5-HT1B Antibody (499325)

• Reactivity: Hu
• Applications: IHC
• Clone: 499325
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

5-HT1B Antibody (499325) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human 5HT1B
  Met1-Ser390
  Accession # P28222
• Specificity: Detects human 5HT1B.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HTR1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 5-HT1B Antibody (499325)

• 5HT1B
• 5-HT1B
• 5-HT1B5-HT-1B
• 5HT1DB
• 5-HT1DB
• 5-hydroxytryptamine (serotonin) receptor 1B
• HTR1B
• HTR1D2
• HTR1D2,5-hydroxytryptamine receptor 1B
• HTR1DB
• S12
• S125-HT-1D-beta
• Serotonin 1D beta receptor
• Serotonin receptor 1B

Background

5HT1B is a 7TM receptor for serotonin and is expressed on striatal neurons and Purkinje cells in the cerebellum. 5HT1B ligation inhibits glycinergic signaling, appetite, circadian rhythm light responsiveness, and trigeminal-mediated nociception. It is an important pharmacologic target in the treatment of migrane headaches. Human 5HT1B shares 92% aa sequence identity with mouse and rat 5HT1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-HT1B Antibody (MAB5858)(2)

Publications using MAB5858

• Marloes A. M. Peters, Coby Meijer, Rudolf S. N. Fehrmann, Annemiek M. E. Walenkamp, Ido P. Kema, Elisabeth G. E. de Vries et al. Serotonin and Dopamine Receptor Expression in Solid Tumours Including Rare Cancers Pathology & Oncology Research 2020-07-01 [PMID: 31478179]
• Marloes A. M. Peters, Coby Meijer, Rudolf S. N. Fehrmann, Annemiek M. E. Walenkamp, Ido P. Kema, Elisabeth G. E. de Vries et al. Serotonin and Dopamine Receptor Expression in Solid Tumours Including Rare Cancers Pathology & Oncology Research 2020-07-01 [PMID: 31478179] (Immunohistochemistry, Human)

Bioinformatics

• Gene Symbol: HTR1B
• Uniprot:
  • P28222()