294-HG) induces IL-11 secretion in Saos-2 human osteosarcoma cells. The ED
50 for this effect is equal to less than 4.00 ng/mL." class="big_lightbox">
294-HG) induces IL-11 secretion in Saos-2 human osteosarcoma cells. The ED
50 for this effect is equal to less than 4.00 ng/mL." alt="Recombinant Human HGF (
294-HG) induces IL-11 secretion in Saos-2 human osteosarcoma cells. The ED
50 for this effect is equal to less than 4.00 ng/mL." class="big_thumb" />
354-BP), Recombinant Human EGF (
236-EG), Recombinant Human FGF-10 (
345-FG), Recombinant Human Noggin (
6057-NG), Recombinant Human R-Spondin 1 (
4645-RS), and Recombinant Human Wnt-3a (
5036-WN), along with the other reagents listed in the liver organoid initiation medium recipe in the
human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes Recombinant Human HGF (Catalog # 294-HG), Recombinant Human BMP-7 (
354-BP), Recombinant Human EGF (
236-EG), and Recombinant Human FGF-19 (
969-FG), along with the other reagents listed in the liver organoid differentiation medium recipe in the human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (
MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (
AF2400; red) and counterstained with DAPI (
5748; blue). " class="big_lightbox">
354-BP), Recombinant Human EGF (
236-EG), Recombinant Human FGF-10 (
345-FG), Recombinant Human Noggin (
6057-NG), Recombinant Human R-Spondin 1 (
4645-RS), and Recombinant Human Wnt-3a (
5036-WN), along with the other reagents listed in the liver organoid initiation medium recipe in the
human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes Recombinant Human HGF (Catalog # 294-HG), Recombinant Human BMP-7 (
354-BP), Recombinant Human EGF (
236-EG), and Recombinant Human FGF-19 (
969-FG), along with the other reagents listed in the liver organoid differentiation medium recipe in the human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (
MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (
AF2400; red) and counterstained with DAPI (
5748; blue). " class="big_thumb" />
1324-WN); 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay (blue). The activity is approximately 7-fold greater than the competitor's R-Spondin 1 (red)." alt="Recombinant Human R-Spondin 1 (Catalog # 4645-RS), in the presence of Recombinant Mouse Wnt-3a (Catalog # 1324-WN); 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay (blue). The activity is approximately 7-fold greater than the competitor's R-Spondin 1 (red)." class="big_thumb" />
8420-B3). It has equivalent bioactivity to Sf 21 (baculovirus)-derived Recombinant Human TGF‑ beta 3 (Catalog # 243-B3). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human TGF‑ beta 3 proteins are measured by their ability to inhibit the IL-4-dependent proliferation of Ht-2 mouse T cell. (orange, green, respectively). " alt="As an alternative, please consider our next generation CHO-drived Recombinant Human TGF‑ beta 3 (8420-B3). It has equivalent bioactivity to Sf 21 (baculovirus)-derived Recombinant Human TGF‑ beta 3 (Catalog # 243-B3). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human TGF‑ beta 3 proteins are measured by their ability to inhibit the IL-4-dependent proliferation of Ht-2 mouse T cell. (orange, green, respectively). " class="big_thumb" />
294-HG) induces IL-11 secretion in Saos-2 human osteosarcoma cells. The ED50 for this effect is equal to less than 4.00 ng/mL." alt="Recombinant Human HGF (294-HG) induces IL-11 secretion in Saos-2 human osteosarcoma cells. The ED50 for this effect is equal to less than 4.00 ng/mL." class="big_thumb" />
1324-WN)." alt="Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 8-40 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (
1324-WN). Each trace on the graph represents data obtained from Recombinant Human R-Spondin 1 from a different manufacturing run. The ED50 for this effect is 1.00-10.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a." class="big_thumb" />
236-EG), Recombinant Human Noggin (
236-EG), Recombinant Human Noggin (
354-BP), Recombinant Human EGF (
