A33/GPA33 Antibody

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

A33/GPA33 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse A33
  Leu22-Ile235
  Accession # Q9JKA5
• Specificity: Detects mouse A33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human A33 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPA33
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for A33/GPA33 Antibody

• A33
• cell surface A33 antigen
• glycoprotein A33 (transmembrane)
• Glycoprotein A33
• GPA33
• MGC129986
• MGC129987
• transmembrane glycoprotein A33

Background

Mouse A33, also known as Gpa33, is a 48 kDa type I transmembrane protein belonging to the CTX (cortical thymocyte marker in Xenopus) family of cell-adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular region of A33 contains a V-type and a C2-type Ig-like domain. A33 is believed to be involved in cell-cell adhesion, either between epithelium or between epithelium and T cells. The extracellular domain of mouse A33 shares 87% and 71% amino acid sequence identity with the extracellular region of rat and human A33, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for A33/GPA33 Antibody (AF2756)(1)

Publication using AF2756

• Pekka Kujala, Claudine R. Raymond, Martijn Romeijn, Susan F. Godsave, Sander I. van Kasteren, Holger Wille et al. Prion Uptake in the Gut: Identification of the First Uptake and Replication Sites PLoS Pathogens 2011-12-22 [PMID: 22216002]

Bioinformatics

• Gene Symbol: GPA33
• Uniprot:
  • Q9JKA5()