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ACAT Antibody

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Product Details

Summary
Reactivity Hu, Mu, HaSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ACAT Antibody Summary

Immunogen
A fusion protein construct that contains the N-terminal region of human ACAT1. [UniProt# P35610]
Localization
Endoplasmic reticulum, membrane
Specificity
This product is specific for protein corresponding to ACAT1 - Sterol O-acyltransferase 1 (SOAT1) Uniprot# P35610, Gene ID# 6646. [Important Note:- ACAT1 is a symbol for both acyl-Coenzyme A: cholesterol acyltransferase 1 (SOAT1/ACAT1) and for acetyl-CoA acetyltransferase 1 (ACAT1/ THIL, Gene ID 38, Uniprot P24752) which often cause confusion].
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SOAT1
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Western Blot 1:400
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 2-10 ug/ml lysate
Application Notes
This ACAT1 antibody is useful for Immunoprecipitation and Western blot. Immunocytochemistry/Immunofluorescence was reported in scientific literature. In Western blot a band is observed at ~46 kDa representing ACAT1 along with a non-specific band at ~30 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
ACAT Lysate (NBP2-64742)
Publications
Read Publications using
NB400-141 in the following applications:

  • ICC/IF
    1 publication
  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Human, Mouse and Hamster.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for ACAT Antibody

  • ACACT
  • ACACT1
  • ACAT1
  • ACAT-1
  • ACATACAT1
  • acyl-Coenzyme A: cholesterol acyltransferase
  • Acyl-coenzyme A:cholesterol acyltransferase 1
  • Cholesterol acyltransferase 1
  • EC 2.3.1.26
  • RP11-215I23.2
  • SOAT1
  • SOAT1/ACAT1
  • STATSOAT
  • sterol O-acyltransferase (acyl-Coenzyme A: cholesterol acyltransferase) 1
  • sterol O-acyltransferase 1

Background

SOAT1 (sterol O-acyltransferase 1), also designated ACAT1, is a homotetrameric enzyme that catalyzes the formation of cholesterol esters from cholesterol and long-chain fatty acyl coenzyme A. The gene encoding human SOAT1 maps to chromosome 1 and is expressed as a protein that localizes to the endoplasmic reticulum (ER) in several tissues, including liver, kidney, adrenal glands and macrophages. SOAT1 is involved in cellular cholesterol homeostasis as well as in foam cell formation and the subsequent progression of atherosclerosis. Several SOAT inhibitors have been developed for the treatment of atherosclerosis. SOAT2 (sterol O-acyltransferase 2), also known as ACAT2 (acyl coenzyme A), participates in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. SOAT2 is an integral membrane protein that localizes to the ER of human intestinal cells. SOAT2 deficiency contributes to severe mental retardation and hypotonus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 units/mL Recombinant Human IFN‑ alpha (Catalog # 11100-1, dark orange filled histogram) for 20 minutes, then stained with Rabbit Anti-Human Phospho‑STAT2 (Y689) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2890) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
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Applications: WB, Simple Western, CyTOF-ready, ICFlow
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Immunocytochemistry/Immunofluorescence: CISH/CIS-1 Antibody [NBP1-30943] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: CISH/CIS-1 Antibody [NBP1-30943] - Paraffin-embedded Lung CA, using antibody at 1:100 dilution.
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
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(4 Publications)
Species: Hu, Mu
Applications: WB
IL-2 [Unconjugated]
202-IL
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1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
(11 Reviews)
(236 Publications)
Species: Hu
gp130/CD130
Antibody
AF-228-NA
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(7 Publications)
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Applications: WB, Neut

Publications for ACAT Antibody (NB400-141)(4)

We have publications tested in 3 confirmed species: Human, Mouse, Hamster.

We have publications tested in 3 applications: ICC/IF, IP, WB.


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(3)
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Product General Protocols

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WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

ACAT Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SOAT1
Entrez
Uniprot