ACADSB Antibody

Images

 
Western Blot: ACADSB Antibody [NBP2-15237] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE; antibody diluted at 1:10000.
Immunohistochemistry-Paraffin: ACADSB Antibody [NBP2-15237] - Paraffin-embedded Hepatoma, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ACADSB Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ACADSB. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACADSB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ACADSB Antibody

  • 2-MEBCAD
  • 2-methylbutyryl-coenzyme A dehydrogenase
  • ACAD7,2-methylbutyryl-CoA dehydrogenase
  • acyl-CoA dehydrogenase, short/branched chain
  • acyl-Coenzyme A dehydrogenase, short/branched chain
  • EC 1.3.99,2-methyl branched chain acyl-CoA dehydrogenase
  • EC 1.3.99.-
  • SBCADshort/branched chain specific acyl-CoA dehydrogenase, mitochondrial

Background

Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACADSB
Uniprot