC: Staining of Ach in rat brain with this antibody results in staining of nerve fibers and terminals. This antibody reacts with both the soluble and membrane-associated AChE.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1.7 mg/ml
• Purity: Protein A purified

Alternate Names for Acetylcholinesterase/ACHE Antibody (ZR3)

• acetylcholinesterase (Yt blood group)
• Acetylcholinesterase
• ACHE
• apoptosis-related acetylcholinesterase
• ARACHE
• EC 3.1.1
• EC 3.1.1.7
• N-ACHE
• Yt blood group
• YT

Background

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACHE
• Entrez:
  • 43(Human)
• Uniprot:
  • P22303(Human)