14-3-3 eta Antibody

Product Discontinued

14-3-3 eta Antibody Summary

• Immunogen: Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 eta. Peptide sequence: GDREQLLQRARC
• Specificity: Recognizes 14-3-3 eta in all mammals. NB100-65809 may not react with recombinant proteins that are not N-acetylated.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YWHAH
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:3200
  • Immunohistochemistry-Paraffin 1:3200
  • Western Blot 1:3000
• Application Notes: In WB detects a band of approximately 30kDa in HEK293 cell lysates. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with Mouse, Human, Bovine, Chicken, Rabbit, Rat and Sheep. Predicted cross-reactivity based on sequence identity: Mammals.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for 14-3-3 eta Antibody

• 1433 eta
• 14-3-3 eta
• 14-3-3 protein eta
• Protein AS1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide
• YWHA1
• YWHA114-3-3 eta
• YWHAH

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAH
• Entrez:
  • 7533(Human)
• Uniprot:
  • Q04917()