ACAA1 Antibody (CL2660) - BSA Free

Western Blot: ACAA1 Antibody (CL2660) [NBP2-42383] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line U-251

Immunocytochemistry/Immunofluorescence: ACAA1 Antibody (CL2660) [NBP2-42383] - Staining of CACO-2 cells using the Anti-ACAA1 monoclonal antibody, showing specific staining in peroxisomes in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383] - Staining of human skeletal muscle shows granular cytoplasmic immunoreactivity.

Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383] - Staining of human kidney shows granular cytoplasmic immunoreactivity in renal tubules.

Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383] - Staining of human prostate shows granular cytoplasmic immunoreactivity in glandular cells.

Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clone: CL2660
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

ACAA1 Antibody (CL2660) - BSA Free Summary

• Description: Novus Biologicals Mouse ACAA1 Antibody (CL2660) - BSA Free (NBP2-42383) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P09110, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACAA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (88%), Rat (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for ACAA1 Antibody (CL2660) - BSA Free

• acetyl-CoA acyltransferase 1
• Acetyl-CoA acyltransferase
• EC 2.3.1
• peroxisomal

Background

Acetyl-Coenzyme A acyltransferase (ACAA1) is an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACAA1
• Uniprot:
  • P09110(Human)