5-HT2C Antibody

Western Blot: 5-HT2C Antibody [NB100-1524] - (1ug/ml) staining of (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

5-HT2C Antibody Summary

• Immunogen: Peptide with sequence C-QVENLELPVN corresponding to internal region according to NP_000859.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HTR2C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in lysates of human EBV immortalised Lymphoblastoids, T cell MOLT4 and monocytic cell line U937 (calculated MW of 51.8 kDa band according to NP_000859.1).
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 5-HT2C Antibody

• 5-HT1C
• 5-HT-1C
• 5HT2C
• 5-HT2C
• 5-HT-2C
• 5-HT2C5-hydroxytryptamine receptor 1C
• 5-HTR2C
• 5-hydroxytryptamine (serotonin) receptor 2C
• 5-hydroxytryptamine receptor 2C
• HTR1C
• HTR2C
• serotonin 5-HT-2C receptor
• Serotonin Receptor 2C

Background

Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20>m) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-HT2C Antibody (NB100-1524)(1)

Publication using NB100-1524

• Castensson A, Aberg K, McCarthy S, Saetre P, ersson B, Jazin E. Serotonin receptor 2C (HTR2C) schizophrenia: Examination of possible medication genetic influences on expression levels. Am J Med Genet B Neuropsychiatr Genet. 2005-02-16 [PMID: 15717293]

Bioinformatics

• Gene Symbol: HTR2C
• Entrez:
  • 3358(Human)
• Uniprot:
  • P28335()