Acetyl CoA synthetase Antibody (JF0917)

Immunocytochemistry/Immunofluorescence: Acetyl CoA synthetase Antibody (JF0917) [NBP2-67003] - Staining Acetyl CoA synthetase in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Acetyl CoA synthetase Antibody (JF0917) [NBP2-67003] - Staining Acetyl CoA synthetase in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Acetyl CoA synthetase Antibody (JF0917) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Acetyl CoA synthetase aa 101-145 / 701. (SwissProt: Q9NR19 Human; SwissProt: Q8BK97 Mouse; SwissProt: Q9QXG4 Mouse; SwissProt: D3ZFF9 Rat; SwissProt: D3ZUL6 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACSS2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Acetyl CoA synthetase Antibody (JF0917)

• ACAS2
• AceCS
• acetate thiokinase
• acetate-CoA ligase
• Acetate--CoA ligase
• Acetyl-CoA synthetase
• acetyl-Coenzyme A synthetase 2 (ADP forming)
• acetyl-coenzyme A synthetase, cytoplasmic
• ACSA
• ACSACECS
• Acyl-activating enzyme
• acyl-CoA synthetase short-chain family member 2EC 6.2.1.1
• cytoplasmic acetyl-coenzyme A synthetase
• dJ1161H23.1
• DKFZp762G026
• EC 6.2.1

Background

Acetyl CoA synthetase encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACSS2