ACADSB Antibody - BSA Free

Western Blot: ACADSB Antibody [NBP2-15237] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ACADSB antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: ACADSB Antibody [NBP2-15237] - HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37c for 30 min.Green: SBCAD protein stained by SBCAD antibody diluted at 1:5000.Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: ACADSB Antibody [NBP2-15237] - Paraffin-embedded Hepatoma, using antibody at 1:500 dilution.

Western Blot: ACADSB Antibody [NBP2-15237] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE; antibody diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: ACADSB Antibody [NBP2-15237] - Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: SBCAD protein stained by SBCAD antibody diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar = 10 um.

• Reactivity: Hu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ACADSB Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ACADSB. The exact sequence is proprietary.
• Localization: Mitochondrion matrix
• Predicted Species: Rhesus Macaque (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACADSB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:5000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ACADSB Antibody - BSA Free

• 2-MEBCAD
• 2-methylbutyryl-coenzyme A dehydrogenase
• ACAD7,2-methylbutyryl-CoA dehydrogenase
• ACADSB
• acyl-CoA dehydrogenase, short/branched chain
• acyl-Coenzyme A dehydrogenase, short/branched chain
• EC 1.3.8.5
• EC 1.3.99,2-methyl branched chain acyl-CoA dehydrogenase
• EC 1.3.99.-
• SBCAD
• SBCADshort/branched chain specific acyl-CoA dehydrogenase, mitochondrial

Background

Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ACADSB
• Uniprot:
  • P45954(Human)