MMP-9 Antibodies
We offer MMP-9 Antibodies for use in common research applications: Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Proximity Ligation Assay, Simple Western, Western Blot, ELISA, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence. Each MMP-9 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our MMP-9 Antibodies can be used in a variety of model species: Bovine, Guinea Pig, Human, Mouse, Primate, Rat, Zebrafish. Use the list below to choose the MMP-9 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our MMP-9 polyclonal antibodies and browse our MMP-9 monoclonal antibody catalog.
Alternate Names for MMP-9 Antibodies
anti-MMP-9 antibody, anti-MMP9 antibody, anti-92 kDa gelatinase antibody, anti-92 kDa type IV collagenase antibody, anti-CLG4B antibody, anti-EC 3.4.24 antibody, anti-EC 3.4.24.35 antibody, anti-Gelatinase B antibody, anti-GELB antibody, anti-macrophage gelatinase antibody, anti-MANDP2 antibody, anti-matrix metallopeptidase 9 antibody, anti-matrix metalloproteinase 9 antibody, anti-matrix metalloproteinase-9 antibody, anti-type V collagenase antibody
30 Results for "mmp-9" in Primary Antibodies
![Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of activated MMP9 expression in MMP9 proenzyme incubated with trypsin for various times.](http://images.novusbio.com/fullsize/MMP-9-Antibody-4A3-Western-Blot-NBP2-13173-img0002.jpg "Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of activated MMP9 expression in MMP9 proenzyme incubated with trypsin for various times.")
![Immunohistochemistry: MMP-9 Antibody (4A3) [NBP2-13173] - MMP-9 (green) was detected in murine brain (cortex) using MMP-9-DyLight 488 (4A3) with a concentration of 1:20 in PBS for 2 hours. MMP9-Signals are overlaped with the corresponding phase contrast image. Image from verified customer review. Image using the FITC format of this antibody.](http://images.novusbio.com/fullsize/MMP-9-Antibody-4A3-Immunohistochemistry-NBP2-13173-img0006.jpg)
![Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - Identification of gelatinases by Western blot analysis. A pool of sera from 10 patients with inactive RR-MS was used. For 2-DZ, pooled sera were concentrated 10-fold and 40 ug of total proteins were applied (left panels). For Western blot analysis pooled sera were concentrated 40-fold and 160 ug of total proteins were subjected to 2-DE (right panels). 2-DE gels were cut and the corresponding nitrocellulose membranes were treated with anti-NGAL Ab (A'), anti-MMP-2 Ab (B') and Anti-MMP-9 (4A3) Ab, recognizing the 65 kD and the 82 kD activated forms (C'); Gel C show the region with isoelectric point between pH 4-5.5 and molecular mass between 85 and 55 kD, analysed by Western blot in C', respectively. Image collected and cropped by CiteAb from the following publication (null), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MMP-9-Antibody-4A3-Western-Blot-NBP2-13173-img0011.jpg)
![Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - 1-DZ analysis and identification of gelatinases by Western blot. 1.5 ul of serum from healthy control (HC) or from a patient with inactive RR-MS were loaded on a 7.5% polyacrylamide gel copolymerized with 0.1% (w/v) gelatin (1-DZ). For Western blot analysis, 2 ul of fivefold concentrated (Vivaspin 500, MWCO 50,000; GE Healthcare) serum samples (the same as in 1-DZ) were separated on a 7.5% polyacrylamide gel without gelatin (1-DE), transferred on nitrocellulose membranes and incubated with the following primary antibodies: Anti-NGAL Antibody (5G5) at concentration of 2.0 ug/ml; Anti-MMP-9 (Ab-8) Mouse mAb (IA5) at concentration of 2.66 ug/ml; Anti-MMP-2 (Ab-4) Mouse mAb (75-7F7) at concentration of 1.0 ug/ml and Anti-MMP-9 Ab (4A3) at concentration of 2.0 ug/ml. Image collected and cropped by CiteAb from the following publication (null), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MMP-9-Antibody-4A3-Western-Blot-NBP2-13173-img0012.jpg)
![Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of a FFPE tissue section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled secondary antibody and DAB reagent followed by hematoxylin counterstaining. This antibody generated a specific extracellular and cytoplasmic staining primarily in the cancer cells while the signal was pretty weak in the tumor stroma.](http://images.novusbio.com/fullsize/MMP-9-Antibody-4A3-Immunohistochemistry-Paraffin-NBP2-13173-img0003.jpg)
![Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of a FFPE section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled secondary antibody and DAB reagent followed by hematoxylin counterstaining. This antibody clone generated an extracellular and cytoplasmic staining mainly in the cancer cells while the signal was very weak in the stromal cells of the tumor.](http://images.novusbio.com/fullsize/MMP-9-Antibody-4A3-Immunohistochemistry-Paraffin-NBP2-13173-img0004.jpg)
![Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - 2-DZ analysis & identification of MMP-9 by Western blot. Left panels (A & B): 2-D zymography (2-DZ) of serum samples from healthy control (A) & from an inactive RR-MS patient not subjected to therapy (same patient as in Fig. 2; B). For 2-DZ, aliquots of 35 μl of serum were resuspended in the rehydration solution & subjected to isoelectrofocusing (IEF; 1st dimension) on IPG Dry-Strips of 13 cm in a linear pH gradient of 4–7. After IEF, IPG strips were equilibrated & then applied for the 2nd dimension in a 8.5% (w/v) polyacrylamide gel copolymerized with 0.1% (w/v) gelatin. The isoforms & charge variants of MMP-2 & MMP-9 appear as clear spots of digestion on the dark background of the gel. Right panels (A' & B') represent Western blot analysis of the same sera shown in A & B. Aliquots of 35 μl of serum samples (instead of the usual 20 μl) were subjected to 2D electrophoresis (2-DE) by using 8.5% (w/v) polyacrylamide gels without gelatin. After transfer of the proteins, the nitrocellulose membranes were incubated with an anti MMP-9 (Ab-8) Mouse mAb (IA5) at concentration of 2.66 μg/ml. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24616914), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13173_mouse-monoclonal-mmp-9-antibody-4a3-31020241612243.jpg)
![Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - 2-DZ analysis & identification of MMP-9 by Western blot. Left panels (A & B): 2-D zymography (2-DZ) of serum samples from healthy control (A) & from an inactive RR-MS patient not subjected to therapy (same patient as in Fig. 2; B). For 2-DZ, aliquots of 35 μl of serum were resuspended in the rehydration solution & subjected to isoelectrofocusing (IEF; 1st dimension) on IPG Dry-Strips of 13 cm in a linear pH gradient of 4–7. After IEF, IPG strips were equilibrated & then applied for the 2nd dimension in a 8.5% (w/v) polyacrylamide gel copolymerized with 0.1% (w/v) gelatin. The isoforms & charge variants of MMP-2 & MMP-9 appear as clear spots of digestion on the dark background of the gel. Right panels (A' & B') represent Western blot analysis of the same sera shown in A & B. Aliquots of 35 μl of serum samples (instead of the usual 20 μl) were subjected to 2D electrophoresis (2-DE) by using 8.5% (w/v) polyacrylamide gels without gelatin. After transfer of the proteins, the nitrocellulose membranes were incubated with an anti MMP-9 (Ab-8) Mouse mAb (IA5) at concentration of 2.66 μg/ml. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24616914), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13173_mouse-monoclonal-mmp-9-antibody-4a3-310202416114778.jpg)
![Western Blot: MMP-9 Antibody [NBP1-57940] - Western blot of MMP-9. Image from verified customer review.](http://images.novusbio.com/fullsize/MMP-9-Antibody-Western-Blot-NBP1-57940-img0009.jpg "Western Blot: MMP-9 Antibody [NBP1-57940] - Western blot of MMP-9. Image from verified customer review.")
![Immunohistochemistry-Paraffin: MMP-9 Antibody [NBP1-57940] - Human prostate cancer was detected using HRP/AEC red color stain. Dilution: 2-15 ug/mL.](http://images.novusbio.com/fullsize/MMP-9-Antibody-Immunohistochemistry-Paraffin-NBP1-57940-img0005.jpg)
![Western Blot: MMP-9 Antibody [NBP1-57940] - Analysis of MMP-9 in mouse brain tumor lysate (GL261 implanted mouse glioma tissue, 40 ug/lane) using anti-MMP-9 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/MMP-9-Antibody-Western-Blot-NBP1-57940-img0004.jpg)
![Western Blot: MMP-9 Antibody [NBP1-57940] - HeLa cell lysate. Antibody at 1.25 ug/mL.](http://images.novusbio.com/fullsize/MMP-9-Antibody-Western-Blot-NBP1-57940-img0006.jpg)
![Western Blot: MMP-9 Antibody [NBP1-57940] - Human Fetal Lung. Antibody at 1.0 ug/mL.](http://images.novusbio.com/fullsize/MMP-9-Antibody-Western-Blot-NBP1-57940-img0008.jpg)
![Western Blot: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis using MMP9 mAb against human MMP9 (AA: 238-465) recombinant protein. (Expected MW is 50.6 kDa)](http://images.novusbio.com/fullsize/MMP-9-Antibody-5G3-Western-Blot-NBP2-22181-img0006.jpg "Western Blot: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis using MMP9 mAb against human MMP9 (AA: 238-465) recombinant protein. (Expected MW is 50.6 kDa)")
![Immunocytochemistry/Immunofluorescence: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis of NIH/3T3 cells using MMP9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/MMP-9-Antibody-5G3-Immunocytochemistry-Immunofluorescence-NBP2-22181-img0002.jpg)
![Immunohistochemistry-Paraffin: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis of tissues using MMP9 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MMP-9-Antibody-5G3-Immunohistochemistry-Paraffin-NBP2-22181-img0003.jpg)
![Flow Cytometry: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis of Hela cells using MMP9 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/MMP-9-Antibody-5G3-Flow-Cytometry-NBP2-22181-img0001.jpg)
![Western Blot: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis using MMP9 mAb against HEK293 (1), MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9-hIgGFc transfected HEK293 (3) cell lysate.](http://images.novusbio.com/fullsize/MMP-9-Antibody-5G3-Western-Blot-NBP2-22181-img0005.jpg)
![ELISA: MMP-9 Antibody (5G3) [NBP2-22181] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/MMP-9-Antibody-5G3-ELISA-NBP2-22181-img0004.jpg)
![Western Blot: MMP-9 Antibody (S51-82) [NBP2-59699] - Western Blot analysis of Rat Brain showing detection of ~92 kDa and ~82 kDa (pro and active) MMP-9 protein using Mouse Anti-MMP-9 Monoclonal Antibody, Clone S51-82 (NBP2-59699). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MMP-9 Monoclonal Antibody (NBP2-59699) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~92 kDa and ~82 kDa (pro and active).](http://images.novusbio.com/fullsize/MMP-9-Antibody-S51-82-Western-Blot-NBP2-59699-img0004.jpg "Western Blot: MMP-9 Antibody (S51-82) [NBP2-59699] - Western Blot analysis of Rat Brain showing detection of ~92 kDa and ~82 kDa (pro and active) MMP-9 protein using Mouse Anti-MMP-9 Monoclonal Antibody, Clone S51-82 (NBP2-59699). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MMP-9 Monoclonal Antibody (NBP2-59699) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~92 kDa and ~82 kDa (pro and active).")
![Immunocytochemistry/Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MMP-9 Monoclonal Antibody, Clone S51-82 (NBP2-59699). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-MMP-9 Monoclonal Antibody (NBP2-59699) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) MMP-9 Antibody (D) Composite.](http://images.novusbio.com/fullsize/MMP-9-Antibody-S51-82-Immunocytochemistry-Immunofluorescence-NBP2-59699-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699] - Analysis using Mouse Anti-MMP9 Monoclonal Antibody, Clone S51-82 . Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) MMP9 Antibody (D) Composite.](http://images.novusbio.com/fullsize/MMP-9-Antibody-S51-82-Immunocytochemistry-Immunofluorescence-NBP2-59699-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699] - Identification of MET-like structures within human fetal membrane tissues infected with GBS ex vivo. Fetal membrane tissues were excised from healthy, term placental tissues from women undergoing routine cesarean section. (A and B) Fetal membrane tissues were then infected with GBS on the choriodecidual surface for 48 h prior to fixation and confocal microscopy. Fixed and paraffin-embedded fetal membranes were stained with conjugated primary antibodies against CD163, histone H3, and MMP-9. CD163-positive cells within the membrane tissue are seen extruding contents that stain positive for histones and MMP-9 consistent with METs (white arrowheads). Bars represent 20 um. Image collected and cropped by CiteAb from the following publication (//mbio.asm.org/lookup/doi/10.1128/mBio.02084-18) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MMP-9-Antibody-S51-82-Immunocytochemistry-Immunofluorescence-NBP2-59699-img0003.jpg)
