Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97491-0.2mg | |||
| BSA Free | NBP1-97491-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB500-181SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80607 | |||
| BSA Free | NB100-1830SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-537-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1974-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC8028G | |||
| Carrier Free | MAB8028-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC4240P | |||
| Carrier Free | IC4240A | |||
| Carrier Free | AF4240-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF902-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2736SS | |||
| Azide and BSA Free | NBP2-80845 | |||
![Western Blot: FGL2/Fibroleukin Antibody (6D9) [H00010875-M01] - FGL2 monoclonal antibody (M01), clone 6D9. Analysis of FGL2 expression in Raw 264.7.](http://images.novusbio.com/fullsize/FGL2-Fibroleukin-Antibody-6D9-Western-Blot-H00010875-M01-img0021.jpg "Western Blot: FGL2/Fibroleukin Antibody (6D9) [H00010875-M01] - FGL2 monoclonal antibody (M01), clone 6D9. Analysis of FGL2 expression in Raw 264.7.")
![Immunohistochemistry-Paraffin: FGL2/Fibroleukin Antibody (6D9) [H00010875-M01] - Analysis of monoclonal antibody to FGL2 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 0.5 ug/ml.](http://images.novusbio.com/fullsize/FGL2-Fibroleukin-Antibody-6D9-Immunohistochemistry-Paraffin-H00010875-M01-img0016.jpg)
![Western Blot: FGL2/Fibroleukin Antibody (6D9) [H00010875-M01] - FGL2 monoclonal antibody (M01), clone 6D9 Analysis of FGL2 expression in HeLa.](http://images.novusbio.com/fullsize/FGL2-Fibroleukin-Antibody-6D9-Western-Blot-H00010875-M01-img0019.jpg)
![Western Blot: FGL2/Fibroleukin Antibody (6D9) [H00010875-M01] - FGL2 monoclonal antibody (M01), clone 6D9. Analysis of FGL2 expression in PC-12.](http://images.novusbio.com/fullsize/FGL2-Fibroleukin-Antibody-6D9-Western-Blot-H00010875-M01-img0020.jpg)
![Western Blot: FGL2/Fibroleukin Antibody (6D9) [H00010875-M01] - Analysis of FGL2 expression in transfected 293T cell line by FGL2 monoclonal antibody (M01), clone 6D9.Lane 1: FGL2 transfected lysate(50 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/FGL2-Fibroleukin-Antibody-6D9-Western-Blot-H00010875-M01-img0022.jpg)
![Immunoprecipitation: FGL2/Fibroleukin Antibody (6D9) [H00010875-M01] - Analysis of FGL2 transfected lysate using anti-FGL2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FGL2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/FGL2-Fibroleukin-Antibody-6D9-Immunoprecipitation-H00010875-M01-img0017.jpg)
![ELISA: FGL2/Fibroleukin Antibody (6D9) [H00010875-M01] - Detection limit for recombinant GST tagged FGL2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FGL2-Fibroleukin-Antibody-6D9-ELISA-H00010875-M01-img0023.jpg)
![Western Blot: Spt6 Antibody [NB100-2582] - siRNA knockdown to confirm the specificity of Spt6 antibody (NB100-2582) in C2C12 myoblasts, WB: 1:2000 dilution. 20 ug whole cell lysate. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Spt6-Antibody-Western-Blot-NB100-2582-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Spt6 Antibody [NB100-2582] - siRNA knockdown to confirm the specificity of Spt6 antibody (NB100-2582) in C2C12 myoblasts, WB: 1:2000 dilution. 20 ug whole cell lysate. WB image submitted by a verified customer review.")
![<b>Genetic Strategies Validation. </b>Western Blot: Spt6 Antibody [NB100-2582] - SPT6 Depletion Induces lncRNA Transcription(A) YWHAZ locus showing pre-mRNA (– strand) & PROMPT (+ strand). SPT6 depletion induced lncRNA as shown by ChRNA-seq & mNET-seq analyses. The profile of mNuc-seq/H3K36me3 is shown below.(B) Meta-analysis of strand-specific ChrRNA-seq signals from −3 kb of TSS to +3 kb of transcription end site (TES) for divergent (pre-mRNA-PROMPT) genes.(C) Boxplots of PROMPT mNET-seq/total CTD & T4P signals.(D) Enhancer located 90 kb downstream of NR4A1 gene with neighboring gene ATG101 showing SPT6 depletion-induced eRNA by ChRNA-seq & mNET-seq. mNuc-seq/H3K4me1 & H3K4me3 signals indicate active enhancer & promoter, respectively.(E) Meta-analysis of eRNA from ChrRNA-seq & mNET-seq (Total) −3 kb to +3 kb from TSS (enhancers with highest eRNA levels selected).(F) Boxplots of eRNA mNET-seq/total CTD & T4P signals at TSS (–/+2 kb).(G & H) Western blot (G) & quantitative RT-PCR (H) of chromatin-bound RNA of parental & SETD2 CRISPR KO U2OS cells with indicated siRNA transfection for 48 hr. Data are represented as mean ± SEM.See also Figure S3. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30449723), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2582_rabbit-polyclonal-spt6-antibody-31020241552393.jpg)
![Western Blot: Spt6 Antibody [NB100-2582] - SPT6 Depletion Causes H3K36me3 Redistribution(A) Western blots with indicated antibodies 60 hr post-siSPT6 transfection (versus siLuc nonspecific control). EXOSC3 & H3 profiles shown as loading controls.(B) Meta-analysis of reads density (FPKM) for ratio of mNuc-seq/H3K36me3 with H3 at TSS of divergent PROMPT-pre-mRNA & enhancer RNA (eRNA) following SPT6 depletion. All subsequent transcription images employing siSPT6 versus siLuc are shown in red & blue, respectively.(C) Scatterplots of H3K36me3/H3 on pre-mRNA (0 to +3 kb), PROMPT (−3 kb to 0), & eRNA (−3 kb to +3 kb) regions in siLuc versus siSPT6. The percentage of upregulated regions by SPT6 depletion are indicated.(D) Boxplots of mNuc-seq/H3K36me3 ratio across pre-mRNA gene bodies, PROMPTs (3 kb from TSS), & eRNA (2 kb from center) & across lincRNA gene bodies.(E) Model of redistributed H3K36me3 marks caused by SPT6 depletion.See also Figure S2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30449723), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2582_rabbit-polyclonal-spt6-antibody-310202415532091.jpg)
![Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - analysis of Hsp47 in NIH/3T3 cell lysate (20ug/lane) using anti-Hsp47 antibody. 5x10^4 NIH/3T3 cells were seeded in 6-well plate and transfected with 50nM of HSP47 siRNA the following day. Total protein was harvested 72h after transfection. Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Western-Blot-NBP1-97491-img0006.jpg "Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - analysis of Hsp47 in NIH/3T3 cell lysate (20ug/lane) using anti-Hsp47 antibody. 5x10^4 NIH/3T3 cells were seeded in 6-well plate and transfected with 50nM of HSP47 siRNA the following day. Total protein was harvested 72h after transfection. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] - WNT and BMP signaling pathways play crucial roles in regeneration. a-d Real-time PCR for WNT and BMP signals, including Wnt7b Dual immunostaining for K15 and phospho-Smad1/5 (pSmad1/5) revealed the nuclear staining of pSmad1/5 in hair stem cells at day 7 when skin was stretched (arrow). Scale bar?=?50?um. h, i Immunostaining and confocal microscope. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09402-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Immunocytochemistry-Immunofluorescence-NBP1-97491-img0007.jpg)
![Immunohistochemistry-Frozen: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Hsp47 antibody in guinea pig skin (subdermis). Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Immunohistochemistry-Frozen-NBP1-97491-img0004.jpg)
![Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of HSP47, mAb (M16.10A1): Lane 1: Rat recombinant HSP47; Lane 2: Rat-2 (heat shocked); Lane 3: L929 (heat shocked); Lane 4: 3T3 (heat shocked); Lane 5: CHO (heat shocked); Lane 6: HeLa (heat shocked).](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Western-Blot-NBP1-97491-img0003.jpg)
![Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of paraffin-embedded tissue section of rat carotid artery 14 days after balloon-injury to the artery, stained using Hsp47 (Colligin) mAb (M16.10A1).](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Immunohistochemistry-Paraffin-NBP1-97491-img0001.jpg)
![Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of human breast cancer tissue immunohistochemically stained using Hsp47 mAb (M16.10A1).](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Immunohistochemistry-Paraffin-NBP1-97491-img0002.jpg)
![Simple Western: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Simple Western lane view shows a specific band for Hsp47 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Simple-Western-NBP1-97491-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] - WNT & BMP signaling pathways play crucial roles in regeneration. a–d Real-time PCR for WNT & BMP signals, including Wnt7b (a), Wnt10a (b), Lef1 (c), & Bmp2 (d) in response to stretch (day 1 & day 7) & release of stretch (day 9); n = 6 for each group. e Schematic of hair cycle activator & inhibitor in response to strain alteration. f Dual immunostaining for K15 & beta -catenin revealed nuclear staining of beta -catenin in hair follicles at day 9 & day 14 when stretch was released. Scale bar = 50 μm. g Dual immunostaining for K15 & phospho-Smad1/5 (pSmad1/5) revealed the nuclear staining of pSmad1/5 in hair stem cells at day 7 when skin was stretched (arrow). Scale bar = 50 μm. h, i Immunostaining (h) & confocal microscope (i) revealed co-localization of HSP47 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (h) or 10 μm (i). j, k Immunostaining (j) & confocal microscope (k) revealed co-localization of Perilipin-1 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (j) or 10 μm (k). Statistical significance was determined using ANOVA followed by a Bonferroni post hoc test. Data are presented as means ± SEM. *p < 0.05. **p < 0.01. ***p < 0.001. Source data are provided as a Source Data file. *Autofluorescence of hair shafts in g, h Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30944305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97491_mouse-monoclonal-hsp47-antibody-m16-10a1-310202416121964.jpg)
![Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] - WNT & BMP signaling pathways play crucial roles in regeneration. a–d Real-time PCR for WNT & BMP signals, including Wnt7b (a), Wnt10a (b), Lef1 (c), & Bmp2 (d) in response to stretch (day 1 & day 7) & release of stretch (day 9); n = 6 for each group. e Schematic of hair cycle activator & inhibitor in response to strain alteration. f Dual immunostaining for K15 & beta -catenin revealed nuclear staining of beta -catenin in hair follicles at day 9 & day 14 when stretch was released. Scale bar = 50 μm. g Dual immunostaining for K15 & phospho-Smad1/5 (pSmad1/5) revealed the nuclear staining of pSmad1/5 in hair stem cells at day 7 when skin was stretched (arrow). Scale bar = 50 μm. h, i Immunostaining (h) & confocal microscope (i) revealed co-localization of HSP47 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (h) or 10 μm (i). j, k Immunostaining (j) & confocal microscope (k) revealed co-localization of Perilipin-1 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (j) or 10 μm (k). Statistical significance was determined using ANOVA followed by a Bonferroni post hoc test. Data are presented as means ± SEM. *p < 0.05. **p < 0.01. ***p < 0.001. Source data are provided as a Source Data file. *Autofluorescence of hair shafts in g, h Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30944305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97491_mouse-monoclonal-hsp47-antibody-m16-10a1-31020241613615.jpg)
![Western Blot: Eg5 Antibody [NB500-181] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Eg5.](http://images.novusbio.com/fullsize/Eg5-Antibody-Western-Blot-NB500-181-img0005.jpg "Western Blot: Eg5 Antibody [NB500-181] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Eg5.")
![Immunocytochemistry/Immunofluorescence: Eg5 Antibody [NB500-181] - Staining of HeLa cells fixed in 3.5% paraformaldehyde. Cytoplasmic staining during interphase and spindle staining during mitosis.](http://images.novusbio.com/fullsize/Eg5-Antibody-Immunocytochemistry-Immunofluorescence-NB500-181-img0003.jpg)
![Western Blot: Eg5 Antibody [NB500-181] - Human Eg5 rescues depletion of the Drosophila kinesin-5 family member Klp61F. Western blot analysis of cell lysates prepared from induced and uninduced; control and Klp61F RNAi-treated cells blotted with anti-Eg5 to monitor induction of Eg5-mCherry, anti-Klp61F to confirm depletion of the motor and anti-alpha-tubulin as a loading control. Cells depleted of Klp61F and with Eg5-mCherry induced are referred to as RepEg5 cells. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fonc.2013.00187/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Eg5-Antibody-Western-Blot-NB500-181-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Eg5 Antibody [NB500-181] - Spontaneous assembly of rosettes in iChM5 derivatives.Feeder free cultures iChM5Ap15 & iChM5Bp28 cells in chamber slides were immunostained as indicated. Left panel, emerging rosettes among self-renewing iPSCs; grayscale inset shows 1x magnification of immunostaining of Oct4 alone. Middle panel shows Nanog staining alone with inset at 2X magnification showing presumptive centrosomes (arrows). Bottom left panel shows forming rosettes immunopositive for Sox2 & Eg5. Bottom right panel shows low magnification image of immunostaining of Sox2 in this iChM5Ap15 culture, showing that virtually all cells were immunopositive. Grey scale inset shows representative forming rosette. Asterisks (*) in each panel indicates example of forming rosette. Scale bar, 50 microns. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25426336), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-181_rabbit-polyclonal-eg5-antibody-310202416171491.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0012.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0011.jpg)
![Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Simple-Western-NB100-1830-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241003248.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-1772024959417.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241044639.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on HEK293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-177202495941.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720249362617.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0008.jpg "Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Western-Blot-NB300-537-img0006.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Hek293 cells with PPAR alpha/NR1C1 [3B6/PPAR] Antibody NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0009.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - 293T cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Western-Blot-NB300-537-img0002.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Jurkat cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0003.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Hela cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0004.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in 3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0005.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Jurkat cells with PPAR alpha/NR1C1 Antibody (3B6/PPAR) NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free [NB300-537] - PPAR alpha/NR1C1 was knocked down with siRNA in primary cultured rat cardiomyocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-BSA-Free-Knockdown-Validated-NB300-537-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235] - IF analysis of BrdU in Proteinase K pretreated mouse lacrimal gland. Image courtesy of anonymous customer review.](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-Immunocytochemistry-Immunofluorescence-NB500-235-img0006.jpg "Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235] - IF analysis of BrdU in Proteinase K pretreated mouse lacrimal gland. Image courtesy of anonymous customer review.")
![Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235] - Lysate was immunoprecipitated with anti-brdu antibody (NB500-235), 5-methyl cytosine (5-Me), or a no antibody control (No A). An ELISA was then performed to measure total activity.](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-Immunoprecipitation-NB500-235-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1974] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1974) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1974-img0012.jpg "Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1974] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1974) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1974] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1974) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1974-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1974] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1974) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1974-img0014.jpg)
![Western Blot: Calnexin Antibody [NB100-1974] - Identification of plasma exosomes and differentially expressed exosomal miRNAs. Confirmation of the exosomes markers with Western blotting indicated the presence of CD63 but the absence of calnexin in exosomes. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fonc.2019.00459/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NB100-1974-img0010.jpg)
![Immunohistochemistry: Calnexin Antibody [NB100-1974] - Analysis of Calnexin in mouse bladder using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-NB100-1974-img0005.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1974] - An intracellular stain was performed on rat FR cells with Calnexin Antibody NB100-1974 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1974-img0017.jpg)
![Western Blot: Calnexin Antibody [NB100-1974] - Analysis of Calnexin in HeLa whole cell lysate.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NB100-1974-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1974] - Zebrafish Vwf forms pseudo-Weibel-Palade bodies (pseudo-WPBs) in mammalian cell culture. pzVwf/Myc-HIS (zebrafish Vwf) plasmids were transfected into HEK293T cells. Anti-Myc antibody conjugated to Alexa Fluor 488 (green channel) was used for detection and anti-calnexin antibody conjugated to Alexa Fluor 594 (red channel) labeled endoplasmic reticulum (ER). Both constructs demonstrate formation of elongated Myc positive and ER negative structures (absence of yellow signal in the merged panels) characteristic of pseudo-WPBs. Scale bars, 2.5 um. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ah/2012/214209/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1974-img0011.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1974] - Analysis of Alexa Fluor (R) 488 conjugate of NB100-1974. An intracellular stain was performed on Jurkat cells with Calnexin antibody NB100-1974AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1974-img0008.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1974] - An intracellular stain was performed on HeLa cells with Calnexin antibody NB100-1974AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1974-img0009.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1974] - An intracellular stain was performed on Daudi cells with Calnexin Antibody NB100-1974 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1974-img0015.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1974] - An intracellular stain was performed on NIH3T3 cells with Calnexin Antibody NB100-1974 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1974-img0016.jpg)
![Simple Western: Calnexin Antibody [NB100-1974] - Image shows a specific band for Calnexin in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Simple-Western-NB100-1974-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Calnexin Antibody - BSA Free [NB100-1974] - Zebrafish Vwf forms pseudo-Weibel-Palade bodies (pseudo-WPBs) in mammalian cell culture. pVWF/Myc-HIS (human VWF, (a–c)) or pzVwf/Myc-HIS (zebrafish Vwf, (d–i)) plasmids were transfected into HEK293T cells. Anti-Myc antibody conjugated to Alexa Fluor 488 (green channel, (a, d, g)) was used for detection & anti-calnexin antibody conjugated to Alexa Fluor 594 (red channel, (b, e, h)) labeled endoplasmic reticulum (ER). Both constructs demonstrate formation of elongated Myc positive & ER negative structures (absence of yellow signal in the merged panels, (c, f, i)) characteristic of pseudo-WPBs (examples are indicated in (a, d), & (g) by arrowheads). Scale bars, 2.5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23049555), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1974_rabbit-polyclonal-calnexin-antibody-310202415293329.jpg)
![Immunocytochemistry/ Immunofluorescence: Calnexin Antibody - BSA Free [NB100-1974] - Zebrafish Vwf forms pseudo-Weibel-Palade bodies (pseudo-WPBs) in mammalian cell culture. pVWF/Myc-HIS (human VWF, (a–c)) or pzVwf/Myc-HIS (zebrafish Vwf, (d–i)) plasmids were transfected into HEK293T cells. Anti-Myc antibody conjugated to Alexa Fluor 488 (green channel, (a, d, g)) was used for detection & anti-calnexin antibody conjugated to Alexa Fluor 594 (red channel, (b, e, h)) labeled endoplasmic reticulum (ER). Both constructs demonstrate formation of elongated Myc positive & ER negative structures (absence of yellow signal in the merged panels, (c, f, i)) characteristic of pseudo-WPBs (examples are indicated in (a, d), & (g) by arrowheads). Scale bars, 2.5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23049555), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1974_rabbit-polyclonal-calnexin-antibody-310202415392534.jpg)
![Immunocytochemistry/ Immunofluorescence: Calnexin Antibody - BSA Free [NB100-1974] - Zebrafish Vwf forms pseudo-Weibel-Palade bodies (pseudo-WPBs) in mammalian cell culture. pVWF/Myc-HIS (human VWF, (a–c)) or pzVwf/Myc-HIS (zebrafish Vwf, (d–i)) plasmids were transfected into HEK293T cells. Anti-Myc antibody conjugated to Alexa Fluor 488 (green channel, (a, d, g)) was used for detection & anti-calnexin antibody conjugated to Alexa Fluor 594 (red channel, (b, e, h)) labeled endoplasmic reticulum (ER). Both constructs demonstrate formation of elongated Myc positive & ER negative structures (absence of yellow signal in the merged panels, (c, f, i)) characteristic of pseudo-WPBs (examples are indicated in (a, d), & (g) by arrowheads). Scale bars, 2.5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23049555), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1974_rabbit-polyclonal-calnexin-antibody-310202415384162.jpg)
![Immunocytochemistry/ Immunofluorescence: Calnexin Antibody - BSA Free [NB100-1974] - Zebrafish Vwf forms pseudo-Weibel-Palade bodies (pseudo-WPBs) in mammalian cell culture. pVWF/Myc-HIS (human VWF, (a–c)) or pzVwf/Myc-HIS (zebrafish Vwf, (d–i)) plasmids were transfected into HEK293T cells. Anti-Myc antibody conjugated to Alexa Fluor 488 (green channel, (a, d, g)) was used for detection & anti-calnexin antibody conjugated to Alexa Fluor 594 (red channel, (b, e, h)) labeled endoplasmic reticulum (ER). Both constructs demonstrate formation of elongated Myc positive & ER negative structures (absence of yellow signal in the merged panels, (c, f, i)) characteristic of pseudo-WPBs (examples are indicated in (a, d), & (g) by arrowheads). Scale bars, 2.5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23049555), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1974_rabbit-polyclonal-calnexin-antibody-310202415291966.jpg)
![Western Blot: Calnexin Antibody - BSA Free [NB100-1974] - Liver-specific disruption of Chrebp in mice. A: Quantitative real-time PCR analysis of ChREBP mRNA. Total RNA was isolated from various tissues of control & liver-specific Chrebp knockout (L-Chrebp−/−) mice & subjected to real-time PCR analysis with 36B4 as the invariant control. Each value represents the mean ± SEM of four mice relative to that of controls, which was arbitrarily defined as 1.0. #P < 0.01 denotes the level of statistical significance (two-tailed Student’s t-test) between control & L-Chrebp−/− mice. B: Immunoblot analysis of ChREBP in liver lysates of control & L-Chrebp−/− mice. Aliquots (60 μg of protein) of liver whole-cell lysates were subjected to SDS-PAGE & immunoblot analysis with anti-ChREBP & anti-calnexin antibodies. ChREBP delta denotes a truncated aberrant ChREBP protein present only in lysates prepared from L-Chrebp−/− livers. The functional domains of ChREBP, including the glucose-sensing proline-rich bHLH-Zip DNA-binding & ZIP-like domains, are denoted. NLS, nuclear localization sequence. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0022227520331369), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1974_rabbit-polyclonal-calnexin-antibody-310202415535130.jpg)
![Western Blot: Calnexin Antibody - BSA Free [NB100-1974] - Immunoblot analysis of livers from control & L-Chrebp−/− mice subjected to fasting & refeeding with a high-sucrose diet. Littermate control & L-Chrebp−/− mice (same as those described in supplemental Tables S2A & S2B) were subjected to fasting & refeeding. The nonfasted (N) groups were fed chow diet ad libitum. The fasted (F) group was fasted 12 h, & the refed (R) group was fasted for 12 h & then refed with 60% (w/w) high-sucrose diet for 12 h prior to study. Liver whole-cell lysates & membrane fractions were prepared individually, & equal amounts of protein from each mouse of the same group (four per group) were pooled. Aliquots (40 μg for whole-cell lysates & 30 μg for membrane fractions) of the pooled protein were subjected to SDS-PAGE & immunoblot analysis. Immunoblot analysis of Insig-1 & Insig-2 were carried out using membrane fractions. Whole-cell lysates were used to detect other proteins. The precursor & nuclear forms of SREBPs are denoted as P & N, respectively. Calnexin was used as loading control. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0022227520331369), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1974_rabbit-polyclonal-calnexin-antibody-31020241553732.jpg)
![Simple Western: GFP Antibody [Unconjugated] [AF4240] - Design for a membrane-localized ACE2 expression system. (A) Our ACE2 construct is driven by a CMV promoter followed by the first 25 residues of ACE2 containing the leader sequence that direct ACE2 to the plasma membrane. This is followed by a 3xHA tag linked to the remainder of ACE2 (20-805) and a C-terminal sfGFP. Both 3xHA and sfGFP fusions are separated from ACE2 by flexible 3xGGGGS linkers. (B) The ACE2 fusion protein is designed to be embedded in the plasma membrane where it can perform extracellular carboxypeptidase-mediated metabolism and its levels can be detected by cell staining with antibodies to HA. (C) Lysates from untransfected or 3xHA-ACE2-sfGFP-transfected HEK293 cells were analyzed by automated Jess capillary immunoassay using antibodies to HA, GFP, and two ACE2 antibodies. (D) Confocal fluorescence microscopy of HEK cells transfected with 3xHA-ACE2-sfGFP and stained with HA and the nuclear stain DAPI. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37644110), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af4240_gfp-affinity-purified-polyclonal-ab-30820241044553.jpg)
![Western Blot: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Daudi whole cell protein was separated by SDS-PAGE on a 7.5% gel and transferred to PVDF membrane. The membrane was probed with anti-MDM2 antibody at 2 ug/ml and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-SMP14-Western-Blot-NB100-2736-img0001.jpg "Western Blot: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Daudi whole cell protein was separated by SDS-PAGE on a 7.5% gel and transferred to PVDF membrane. The membrane was probed with anti-MDM2 antibody at 2 ug/ml and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-SMP14-Immunohistochemistry-Paraffin-NB100-2736-img0003.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-SMP14-Immunohistochemistry-Paraffin-NB100-2736-img0002.jpg)