ABHD2 Antibody

Western Blot: ABHD2 Antibody [NBP1-82850] - Analysis in control (vector only transfected HEK293T lysate) and ABHD2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402075).

Immunocytochemistry/Immunofluorescence: ABHD2 Antibody [NBP1-82850] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: ABHD2 Antibody [NBP1-82850] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Product Discontinued

ABHD2 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TALYGKMGRVRSPHPYGHRKFITMSDGATSTFDLFEPLAEHCVGDDITMVICPGIANHSEKQYIRTFVDYAQKNGYRCAVLNHLGALPNIELTSPRMFTYGCTWEFGAMVNYIKKTYPLTQLVVVGFSLGGNIVCKYLGETQANQEKVL
• Predicted Species: Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABHD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ABHD2 Antibody

• abhydrolase domain containing 2
• abhydrolase domain-containing protein 2
• alpha/beta hydrolase domain containing protein 2
• EC 3.1.1
• EC 3.1.1.-
• HS1-2
• LABH2PHPS1-2
• Lung alpha/beta hydrolase 2
• MGC111112
• MGC26249
• Protein PHPS1-2

Background

The ABHD2 gene encodes a protein containing an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. The function of this protein has not been determined. Alternative splicing of this gene results in two transcript varia

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABHD2
• Entrez:
  • 11057(Human)
• Uniprot:
  • P08910(Human)