ABCB5 Antibody

Western Blot: ABCB5 Antibody [NB100-93365] - (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Detected by chemiluminescence.

Flow Cytometry: ABCB5 Antibody [NB100-93365] - analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ABCB5 Antibody Summary

• Description: Novus Biologicals Goat ABCB5 Antibody (NB100-93365) is a polyclonal antibody validated for use in WB, ELISA and Flow. Anti-ABCB5 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QTQHRNTSKKAQ corresponding to internal region according to NP_848654.3, NP_001157413.1.
• Epitope: C-QTQHRNTSKKAQ
• Specificity: This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ABCB5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: FLOW successful usage on Hep3B cells is reported in scientific literature (PMID: 20682318) and tested in HepG2 Cells
  WB: Approx. 80 kDa band observed in lysates of A431(calculated MW of 89.8 kDa band according to NP_848654.3). Primary incubation was 1 hour.
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCB5 Antibody

• ABCB5 P-gp
• ABCB5
• ABCB5alpha
• ABCB5beta
• ATP-binding cassette protein
• ATP-binding cassette sub-family B member 5
• ATP-binding cassette, sub-family B (MDR/TAP), member 5
• EC 3.6.3
• EC 3.6.3.44
• EST422562
• P-glycoprotein ABCB5

Background

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCB5 Antibody (NB100-93365)(2)

Publications using NB100-93365

• Cheung ST, Cheung PF, Cheng CK et al. Granulin-epithelin precursor and ATP-dependent binding cassette (ABC)B5 regulate liver cancer cell chemoresistance. Gastroenterology 2011-01-01 [PMID: 20682318]
• Frank NY, Margaryan A, Huang Y et al. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res 2005-05-15 [PMID: 15899824]

Bioinformatics

• Gene Symbol: ABCB5
• Entrez:
  • 340273(Human)
• Uniprot:
  • Q2M3G0()