ACAT Antibody

• Reactivity: Hu, Mu, Ha
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ACAT Antibody Summary

• Immunogen: A fusion protein construct that contains the N-terminal region of human ACAT1. [UniProt# P35610]
• Localization: Endoplasmic reticulum, membrane
• Specificity: This product is specific for protein corresponding to ACAT1 - Sterol O-acyltransferase 1 (SOAT1) Uniprot# P35610, Gene ID# 6646. [Important Note:- ACAT1 is a symbol for both acyl-Coenzyme A: cholesterol acyltransferase 1 (SOAT1/ACAT1) and for acetyl-CoA acetyltransferase 1 (ACAT1/ THIL, Gene ID 38, Uniprot P24752) which often cause confusion].
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOAT1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 2-10 ug/ml lysate
  • Western Blot 1:400
• Application Notes: This ACAT1 antibody is useful for Immunoprecipitation and Western blot. Immunocytochemistry/Immunofluorescence was reported in scientific literature. In Western blot a band is observed at ~46 kDa representing ACAT1 along with a non-specific band at ~30 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse and Hamster.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for ACAT Antibody

• ACACT
• ACACT1
• ACAT-1
• ACATACAT1
• acyl-Coenzyme A: cholesterol acyltransferase
• Acyl-coenzyme A:cholesterol acyltransferase 1
• Cholesterol acyltransferase 1
• EC 2.3.1.26
• RP11-215I23.2
• SOAT1
• STATSOAT
• sterol O-acyltransferase (acyl-Coenzyme A: cholesterol acyltransferase) 1
• sterol O-acyltransferase 1

Background

SOAT1 (sterol O-acyltransferase 1), also designated ACAT1, is a homotetrameric enzyme that catalyzes the formation of cholesterol esters from cholesterol and long-chain fatty acyl coenzyme A. The gene encoding human SOAT1 maps to chromosome 1 and is expressed as a protein that localizes to the endoplasmic reticulum (ER) in several tissues, including liver, kidney, adrenal glands and macrophages. SOAT1 is involved in cellular cholesterol homeostasis as well as in foam cell formation and the subsequent progression of atherosclerosis. Several SOAT inhibitors have been developed for the treatment of atherosclerosis. SOAT2 (sterol O-acyltransferase 2), also known as ACAT2 (acyl coenzyme A), participates in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. SOAT2 is an integral membrane protein that localizes to the ER of human intestinal cells. SOAT2 deficiency contributes to severe mental retardation and hypotonus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACAT Antibody (NB400-141)(6)

Publications using NB400-141

• Chen D, Xia S, Zhang R Et Al. Lysine acetylation restricts mutant IDH2 activity to optimize transformation in AML cells Molecular cell Jul 12 2021 [PMID: 34289383]
• Ren M, Xu H, Xia H et al. Simultaneously targeting SOAT1 and CPT1A ameliorates hepatocellular carcinoma by disrupting lipid homeostasis Cell death discovery May 29 2021 [PMID: 34052835] (IHC, Mouse)
• Hai Q, Ritchey B, Robinet P et al. Quantitative Trait Locus Mapping of Macrophage Cholesterol Metabolism and CRISPR/Cas9 Editing Implicate an ACAT1 Truncation as a Causal Modifier Variant Arterioscler. Thromb. Vasc. Biol. 2017 Nov 02 [PMID: 29097366] (WB, Mouse)
• Bhathena J, Martoni C, Kulamarva A et al. Oral Probiotic Microcapsule Formulation Ameliorates Non-Alcoholic Fatty Liver Disease in Bio F1B Golden Syrian Hamsters PLoS One 2013 [PMID: 23554890] (WB, Hamster)
• Song, J, Kost, C K, Jr, Martin, D S. Androgens augment renal vascular responses to ANG II in New Zealand genetically hypertensive rats. Am J Physiol Regul Integr Comp Physiol;290(6):R1608-15. 2006 Jun. [PMID: 16469840]
• Chang, CC et al. Regulation and immunolocalization of acyl-coenzyme A: cholesterol acyltransferase in mammalian cells as studied with specific antibodies. J Biol Chem;270(49):29532-40. 1995 Dec 8. [PMID: 7493995] (WB, IP, ICC/IF, Human)

Bioinformatics

• Gene Symbol: SOAT1
• Entrez:
  • 6646(Human)
  • 20652(Mouse)
• Uniprot:
  • P35610(Human)