ABCG2/CD338 Antibody (BXP-9)

Product Discontinued

ABCG2/CD338 Antibody (BXP-9) Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 221-394 of mouse BCRP/ABCG2 fused to maltose binding protein.
• Localization: Plasma membrane, integral membrane protein
• Marker: Stem Cell Marker
• Specificity: BXP-9 reacts with an internal epitope of bcrp
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: ABCG2
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:20-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20
  • Western Blot 1:20-1:50
• Theoretical MW: 0.25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.7% BSA
• Preservative: 0.1% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for ABCG2/CD338 Antibody (BXP-9)

• ABC transporter
• ABC15
• ABCG2
• ABCP
• ABCPMGC102821
• ATP-binding cassette sub-family G member 2
• ATP-binding cassette transporter G2
• ATP-binding cassette, sub-family G (WHITE), member 2
• BCRP
• BCRP1
• BCRPMRX
• BMDP
• Breast cancer resistance protein
• CD338 antigen
• CD338
• CDw338
• EST157481
• mitoxantrone resistance protein
• Mitoxantrone resistance-associated protein
• MXR
• MXRMXR1
• placenta specific MDR protein
• Placenta-specific ATP-binding cassette transporter

Background

An ABC transporter. Allows efflux of Hoechst dye, a property that has been used to separate bone marrow side population cells, which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cell line. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABCG2
• Entrez:
  • 26357(Mouse)
• Uniprot:
  • Q7TMS5(Mouse)