14-3-3 gamma Antibody (6A10) - Azide and BSA Free

Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in NIH/3T3.

Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in PC-12.

Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in IMR-32.

Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in Raw 264.7.

Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10 Analysis of YWHAG expression in K-562.

ELISA: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - Detection limit for recombinant GST tagged YWHAG is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clone: 6A10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

14-3-3 gamma Antibody (6A10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: YWHAG (NP_036611, 70 a.a. ~ 169 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQYESKVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQPTH
• Specificity: YWHAG - tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: YWHAG
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for 14-3-3 gamma Antibody (6A10) - Azide and BSA Free

• 1433 gamma
• 14-3-3 gamma
• 14-3-3 protein gamma
• 14-3-3GAMMA
• KCIP-1
• Protein kinase C inhibitor protein 1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gammapolypeptide
• YWHAG

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAG
• Entrez:
  • 7532(Human)
• OMIM:
  • 605356(Human)
• Uniprot:
  • NP_036611(Human)