5-Lipoxygenase Antibody (OTI3F1)

Images

 
Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Adenocarcinoma of Human ovary tissue using antiALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in ...read more
Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human colon tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris ...read more
Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human Kidney tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris ...read more
Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Carcinoma of Human kidney tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in ...read more
Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human liver tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clone
OTI3F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

5-Lipoxygenase Antibody (OTI3F1) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 18-364 of human ALOX5(NP_000689) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ALOX5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
77.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-46513 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Use in Mouse reported in scientific (publication PMID: 32544870). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 5-Lipoxygenase Antibody (OTI3F1)

  • 5Lipoxygenase
  • 5-Lipoxygenase
  • 5-LO
  • 5-LOX
  • 5LPG
  • ALOX5
  • arachidonate 5-lipoxygenase
  • arachidonic 5-lipoxygenase alpha-10 isoform
  • arachidonic 5-lipoxygenase delta-10-13 isoform
  • arachidonic 5-lipoxygenase delta-13 isoform
  • arachidonic 5-lipoxygenase delta-p10 isoform
  • arachidonic acid 5-lipoxygenase
  • EC 1.13.11
  • EC 1.13.11.34
  • leukotriene A4 synthase
  • LOG5
  • LOX5A
  • MGC163204

Background

5 Lipoxygenase encodes a protein which, with Arachidonate 5-lipoxygenase-activating protein, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5-Lipoxygenase Antibody (NBP2-46513)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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(1)
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ALOX5