5-Lipoxygenase Antibody

Western Blot: 5-Lipoxygenase Antibody [NB100-53782] - (0.3ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Rt, Mu, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

5-Lipoxygenase Antibody Summary

• Immunogen: Peptide with sequence C-ERNKKKQLPYYYLSPD corresponding to C-Terminus according to NP_000689.1.
• Predicted Species: Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ALOX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 85 kDa band observed in human bone marrow and rat stomach lysates (calculated MW of 77.7.0 kDa band according to rat NP_036954).
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  5-Lipoxygenase Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 5-Lipoxygenase Antibody

• 5Lipoxygenase
• 5-Lipoxygenase
• 5-LO
• 5-LOX
• 5LPG
• ALOX5
• arachidonate 5-lipoxygenase
• arachidonic 5-lipoxygenase alpha-10 isoform
• arachidonic 5-lipoxygenase delta-10-13 isoform
• arachidonic 5-lipoxygenase delta-13 isoform
• arachidonic 5-lipoxygenase delta-p10 isoform
• arachidonic acid 5-lipoxygenase
• EC 1.13.11
• EC 1.13.11.34
• leukotriene A4 synthase
• LOG5
• LOX5A
• MGC163204

Background

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-Lipoxygenase Antibody (NB100-53782)(2)

Publications using NB100-53782

• Dulin J N, Moore M L et al. The dual cyclooxygenase/5-lipoxygenase inhibitor licofelone attenuates p-glycoprotein-mediated drug resistance in the injured spinal cord. J Neurotrauma 2013-01-02 [PMID: 22947335] (IF/IHC, Rat)
• Kalayci O, Birben E, Sackesen C et al. ALOX5 promoter genotype, asthma severity and LTC production by eosinophils. Allergy 2006-01-01 [PMID: 16364163]

Bioinformatics

• Gene Symbol: ALOX5
• Entrez:
  • 240(Human)
  • 11689(Mouse)
  • 25290(Rat)
• Uniprot:
  • P09917(Human)