14-3-3 sigma/Stratifin Antibody

Images

 
Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - (0.1ug/ml) staining of human skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Analysis in Human Skin shows strong nuclear staining in selected cells of the basal layer.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

14-3-3 sigma/Stratifin Antibody Summary

Immunogen
Peptide with sequence C-DLHTLSEDSYKDST corresponding to internal region according to NP_006133.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SFN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 0.1 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 28 kDa band observed in human skin lysates (calculated MW of 27.8 kDa band according to NP_006133.1). IHC-P: Human skin shows strong nuclear staining in selected cells of the basal layer.
Positive Control
Skin Lysate (NB820-59254)
14-3-3 sigma/Stratifin Lysate (NBL1-15882)
Publications
Read Publication using NB100-2465.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 14-3-3 sigma/Stratifin Antibody

  • 14-3-3 protein sigma
  • 1433 sigma
  • 14-3-3 sigma
  • Epithelial cell marker protein 1
  • HME1
  • SFN
  • stratifin
  • YWHAS

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 14-3-3 sigma/Stratifin Antibody (NB100-2465)(1)


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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol SFN
Entrez
Uniprot