ABCG2/CD338 Overexpression Lysate

Product Discontinued

ABCG2/CD338 Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC205640 and based on accession number NM_004827. The protein contains a C-terminal DDK tag.
• Epitope: C-terminal Myc/DDK
• Specificity: Homo sapiens ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), mRNA.
• Gene: ABCG2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 72.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ABCG2/CD338 Overexpression Lysate

• ABC transporter
• ABC15
• ABCG2
• ABCP
• ABCPMGC102821
• ATP-binding cassette sub-family G member 2
• ATP-binding cassette transporter G2
• ATP-binding cassette, sub-family G (WHITE), member 2
• BCRP
• BCRP1
• BCRPMRX
• BMDP
• Breast cancer resistance protein
• CD338 antigen
• CD338
• CDw338
• EST157481
• mitoxantrone resistance protein
• Mitoxantrone resistance-associated protein
• MXR
• MXRMXR1
• placenta specific MDR protein
• Placenta-specific ATP-binding cassette transporter

Background

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for ABCG2/CD338 Lysate (NBL1-07187). (Showing 1 - 1 of 1 FAQ).

1. Recently I bought your ABCG2 lysate (catalog number: NBL1-07187). I intend to use the lysate for immunoprecipitation with anti-FLAG antibody coupled to agarose beads. The agarose beads however cannot be used in the presence of detergents like SDS, 2-mercaptoethanol, DTT, deoxycholate or guanidine HCl. Could you please provide me the detailed composition of the RIPA buffer used to prepare the lysate?
   • The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).

Bioinformatics

• Gene Symbol: ABCG2