53BP2 Antibody

Western Blot: 53BP2 Antibody [NB100-2859] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-2859 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B). ASPP2 was also immunoprecipitated by rabbit anti- ASPP2 antibody NB100-2858, which recognizes an upstream epitope.

Immunohistochemistry-Paraffin: 53BP2 Antibody [NB100-2859] - Sample: FFPE section of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-ASPP2 used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemist

Product Discontinued

53BP2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 825 and 875 of human Apoptosis-Stimulating Protein of p53, 2 (53BP2) using the numbering given in entry NP_001026855.1 (GeneID 7159).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP53BP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 53BP2 Antibody

• apoptosis-stimulating protein of p53, 2
• ASPP2P53BP2,53BP2apoptosis-stimulating of p53 protein 2
• Bbp
• Bcl2-binding protein
• p53-binding protein 2
• p53BP2
• PPP1R13A
• Renal carcinoma antigen NY-REN-51
• tumor protein p53 binding protein, 2
• tumor protein p53-binding protein, 2
• Tumor suppressor p53-binding protein 2

Background

This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. It is localized to the perinuclear region of the cytoplasm, and regulates apoptosis and cell growth through interactions with other regulatory molecules including members of the p53 family. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP53BP2
• Entrez:
  • 7159(Human)
• Uniprot:
  • Q13625(Human)