ACAA1 Antibody (CL2662) - Azide and BSA Free

Staining of CACO-2 cells using the Anti-ACAA1 monoclonal antibody) .

Staining of human kidney shows granular cytoplasmic immunoreactivity in renal tubules.

Staining of human endometrium shows granular cytoplasmic immunoreactivity in glandular cells.

Staining of human skeletal muscle shows granular cytoplasmic positivity.

Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Staining of human prostate shows granular cytoplasmic immunoreactivity in glandular cells.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACAA1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL2662
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ACAA1 Antibody (CL2662) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ACAA1 Antibody (CL2662) - Azide and BSA Free (NBP2-42381) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P09110, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACAA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for ACAA1 Antibody (CL2662) - Azide and BSA Free

• acetyl-CoA acyltransferase 1
• Acetyl-CoA acyltransferase
• EC 2.3.1
• peroxisomal

Background

Acetyl-Coenzyme A acyltransferase (ACAA1) is an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACAA1