14-3-3 beta/alpha Antibody (9-26)

Western Blot: 14-3-3 beta/alpha Antibody (9-26) [NBP2-67090] - Western blot analysis of 14-3-3 beta/alpha on hybrid fish (crucian-carp) brain tissue lysate using anti-14-3-3 beta/alpha antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (9-26) [NBP2-67090] - Staining of Hela cells using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).

Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (9-26) [NBP2-67090] - Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-14-3-3 beta/alpha antibody. Counter stained with hematoxylin.

Western Blot: 14-3-3 beta/alpha Antibody (9-26) [NBP2-67090] - Analysis on mouse liver lysates using anti-14-3-3b/a Mouse mAb.

Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (9-26) [NBP2-67090] - Analysis of paraffin- embedded human breast carcinoma tissue using anti-14-3-3b/a Mouse mAb.

Product Discontinued

14-3-3 beta/alpha Antibody (9-26) Summary

• Immunogen: Recombinant protein within Human 14-3-3 beta/alpha aa 1-246 / 246. (SwissProt: P31946 Human)
• Localization: Cytoplasm
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: YWHAB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:1000-1:2000

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for 14-3-3 beta/alpha Antibody (9-26)

• 14-3-3 alpha
• 14-3-3 beta
• 14-3-3 protein beta/alpha
• brain protein 14-3-3, beta isoform
• GW128
• HS1
• KCIP-1
• Protein 1054
• Protein kinase C inhibitor protein 1
• protein kinase C inhibitor protein-1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide
• YWHAA

Background

The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints. The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAB