ABAT Antibody

Western Blot: ABAT Antibody [H00000018-D01P] - Analysis of ABAT expression in transfected 293T cell line by ABAT polyclonal antibody.Lane 1: ABAT transfected lysate(56.40 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

ABAT Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: ABAT (NP_000654.2, 1 a.a. - 500 a.a.) full-length human protein. MASMLLAQRLACSFQHSYRLLVPGSRHISQAAAKVDVEFDYDGPLMKTEVPGPRSQELMKQLNIIQNAEAVHFFCNYEESRGNYLVDVDGNRMLDLYSQISSVPIGYSHPALLKLIQQPQNASMFVNRPALGILPPENFVEKLRQSLLSVAPKGMSQLITMACGSCSNENALKTIFMWYRSKERGQRGFSQEELETCMINQAPGCPDYSILSFMGAFHGRTMGCLATTHSKAIHKIDIPSFDWPIAPFPRLKYPLEEFVKENQQEEARCLEEVEDLIVKYRKKKKTVAGIIVEPIQSEGGDNHASDDFFRKLRDIARKHGCAFLVDEVQTGGGCTGKFWAHEHWGLDDPADVMTFSKKMMTGGFFHKEEFRPNAPYRIFNTWLGDPSKNLLLAEVINIIKREDLLNNAAHAGKALLTGLLDLQARYPQFISRVRGRGTFCSFDTPDDSIRNKLILIARNKGVVLGGCGDKSIRFRPTLVFRDHHAHLFLNIFSDILADFK
• Specificity: Reacts with 4-aminobutyrate aminotransferase.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABAT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This antibody is reactive against transfected lysate in WB and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ABAT Antibody

• (S)-3-amino-2-methylpropionate transaminase
• 4-aminobutyrate aminotransferase
• 4-aminobutyrate aminotransferase, mitochondrial
• 4-aminobutyrate transaminase
• ABAT
• EC 2.6.1.19
• EC 2.6.1.22
• FLJ17813
• FLJ30272
• G
• GABA aminotransferase
• GABA transaminase
• GABA transferase
• GABA-AT
• GABAT
• GABA-T
• GABATNPD009
• Gamma-amino-N-butyrate transaminase
• hCG1984265
• L-AIBAT

Background

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABAT Antibody (H00000018-D01P)(2)

Publications using H00000018-D01P

• Schwab C, Yu S, Wong W et al. GAD65, GAD67, and GABAT Immunostaining in Human Brain and Apparent GAD65 Loss in Alzheimer's Disease J Alzheimers Dis 2012-10-31 [PMID: 23114513]
• Lee M, Schwab C, McGeer PL. Astrocytes are GABAergic cells that modulate microglial activity. Glia 2011-01-01 [PMID: 21046567]

Bioinformatics

• Gene Symbol: ABAT
• Entrez:
  • 18(Human)
• Uniprot:
  • NP_000654.2(Human)