14-3-3 sigma/Stratifin Antibody

Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of Mouse (A) and Rat (B) Skin lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of paraffin embedded human skin. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Image shows strong nuclear staining in selected cells of the basal layer.

Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - (0.1ug/ml) staining of human skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of human skin lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of A431 (A) and U2OS (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

14-3-3 sigma/Stratifin Antibody Summary

• Description: Novus Biologicals Goat 14-3-3 sigma/Stratifin Antibody (NB100-2465) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-14-3-3 sigma/Stratifin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DLHTLSEDSYKDST corresponding to internal region according to NP_006133.1.
• Predicted Species: Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SFN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/mL
  • Immunohistochemistry-Paraffin 2 - 4 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.03 - 0.1 ug/mL
• Application Notes: WB: Approx. 28 kDa band observed in human skin lysates (calculated MW of 27.8 kDa band according to NP_006133.1). IHC-P: Human skin shows strong nuclear staining in selected cells of the basal layer.
• Control:

  Human Skin Whole Tissue Lysate (Adult Whole Normal)
  14-3-3 sigma/Stratifin Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 14-3-3 sigma/Stratifin Antibody

• 14-3-3 protein sigma
• 1433 sigma
• 14-3-3 sigma
• Epithelial cell marker protein 1
• HME1
• SFN
• stratifin
• YWHAS

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 14-3-3 sigma/Stratifin Antibody (NB100-2465)(1)

Publication using NB100-2465

• Lodygin D, Hermeking H. The role of epigenetic inactivation of 14-3-3sigma in human cancer. Cell Res 2005-04-01 [PMID: 15857578]

Bioinformatics

• Gene Symbol: SFN
• Entrez:
  • 2810(Human)
  • 55948(Mouse)
  • 313017(Rat)
• Uniprot:
  • NP_006133.1(Human)