A2BP1 Antibody (D8H8) [FITC]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clone
D8H8
Clonality
Monoclonal
Host
Mouse

A2BP1 Antibody (D8H8) [FITC] Summary

Immunogen
Mouse recombinant A2BP1 [Swiss-Prot# Q9JJ43]
Localization
Nucleus. Cytoplasm. Note: Predominantly nuclear.
Specificity
Does not cross react with paralogue FOX2
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RBFOX1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
FITC
Format
FITC (A=495, E=519)
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:100
Application Notes
This A2BP1 Antibody (D8H8) is useful for Immunocytochemistry/Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry on paraffin embedded sections. In Western Blot, a band is seen 50-55 kDa representing A2BP1. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human and mouse.

Alternate Names for A2BP1 Antibody (D8H8) [FITC]

  • 2BP1
  • A2BP
  • A2BP1 Hexaribonucleotide-binding protein 1
  • Ataxin-2-binding protein 1
  • FOX1 hexaribonucleotide binding protein 1
  • Fox-1 homolog A
  • FOX-1
  • HRNBP1 ataxin 2-binding protein 1
  • RNA binding protein fox-1 homolog 1
  • RNA binding protein, fox-1 homolog (C. elegans) 1

Background

A2BP1, also known as Ataxin-2-binding protein 1, Fox-1 homolog A and Rbfox1, is a member of the Fox-1 family, which is made up of RNA-binding proteins. A2BP1 regulates tissue-specific alternative splicing during erythropoiesis by locating the UGCAUG sequence and binding to the 5'-splice site while also preventing U2AF2 from binding to the 3' splicing site. A2BP1 may play a role in restricting the pathology of spinocerebellar ataxia type 2 (SCA2). The SCA2 gene produces ataxin-2 proteins which is a cause of familial neurodegenerative diseases. A2BP1 is primarily found in the nucleus but also seen in the cytoplasm and is expressed in the brain, heart, neurons, skeletal muscle, and embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP2-13169APC
Biotin Labeled NBP2-13169B
DyLight 350 Labeled NBP2-13169UV
DyLight 405 Labeled NBP2-13169V
DyLight 405LS Labeled NBP2-13169V3
DyLight 488 Labeled NBP2-13169G
DyLight 550 Labeled NBP2-13169R
DyLight 650 Labeled NBP2-13169C
DyLight 680 Labeled NBP2-13169FR
DyLight 755 Labeled NBP2-13169IR
HRP Labeled NBP2-13169H
PE Labeled NBP2-13169PE
PerCP Labeled NBP2-13169PCP

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Blogs on A2BP1

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol RBFOX1

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