5-Lipoxygenase Antibody (OTI3F1)

Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.(1:2000)

Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Adenocarcinoma of Human ovary tissue using antiALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human colon tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150).

Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human Kidney tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)

Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Carcinoma of Human kidney tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min (1:150)

Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human liver tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150).

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI3F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

5-Lipoxygenase Antibody (OTI3F1) Summary

• Description: Novus Biologicals Mouse 5-Lipoxygenase Antibody (OTI3F1) (NBP2-46513) is a monoclonal antibody validated for use in IHC and WB. Anti-5-Lipoxygenase Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 18-364 of human ALOX5(NP_000689) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALOX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 77.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Mouse reported in scientific (publication PMID: 32544870). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 5-Lipoxygenase Antibody (OTI3F1)

• 5Lipoxygenase
• 5-Lipoxygenase
• 5-LO
• 5-LOX
• 5LPG
• ALOX5
• arachidonate 5-lipoxygenase
• arachidonic 5-lipoxygenase alpha-10 isoform
• arachidonic 5-lipoxygenase delta-10-13 isoform
• arachidonic 5-lipoxygenase delta-13 isoform
• arachidonic 5-lipoxygenase delta-p10 isoform
• arachidonic acid 5-lipoxygenase
• EC 1.13.11
• EC 1.13.11.34
• leukotriene A4 synthase
• LOG5
• LOX5A
• MGC163204

Background

5 Lipoxygenase encodes a protein which, with Arachidonate 5-lipoxygenase-activating protein, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-Lipoxygenase Antibody (NBP2-46513)(1)

Publication using NBP2-46513

• Hijioka M, Futokoro R, Ohto-Nakanishi T et al. Microglia-released leukotriene B4 promotes neutrophil infiltration and microglial activation following intracerebral hemorrhage Int. Immunopharmacol. 2020-06-13 [PMID: 32544870] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: ALOX5