5-HT7 Antibody

Western Blot: 5-HT7 Antibody [NBP1-44051] - (1ug/ml) Analysis of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

5-HT7 Antibody Summary

• Description: Novus Biologicals Goat 5-HT7 Antibody (NBP1-44051) is a polyclonal antibody validated for use in WB and ELISA. Anti-5-HT7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence QNADYCRKKGHDS corresponding to C-Terminus according to NP_000863.1.
• Epitope: C-Terminus
• Specificity: This antibody is expected to recognize isoform a (NP_000863.1) only.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HTR7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in KELLY lysate (calculated MW of 49.5 kDa band according to NP_000863.1). An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 5-HT7 Antibody

• 5HT7
• 5-HT7
• 5-HT-7
• 5-HT75-hydroxytryptamine receptor 7
• 5-HT-X
• 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
• HTR7
• serotonin 5-HT-7 receptor
• Serotonin receptor 7

Background

Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs (

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-HT7 Antibody (NBP1-44051)(1)

Publication using NBP1-44051

• Sjogren B, Svenningsson P. Depletion of the lipid raft constituents, sphingomyelin and ganglioside, decreases serotonin binding at human 5-HT7(a) receptors in HeLa cells. Acta Physiol (Oxf) 2007-05-01 [PMID: 17428232]

Bioinformatics

• Gene Symbol: HTR7
• Entrez:
  • 3363(Human)
• Uniprot:
  • NP_000863.1(Human)