4EBP1 Antibody - BSA Free

Western Blot: 4EBP1 Antibody [NBP1-76621] - eIF [NBP1-76621] - Analysis of eIF4EBP1 in human brain cell lysate with eIF4EBP1 antibody using NBP1-76621 at (A) 2.5, (B) 5 and (C) 10 ug/ml.

Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody [NBP1-76621] - 3T3 cells with eIF4EBP1 antibody at 2 ug/ml.

Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody - BSA Free [NBP1-76621] - Immunofluorescence of 4EBP1 in 3T3 cells with 4EBP1 antibody at 20 ug/ml.Green: 4EBP1 Antibody Blue: DAPI staining

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

4EBP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit 4EBP1 Antibody - BSA Free (NBP1-76621) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1. The immunogen is located within the last 50 amino acids of 4E-BP1. Amino Acid Squence: NSPEDKRAGGEESQ
• Predicted Species: Rat (100%), Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF4EBP1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2-20 ug/ml
  • Western Blot 2.5-10 ug/ml
• Control Peptide:

  4EBP1 Protein (NBP1-76621PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for 4EBP1 Antibody - BSA Free

• 4EBP1
• 4E-BP1
• eIF4E-binding protein 1
• EIF4EBP1
• eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
• eukaryotic translation initiation factor 4E-binding protein 1
• PHAS-I
• PHAS-IMGC4316
• Phosphorylated heat- and acid-stable protein regulated by insulin 1

Background

The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms. One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5'-UTRs. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferationl, at least in part by inhibiting the activity of TOR towards 4E-BP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4EBP1
• Uniprot:
  • NP_004086(Human)