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ACADM Antibody

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Western Blot: ACADM Antibody [NB100-1121] - Analysis of ACADM in Human Heart lysate (RIPA buffer, 35 ug total protein per lane) using this antibody at 0.05 ug/mL. Primary incubated for 1 hour. Detected by ...read more
Immunohistochemistry-Paraffin: ACADM Antibody [NB100-1121] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: ACADM Antibody [NB100-1121] - Staining of paraffin embedded human liver using this antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ACADM Antibody Summary

Immunogen
Peptide with sequence C-RLIVAREHIDKYKN corresponding to C-Terminus according to NP_000007.1, NP_001120800.1.
Localization
Mitochondrial
Specificity
This antibody is expected to recognize both reported isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACADM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3.75 ug/mL
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.01 - 0.1 ug/mL
Control
ACADM Overexpression Lysate
Human Heart Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using NB100-1121.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ACADM Antibody

  • ACAD1
  • acyl-CoA dehydrogenase, C-4 to C-12 straight chain
  • acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
  • EC 1.3.99
  • EC 1.3.99.3
  • FLJ18227
  • FLJ99884
  • MCADFLJ93013
  • MCADH
  • medium-chain specific acyl-CoA dehydrogenase, mitochondrial

Background

ACADM protein is a medium chain specific (C4 to C12 straight chain) acyl Coenzyme A dehydrogenase. The enzyme catalyzes the initial step of the mitochondrial fatty acid beta oxidation pathway. ACADM expression is induced during periods of fasting, when reliance on fatty acids for energy is increased. Clinical phenotypes are associated with ACADM hereditary deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ACADM Antibody (NB100-1121)(2)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACADM
Entrez
Uniprot