ABAT Overexpression Lysate (Native)

Images

 
Western Blot: ABAT Overexpression Lysate (Adult Normal) [NBL1-07170] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ABAT.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ABAT Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC218980 and based on accession number NM_020686. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens 4-aminobutyrate aminotransferase (ABAT), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA.
Gene
ABAT

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
53.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ABAT

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ABAT Overexpression Lysate (Native)

  • (S)-3-amino-2-methylpropionate transaminase
  • 4-aminobutyrate aminotransferase
  • 4-aminobutyrate aminotransferase, mitochondrial
  • 4-aminobutyrate transaminase
  • EC 2.6.1.19
  • EC 2.6.1.22
  • FLJ17813
  • FLJ30272
  • G
  • GABA aminotransferase
  • GABA transaminase
  • GABA transferase
  • GABA-AT
  • GABA-T
  • GABATNPD009
  • Gamma-amino-N-butyrate transaminase
  • hCG1984265
  • L-AIBAT

Background

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) has a more distal alternate 5' UTR. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for ABAT Lysate (NBL1-07170) (0)

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Bioinformatics

Gene Symbol ABAT