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Lgr5/GPR49 Antibodies

We offer Lgr5/GPR49 Antibodies for use in common research applications: Agonist Activity, Flow Cytometry, ICC/IF (Negative), Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot. Each Lgr5/GPR49 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Lgr5/GPR49 Antibodies can be used in a variety of model species: Human, Mouse, Porcine. Use the list below to choose the Lgr5/GPR49 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Lgr5/GPR49 polyclonal antibodies and browse our Lgr5/GPR49 monoclonal antibody catalog.

Alternate Names for Lgr5/GPR49 Antibodies

anti-Lgr5/GPR49 antibody, anti-LGR5 antibody, anti-FEX antibody, anti-G protein-coupled receptor 49 antibody, anti-G-protein coupled receptor 49 antibody, anti-G-protein coupled receptor 67 antibody, anti-GPR49G-protein coupled receptor HG38 antibody, anti-GPR67 antibody, anti-GRP49 antibody, anti-HG38 antibody, anti-leucine-rich repeat containing G protein-coupled receptor 5 antibody, anti-leucine-rich repeat-containing G protein-coupled receptor 5 antibody, anti-leucine-rich repeat-containing G-protein coupled receptor 5 antibody, anti-MGC117008 antibody, anti-orphan G protein-coupled receptor HG38 antibody

28 Results for "lgr5-gpr49" in Primary Antibodies

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NBP1-28904

Lgr5/GPR49 Antibody - BSA Free

(1 Review)

(13 Publications)

0.1 ml

NBP1-28904

Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Analysis of human small intestine.

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - BSA Free [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. A, Heat map of 37 genes (shown on the vertical axis), which are 5-fold or greater differentially expressed in ZG & ZF. Genes previously associated with the Wnt signaling pathway are highlighted in red. On the horizontal axis, each pair of ZG & ZF from the 20 adrenals are anonymized & numbered. Probe set & detailed microarray information are available from the National Center for Biotechnology Information Gene Expression Omnibus under accession number GSE64957. B, IHC localization of LGR5 & downstream Wnt signaling proteins in the ZG: from the canonical beta -catenin pathway, beta -catenin & LEF1, & from the noncanonical AP1/JUN pathway, c-JUN & p-c-JUN. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 μm) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Interestingly, beta -catenin staining was mostly membranous or cytoplasmic (rather than nuclear staining as highlighted by the arrows), indicating limited canonical Wnt activation. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients & two from pheochromocytoma patients. Scale bar, 500 μm (low power) & 100 μm (high power). C, capsule. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

AKAP8L controls the chemoresistance in vivo.A 1 × 106 AKAP8L-overexpressing or AKAP8L-knockdown BGC-823/Oxa were implanted in nude mice (tumor development following treatment with Oxa once a week), tumors were dissected and representative images of the tumors are shown. B, C Tumor growth curves for orthotopic models. D Representative images of immunofluorescence staining of AKAP8L, Ki-67 and quantification analysis of AKAP8L, Ki-67 positive cells in xenograft tumors. Scale bar indicates 50 μm. E, F qPCR and Western blot analysis of the expression of AKAP8L, Lgr5, CD133, CD44, Oct4, and Sox2 in xenograft tumors. G Representative immunohistochemical staining of AKAP8L and CD133 in the indicated tumor tissues. Scale bar indicates 20 μm. H Immunofluorescence staining of TUNEL was performed to evaluate the apoptotic cells. Scale bar indicates 50 μm. I Western blot analysis of cleaved-caspase 3 and cleaved-PAPR. The values indicate the mean +/- SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36522343), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The expression levels of CSC markers in HCC cells are decreased by JIB-04. (A) The mRNA expression of cancer stem cell marker genes was analyzed using qRT-PCR in PLC/PRF/5, Huh7, and HepG2 cultures after treatment with DMSO (control), 6 μM JIB-04, or 4 μM TSA (positive control) for 24 h. All data are normalized to GAPDH and plotted relative to the expression level in control cells. Data are represented as mean +/- SEM derived from triplicate measurements (n = 3); * p < 0.05, ** p < 0.01, and *** p < 0.001. (B) (Top panels) Protein levels of CD133, CD44, LGR5, and EpCAM in HCC cells after treatment with 6 μM JIB-04 for 48 h were confirmed with Western blot analysis. GAPDH was used as a loading control. (Bottom panels) Quantification based on densitometry of Western blotting data from top panels in (B). All data are normalized to GAPDH. Data are represented as mean +/- SEM of triplicate measurements; * p < 0.05 and ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35887001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

SCD1 mediates the effects of AKAP8L on GC cell stemness and chemoresistance.SCD1 siRNA was transiently transfected to AKAP8L overexpressing cells. SCD1 inhibitor (CAY10566) was added into AKAP8L overexpressing cells. A Representative images of spheroids in the SCD1 inhibitor, AKAP8L, AKAP8L/SCD1 inhibitor and the control groups. B, C qPCR and Western blot assays showed the stemness-related gene expression induced by AKAP8L/CAY10566 or AKAP8L/si-SCD1. D Cell viability was measured by MTT in four groups after the treatments of Oxa. E Representative images of flow cytometry showed the percentage of apoptotic cells in four groups after the treatments of Oxa. F Western blot analysis of cleaved-caspase 3 and cleaved-PAPR induced by AKAP8L/CAY10566 or AKAP8L/si-SCD1. Scale bar indicates 50 μm. The values indicate the mean +/- SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36522343), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

AKAP8L enhances the stemness in GC cells.A Western blot analysis showed ectopic expression of AKAP8L in GC cells transfected with AKAP8L (AKAP8L) or control lentivirus (Con), and AKAP8L silencing in cells treated with scrambled shRNA (shCon) or shRNA against AKAP8L (shAKAP8L-1, shAKAP8L-2). B Representative images and quantification analysis of spheroids in AKAP8L overexpression or the control groups, and AKAP8L knockdown or the control groups. Scale bar indicates 50 μm. C In soft agar colony formation assay, representative images and numbers of spheroids formed by AKAP8L-overexpressing and AKAP8L-knockdown BGC-823/Oxa and MKN-45/Oxa. Scale bar indicates 50 μm. D, E qPCR and Western blot assays showed the expression of stem cell markers induced by AKAP8L or AKAP8L shRNAs. The values indicate the mean +/- standard deviation (SD) of three independent experiments. *P < 0.05, **P < 0.01,***P < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36522343), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Po

Species Glossary

Reactivity: Hu, Mu, Po

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-28904AF350
Alexa Fluor 405	NBP1-28904AF405
Alexa Fluor 488	NBP1-28904AF488
Alexa Fluor 532	NBP1-28904AF532
Alexa Fluor 594	NBP1-28904AF594
Alexa Fluor 647	NBP1-28904AF647
Alexa Fluor 700	NBP1-28904AF700
Alexa Fluor 750	NBP1-28904AF750
Alexa Fluor Plus 405	NBP1-28904AFP405
Alexa Fluor Plus 488	NBP1-28904AFP488
Alexa Fluor Plus 555	NBP1-28904AFP555
Alexa Fluor Plus 594	NBP1-28904AFP594
Alexa Fluor Plus 647	NBP1-28904AFP647
Alexa Fluor Plus 680	NBP1-28904AFP680
Allophycocyanin	NBP1-28904APC
Biotin	NBP1-28904B
CoraFluor 1	NBP1-28904CL1
DyLight 350	NBP1-28904UV
DyLight 405	NBP1-28904V
DyLight 488	NBP1-28904G
DyLight 550	NBP1-28904R
DyLight 594	NBP1-28904DL594
DyLight 650	NBP1-28904C
DyLight 680	NBP1-28904FR
DyLight 755	NBP1-28904IR
FITC	NBP1-28904F
HRP	NBP1-28904H
Janelia Fluor 525	NBP1-28904JF525
Janelia Fluor 549	NBP1-28904JF549
Janelia Fluor 646	NBP1-28904JF646
Janelia Fluor 669	NBP1-28904JF669
PE	NBP1-28904PE
PerCP	NBP1-28904PCP
mFluor Violet 450 SE	NBP1-28904MFV450
mFluor Violet 500 SE	NBP1-28904MFV500
mFluor Violet 610 SE	NBP1-28904MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-28904SS

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MAB8240

Lgr5/GPR49 Antibody (803420)

(6 Publications)

100 μg

MAB8240

MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_lightbox">

HEK293 human embryonic kidney cell line transfected with mouse Lgr5/GPR49 and eGFP was stained with and either (A) Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240) or (B) Rat IgG<SUB>2B</SUB>Isotype Control (Catalog # <a class=

MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." alt="HEK293 human embryonic kidney cell line transfected with mouse Lgr5/GPR49 and eGFP was stained with and either (A) Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Lgr5/GPR49 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GFP (green) tagged mouse LGR5 using Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

Lgr5/GPR49 was detected in immersion fixed MYC tagged mouse transfectants using Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240). Cells were stained using an anti-Mouse HRP-conjugated secondary antibody (brown).<em>Image courtesy of Dr. Hans Clevers, Hubrecht Institute, The Netherlands.</em>

Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240) induces TOPflash activity in the HEK293 human embryonic kidney cell line stably expressing LGR5 in the presence of Wnt-3a, but in the absence of R-Spondins. (<EM>Data courtesy of Dr. Wim de Lau and Dr. Hans Clevers, Hubrecht Institute, The Netherlands. See Reference 1.</EM>)

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB8240U-100UG
Alexa Fluor 405	FAB8240V-100UG
Alexa Fluor 488	FAB8240G-100UG
Alexa Fluor 532	FAB8240X
Alexa Fluor 594	FAB8240T-100UG
Alexa Fluor 647	FAB8240R-100UG
Alexa Fluor 647	FAB8240R-025UG
Alexa Fluor 700	FAB8240N-100UG
Alexa Fluor 750	FAB8240S-100UG
Alexa Fluor Plus 405	FAB8240AFP405-100UG
Alexa Fluor Plus 488	FAB8240AFP488-100UG
Alexa Fluor Plus 555	FAB8240AFP555-100UG
Alexa Fluor Plus 594	FAB8240AFP594-100UG
Alexa Fluor Plus 647	FAB8240AFP647-100UG
Alexa Fluor Plus 680	FAB8240AFP680-100UG
Allophycocyanin	FAB8240A
Allophycocyanin/Cy7	FAB8240APCCY7
Biotin	FAB8240B
CoraFluor 1	FAB8240CL1
DyLight 350	FAB8240D
DyLight 405	FAB8240E
DyLight 488	FAB8240K
DyLight 550	FAB8240L
DyLight 594	FAB8240M
DyLight 650	FAB8240W
DyLight 680	FAB8240Y
DyLight 755	FAB8240Z
FITC	FAB8240F
HRP	FAB8240H
Janelia Fluor 525	FAB8240JF525
Janelia Fluor 549	FAB8240I
Janelia Fluor 646	FAB8240J
Janelia Fluor 669	FAB8240JF669
PE/Atto594	FAB8240PEATT594
PE/Cy5.5	FAB8240PECY55
PE/Cy7	FAB8240PECY7
PerCP	FAB8240C
Phycoerythrin	FAB8240P-100UG
mFluor Violet 450 SE	FAB8240MFV450
mFluor Violet 500 SE	FAB8240MFV500
mFluor Violet 610 SE	FAB8240MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB8240-SP
Carrier Free	FAB8240R-025UG
Carrier Free	FAB8240R-100UG
Carrier Free	FAB8240V-100UG
Carrier Free	FAB8240U-100UG
Carrier Free	FAB8240T-100UG
Carrier Free	FAB8240S-100UG
Carrier Free	FAB8240N-100UG
Carrier Free	FAB8240G-100UG
Carrier Free	FAB8240P-100UG

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MAB8078

Lgr5/GPR49 Antibody (707042)

(4 Publications)

100 μg

MAB8078

MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_lightbox">

NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with and either (A) Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) or (B) Mouse IgG<SUB>2A</SUB>Isotype Control (Catalog # <a class=

MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with and either (A) Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

Lgr5/GPR49 was detected in immersion fixed NSO mouse myeloma cell line transfected with GFP (green) tagged human LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Lgr5/GPR49 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GFP (green) tagged mouse LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

Lgr5/GPR49 was detected in MYC tagged human transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary antibody (brown). Specific staining was localized to cytoplasm.<em>Image courtesy of Dr. Hans Clevers, Hubrecht Institute, The Netherlands.</em>

Lgr5/GPR49 was detected in immersion fixed MYC tagged mouse transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary antibody (brown). Specific staining was localized to cytoplasm.<em>Image courtesy of Dr. Hans Clevers, Hubrecht Institute, The Netherlands.</em>

Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) induces TOPflash activity in the HEK293 human embryonic kidney cell line stably expressing LGR5 in the presence of Wnt-3a, but in the absence of R-Spondins. (<EM>Data courtesy of Dr. Wim de Lau and Dr. Hans Clevers, Hubrecht Institute, The Netherlands. See Reference 1.</EM>)

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB8078U-100UG
Alexa Fluor 405	FAB8078V-100UG
Alexa Fluor 488	FAB8078G-100UG
Alexa Fluor 532	FAB8078X
Alexa Fluor 594	FAB8078T-100UG
Alexa Fluor 647	FAB8078R-100UG
Alexa Fluor 700	FAB8078N-100UG
Alexa Fluor 750	FAB8078S-100UG
Alexa Fluor Plus 405	FAB8078AFP405-100UG
Alexa Fluor Plus 488	FAB8078AFP488-100UG
Alexa Fluor Plus 555	FAB8078AFP555-100UG
Alexa Fluor Plus 594	FAB8078AFP594-100UG
Alexa Fluor Plus 647	FAB8078AFP647-100UG
Alexa Fluor Plus 680	FAB8078AFP680-100UG
Allophycocyanin	FAB8078A
Allophycocyanin/Cy7	FAB8078APCCY7
Biotin	FAB8078B
CoraFluor 1	FAB8078CL1
DyLight 350	FAB8078D
DyLight 405	FAB8078E
DyLight 488	FAB8078K
DyLight 550	FAB8078L
DyLight 594	FAB8078M
DyLight 650	FAB8078W
DyLight 680	FAB8078Y
DyLight 755	FAB8078Z
FITC	FAB8078F
HRP	FAB8078H
Janelia Fluor 525	FAB8078JF525
Janelia Fluor 549	FAB8078I
Janelia Fluor 646	FAB8078J
Janelia Fluor 669	FAB8078JF669
PE/Atto594	FAB8078PEATT594
PE/Cy5.5	FAB8078PECY55
PE/Cy7	FAB8078PECY7
PerCP	FAB8078C
Phycoerythrin	FAB8078P
mFluor Violet 450 SE	FAB8078MFV450
mFluor Violet 500 SE	FAB8078MFV500
mFluor Violet 610 SE	FAB8078MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB8078P
Carrier Free	FAB8078A
Carrier Free	MAB8078-SP
Carrier Free	FAB8078V-100UG
Carrier Free	FAB8078U-100UG
Carrier Free	FAB8078T-100UG
Carrier Free	FAB8078S-100UG
Carrier Free	FAB8078R-100UG
Carrier Free	FAB8078N-100UG
Carrier Free	FAB8078G-100UG

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NLS1236

Lgr5/GPR49 Antibody - BSA Free

(3 Publications)

0.05 ml

NLS1236

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of anti-GPR49 antibody with human colon.

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of Lgr5/GPR49 with brain, glioblastoma

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of Lgr5/GPR49 with Brain, Caudate

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - BSA Free [NLS1236] - Immunohistochemistry of formalin-fixed, paraffin-embedded Human Brain, Cortex tissue

Reactivity:

Hu, Mu, Rt, Bv, Ha, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ha, Pm

Applications:

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NLS1235

Lgr5/GPR49 Antibody - Azide and BSA Free

0.05 ml

NLS1235

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 405	NLS1235AF405
Alexa Fluor 488	NLS1235AF488
Alexa Fluor 647	NLS1235AF647
Alexa Fluor 700	NLS1235AF700
Biotin	NLS1235B
DyLight 405	NLS1235V
DyLight 488	NLS1235G
DyLight 550	NLS1235R
DyLight 650	NLS1235C
FITC	NLS1235F

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NLS1235SS

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NBP3-24570

Lgr5/GPR49 Antibody (CL13567) - BSA Free

100 ul

NBP3-24570

Staining of human skeletal muscle shows moderate membranous positivity in myocytes.

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Western blot analysis in human cell line SH-SY5Y.

Staining of human ovarian cancer (dysgerminoma) shows strong membranous and cytoplasmic positivity in tumor cells.

Staining of human cerebral cortex shows moderate membranous positivity in a subset of neurons.

Staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.

Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.

Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong cytoplasmic positivity in tumor cells.

<b>Orthogonal Strategies Validation. </b>Analysis in human fallopian tube and tonsil tissues using NBP3-24570 antibody. Corresponding LGR5 RNA-seq data are presented for the same tissues.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43844AF350
Alexa Fluor 405	NBP3-43844AF405
Alexa Fluor 488	NBP3-43844AF488
Alexa Fluor 532	NBP3-43844AF532
Alexa Fluor 594	NBP3-43844AF594
Alexa Fluor 647	NBP3-43844AF647
Alexa Fluor 700	NBP3-43844AF700
Alexa Fluor 750	NBP3-43844AF750
Alexa Fluor Plus 405	NBP3-43844AFP405
Alexa Fluor Plus 488	NBP3-43844AFP488
Alexa Fluor Plus 555	NBP3-43844AFP555
Alexa Fluor Plus 594	NBP3-43844AFP594
Alexa Fluor Plus 647	NBP3-43844AFP647
Alexa Fluor Plus 680	NBP3-43844AFP680
Allophycocyanin	NBP3-43844APC
Biotin	NBP3-43844B
CoraFluor 1	NBP3-43844CL1
DyLight 350	NBP3-43844UV
DyLight 405	NBP3-43844V
DyLight 488	NBP3-43844G
DyLight 550	NBP3-43844R
DyLight 594	NBP3-43844DL594
DyLight 650	NBP3-43844C
DyLight 680	NBP3-43844FR
DyLight 755	NBP3-43844IR
FITC	NBP3-43844F
HRP	NBP3-43844H
Janelia Fluor 525	NBP3-43844JF525
Janelia Fluor 549	NBP3-43844JF549
Janelia Fluor 646	NBP3-43844JF646
Janelia Fluor 669	NBP3-43844JF669
PE	NBP3-43844PE
PerCP	NBP3-43844PCP
mFluor Violet 450 SE	NBP3-43844MFV450
mFluor Violet 500 SE	NBP3-43844MFV500
mFluor Violet 610 SE	NBP3-43844MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-24570-25ul
Azide and BSA Free	NBP3-43844

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NBP3-24571

Lgr5/GPR49 Antibody (CL13579) - BSA Free

100 ul

NBP3-24571

<b>Orthogonal Strategies Validation. </b>Staining of human placenta shows moderate cytoplasmic and membranous positivity in trophoblastic cells.

Staining of human duodenum shows moderate cytoplasmic and membranous positivity in glandular cells.

Staining of human ovarian cancer (endometrioid carcinoma) shows moderate cytoplasmic positivity in tumor cells.

Staining of human tonsil shows mainly very weak positivity in non-germinal center cells.

Staining of human cerebral cortex shows moderate cytoplasmic and membranous positivity in neurons and neuropil.

Staining of human ovarian cancer (papillary carcinoma) shows moderate to strong cytoplasmic and membranous positivity in tumor cells.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43804AF350
Alexa Fluor 405	NBP3-43804AF405
Alexa Fluor 488	NBP3-43804AF488
Alexa Fluor 532	NBP3-43804AF532
Alexa Fluor 594	NBP3-24571AF594
Alexa Fluor 594	NBP3-43804AF594
Alexa Fluor 647	NBP3-43804AF647
Alexa Fluor 700	NBP3-43804AF700
Alexa Fluor 750	NBP3-43804AF750
Alexa Fluor Plus 405	NBP3-43804AFP405
Alexa Fluor Plus 488	NBP3-43804AFP488
Alexa Fluor Plus 555	NBP3-43804AFP555
Alexa Fluor Plus 594	NBP3-43804AFP594
Alexa Fluor Plus 647	NBP3-43804AFP647
Alexa Fluor Plus 680	NBP3-43804AFP680
Allophycocyanin	NBP3-43804APC
Biotin	NBP3-43804B
CoraFluor 1	NBP3-43804CL1
DyLight 350	NBP3-43804UV
DyLight 405	NBP3-43804V
DyLight 488	NBP3-43804G
DyLight 550	NBP3-43804R
DyLight 594	NBP3-43804DL594
DyLight 650	NBP3-43804C
DyLight 680	NBP3-43804FR
DyLight 755	NBP3-43804IR
FITC	NBP3-43804F
HRP	NBP3-43804H
Janelia Fluor 525	NBP3-43804JF525
Janelia Fluor 549	NBP3-43804JF549
Janelia Fluor 646	NBP3-43804JF646
Janelia Fluor 669	NBP3-43804JF669
PE	NBP3-43804PE
PerCP	NBP3-43804PCP
mFluor Violet 450 SE	NBP3-43804MFV450
mFluor Violet 500 SE	NBP3-43804MFV500
mFluor Violet 610 SE	NBP3-43804MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-24571-25ul
Azide and BSA Free	NBP3-43804

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MAB82401

Lgr5/GPR49 Antibody (889901)

100 μg

MAB82401

F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB0061)." class="big_lightbox">

HEK293 human embryonic kidney cell line transfected with (A) mouse Lgr5/GPR49 or (B) irrelevant transfectant and eGFP was stained with Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB82401) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB0061)." alt="HEK293 human embryonic kidney cell line transfected with (A) mouse Lgr5/GPR49 or (B) irrelevant transfectant and eGFP was stained with Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB82401) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB0061)." class="big_thumb" />

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB82401U-100UG
Alexa Fluor 405	FAB82401V-100UG
Alexa Fluor 488	FAB82401G-100UG
Alexa Fluor 532	FAB82401X
Alexa Fluor 594	FAB82401T-100UG
Alexa Fluor 647	FAB82401R-100UG
Alexa Fluor 700	FAB82401N-100UG
Alexa Fluor 750	FAB82401S-100UG
Alexa Fluor Plus 405	FAB82401AFP405-100UG
Alexa Fluor Plus 488	FAB82401AFP488-100UG
Alexa Fluor Plus 555	FAB82401AFP555-100UG
Alexa Fluor Plus 594	FAB82401AFP594-100UG
Alexa Fluor Plus 647	FAB82401AFP647-100UG
Alexa Fluor Plus 680	FAB82401AFP680-100UG
Allophycocyanin	FAB82401A-025UG
Allophycocyanin	FAB82401A-100UG
Allophycocyanin/Cy7	FAB82401APCCY7
Biotin	FAB82401B
CoraFluor 1	FAB82401CL1
DyLight 350	FAB82401D
DyLight 405	FAB82401E
DyLight 488	FAB82401K
DyLight 550	FAB82401L
DyLight 594	FAB82401M
DyLight 650	FAB82401W
DyLight 680	FAB82401Y
DyLight 755	FAB82401Z
FITC	FAB82401F
HRP	FAB82401H
Janelia Fluor 525	FAB82401JF525
Janelia Fluor 549	FAB82401I
Janelia Fluor 646	FAB82401J
Janelia Fluor 669	FAB82401JF669
PE/Atto594	FAB82401PEATT594
PE/Cy5.5	FAB82401PECY55
PE/Cy7	FAB82401PECY7
PerCP	FAB82401C
Phycoerythrin	FAB82401P-100UG
Phycoerythrin	FAB82401P-025UG
mFluor Violet 450 SE	FAB82401MFV450
mFluor Violet 500 SE	FAB82401MFV500
mFluor Violet 610 SE	FAB82401MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB82401-SP
Carrier Free	FAB82401P-025UG
Carrier Free	FAB82401P-100UG
Carrier Free	FAB82401U-100UG
Carrier Free	FAB82401T-100UG
Carrier Free	FAB82401S-100UG
Carrier Free	FAB82401R-100UG
Carrier Free	FAB82401N-100UG
Carrier Free	FAB82401G-100UG
Carrier Free	FAB82401V-100UG
Carrier Free	FAB82401A-100UG
Carrier Free	FAB82401A-025UG

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MAB80781

Lgr5/GPR49 Antibody (750835) [Unconjugated]

100 μg

MAB80781

F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox">

NS0 mouse myeloma cell line transfected with (A) human Lgr5/GPR49 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB80781) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol." alt="NS0 mouse myeloma cell line transfected with (A) human Lgr5/GPR49 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB80781) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB80781U-100UG
Alexa Fluor 405	FAB80781V-100UG
Alexa Fluor 488	FAB80781G-100UG
Alexa Fluor 532	FAB80781X
Alexa Fluor 594	FAB80781T-100UG
Alexa Fluor 647	FAB80781R-100UG
Alexa Fluor 700	FAB80781N-100UG
Alexa Fluor 750	FAB80781S-100UG
Alexa Fluor Plus 405	FAB80781AFP405-100UG
Alexa Fluor Plus 488	FAB80781AFP488-100UG
Alexa Fluor Plus 555	FAB80781AFP555-100UG
Alexa Fluor Plus 594	FAB80781AFP594-100UG
Alexa Fluor Plus 647	FAB80781AFP647-100UG
Alexa Fluor Plus 680	FAB80781AFP680-100UG
Allophycocyanin	FAB80781A
Allophycocyanin/Cy7	FAB80781APCCY7
Biotin	FAB80781B
CoraFluor 1	FAB80781CL1
DyLight 350	FAB80781D
DyLight 405	FAB80781E
DyLight 488	FAB80781K
DyLight 550	FAB80781L
DyLight 594	FAB80781M
DyLight 650	FAB80781W
DyLight 680	FAB80781Y
DyLight 755	FAB80781Z
FITC	FAB80781F
HRP	FAB80781H
Janelia Fluor 525	FAB80781JF525
Janelia Fluor 549	FAB80781I
Janelia Fluor 646	FAB80781J
Janelia Fluor 669	FAB80781JF669
PE	FAB80781P
PE/Atto594	FAB80781PEATT594
PE/Cy5.5	FAB80781PECY55
PE/Cy7	FAB80781PECY7
PerCP	FAB80781C
mFluor Violet 450 SE	FAB80781MFV450
mFluor Violet 500 SE	FAB80781MFV500
mFluor Violet 610 SE	FAB80781MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB80781-SP
Carrier Free	FAB80781V-100UG
Carrier Free	FAB80781U-100UG
Carrier Free	FAB80781T-100UG
Carrier Free	FAB80781S-100UG
Carrier Free	FAB80781R-100UG
Carrier Free	FAB80781N-100UG
Carrier Free	FAB80781G-100UG

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NBP3-15237

Lgr5/GPR49 Antibody (4H8) - BSA Free

100 ul

NBP3-15237

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-54660

Lgr5/GPR49 Antibody - BSA Free

100 ul

NBP2-54660

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-54660-25ul

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NBP3-15445

Lgr5/GPR49 Antibody (9J9B8)

100 ul

NBP3-15445

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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page of 3

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Lgr5/GPR49 Antibodies

Lgr5/GPR49 Antibody - BSA Free

Lgr5/GPR49 Antibody (803420)

Lgr5/GPR49 Antibody (707042)

Lgr5/GPR49 Antibody - BSA Free

Lgr5/GPR49 Antibody - Azide and BSA Free

Lgr5/GPR49 Antibody (CL13567) - BSA Free

Lgr5/GPR49 Antibody (CL13579) - BSA Free

Lgr5/GPR49 Antibody (889901)

Lgr5/GPR49 Antibody (750835) [Unconjugated]

Lgr5/GPR49 Antibody (4H8) - BSA Free

Lgr5/GPR49 Antibody - BSA Free

Lgr5/GPR49 Antibody (9J9B8)