5-HT7 Antibody

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Western Blot: 5-HT7 Antibody [NBP1-44051] - (1ug/ml) Analysis of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

5-HT7 Antibody Summary

Immunogen
Peptide with sequence QNADYCRKKGHDS corresponding to C-Terminus according to NP_000863.1.
Epitope
C-Terminus
Specificity
This antibody is expected to recognize isoform a (NP_000863.1) only.
Clonality
Polyclonal
Host
Goat
Gene
HTR7
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 50 kDa band observed in KELLY lysate (calculated MW of 49.5 kDa band according to NP_000863.1). An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-44051.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 5-HT7 Antibody

  • 5HT7
  • 5-HT7
  • 5-HT-7
  • 5-HT75-hydroxytryptamine receptor 7
  • 5-HT-X
  • 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
  • HTR7
  • serotonin 5-HT-7 receptor
  • Serotonin receptor 7

Background

Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs (

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5-HT7 Antibody (NBP1-44051)(1)

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Bioinformatics

Gene Symbol HTR7
Entrez
Uniprot

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