4EBP1 Antibody

Images

 
Western Blot: 4EBP1 Antibody [NBP2-16307] - Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3]. HRP-conjugated ...read more
Immunohistochemistry-Paraffin: eIF4EBP1 Antibody [NBP2-16307] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.
Western Blot: eIF4EBP1 Antibody [NBP2-16307] - Sample (30 ug of whole cell lysate) A: H1299 B: HCT116 15% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: eIF4EBP1 Antibody [NBP2-16307] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Rt, Mu, BvSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.13 mg/ml
     

Genetic Strategies

   

Order Details

4EBP1 Antibody Summary

Immunogen
Full length human 4E-BP1 Recombinant protein.
Predicted Species
Mouse (90%), Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4EBP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.13 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 4EBP1 Antibody

  • 4EBP1
  • 4E-BP1
  • eIF4E-binding protein 1
  • EIF4EBP1
  • eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
  • eukaryotic translation initiation factor 4E-binding protein 1
  • PHAS-I
  • PHAS-IMGC4316
  • Phosphorylated heat- and acid-stable protein regulated by insulin 1

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EIF4EBP1
Entrez
Uniprot