4EBP1 [p Thr46, p Thr37] Antibody (0014) - Azide and BSA Free

Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] - Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with IGF1 (100ng/ml, 15min;+) (line B); treated with IGF1 and -phosphatase (line C) using NBP3-23518 at 1:5000 dilution.

Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] - Western blot analysis of extracts from HEK293, untreated(line A) or treated with IGF1 (100ng/ml, 15min; +)(line B), using NBP3-23518 at 1:5000 dilution (upper) or Mouse Monoclonal 4EBP1 Antibody at 1:5000 dilution (lower).

• Reactivity: Hu
• Applications: WB
• Clone: 0014
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

4EBP1 [p Thr46, p Thr37] Antibody (0014) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to residues around (Thr37, 46) of Human phospho-4EBP1
• Modification: p Thr37, p Thr46
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF4EBP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for 4EBP1 [p Thr46, p Thr37] Antibody (0014) - Azide and BSA Free

• 4EBP1
• 4E-BP1
• eIF4E-binding protein 1
• EIF4EBP1
• eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
• eukaryotic translation initiation factor 4E-binding protein 1
• PHAS-I
• PHAS-IMGC4316
• Phosphorylated heat- and acid-stable protein regulated by insulin 1

Background

4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5' cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4EBP1