Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24733SS | |||
| Azide and BSA Free | NBP2-33258 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0700A488-100 | |||
| Alexa Fluor 546 | DDX0700A546-100 | |||
| Alexa Fluor 647 | DDX0700A647-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56674SS | |||
| Azide and BSA Free | NBP2-33241 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1936SS | |||
| Azide and BSA Free | NBP2-80955 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47491SS | |||
| Azide and BSA Free | NBP2-81005 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24588SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77668SS | |||
| Azide and BSA Free | NBP2-80903 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1327SS | |||
| Azide and BSA Free | NBP2-80626 | |||
![Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Amino acid E28 on Bif-1 is important for calpain-mediated processing and autophagic clearance of p62. The myc-tagged constructs: wild-type Endophilin B1/Bif-1 and E28A Endophilin B1/Bif-1 were transfected into H1299 cells. 24h later, the cells were treated or not with 100nM thapsigargin for 1h. Afterwards, the cell lysates were prepared and analyzed by western blot with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//bio.biologists.org/content/6/5/551.long), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-30A882-1-1-Western-Blot-NBP2-24733-img0009.jpg "Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Amino acid E28 on Bif-1 is important for calpain-mediated processing and autophagic clearance of p62. The myc-tagged constructs: wild-type Endophilin B1/Bif-1 and E28A Endophilin B1/Bif-1 were transfected into H1299 cells. 24h later, the cells were treated or not with 100nM thapsigargin for 1h. Afterwards, the cell lysates were prepared and analyzed by western blot with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//bio.biologists.org/content/6/5/551.long), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using 5 ug/ml conc. of Endophilin B1 antibody (clone 30A882.1.1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Endophilin B1 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-30A882-1-1-Immunohistochemistry-Paraffin-NBP2-24733-img0008.jpg)
![Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Analysis for Bi-f-1/Endophilin-B1 using this antibody at 2 ug/ml dilution against 10 ug of Jurkat cell lysate.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-30A882-1-1-Western-Blot-NBP2-24733-img0006.jpg)
![Simple Western: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Simple Western lane view shows a specific band for Endophilin B1/Bif-1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-30A882-1-1-Simple-Western-NBP2-24733-img0004.jpg)
![Western Blot: V5 Epitope Tag Antibody [NB600-380] - Detection of V5-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-V5 antibody NB600-380 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Western-Blot-NB600-380-img0005.jpg "Western Blot: V5 Epitope Tag Antibody [NB600-380] - Detection of V5-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-V5 antibody NB600-380 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Flow-Cytometry-DDX0700P-100-img0003.jpg "Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34")
![Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of R34-34-transfected Cos7 cells](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Flow-Cytometry-DDX0700P-100-img0002.jpg)
![Immunoprecipitation: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - R34-34 antigen from 125I-surface labelled Pre-Alp cells](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Immunoprecipitation-DDX0700P-100-img0001.jpg)
![Immunohistochemistry-Paraffin: p73 Antibody (5B1288) [NB100-56674] - Human breast cancer section using 10ug/mL of p73 antibody (clone 5B1288) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). The cancer cells showed cytoplasmic immunoreactivity for p73 and the signal was very weak in the core and stroma of tumors. Peripheral cells of tumor areas, apparently the myoepithelial cells, showed a strong nuclear staining of p73.](http://images.novusbio.com/fullsize/p73-Antibody-5B1288-Immunohistochemistry-Paraffin-NB100-56674-img0008.jpg "Immunohistochemistry-Paraffin: p73 Antibody (5B1288) [NB100-56674] - Human breast cancer section using 10ug/mL of p73 antibody (clone 5B1288) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). The cancer cells showed cytoplasmic immunoreactivity for p73 and the signal was very weak in the core and stroma of tumors. Peripheral cells of tumor areas, apparently the myoepithelial cells, showed a strong nuclear staining of p73.")
![Western Blot: p73 Antibody (5B1288) [NB100-56674] - Analysis of the expression level of p73 isoforms. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/11774) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p73-Antibody-5B1288-Western-Blot-NB100-56674-img0010.jpg)
![Immunohistochemistry-Paraffin: p73 Antibody (5B1288) [NB100-56674] - Human breast cancer section using 10 ug/mL of p73 antibody (clone 5B1288) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). The cancer cells showed cytoplasmic immunoreactivity for p73 and the signal was very weak in the core and stroma of tumors. Peripheral cells of tumor areas, apparently the myoepithelial cells, showed a strong nuclear staining of p73.](http://images.novusbio.com/fullsize/p73-Antibody-5B1288-Immunohistochemistry-Paraffin-NB100-56674-img0009.jpg)
![Western Blot: p73 Antibody (5B1288) [NB100-56674] - Analysis of p73 in transfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/p73-Antibody-5B1288-Western-Blot-NB100-56674-img0004.jpg)
![Western Blot: p73 Antibody (5B1288) [NB100-56674] - Analysis of p73 in HeLa cell lysate (Cat no. NBP2-25045) using this antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/p73-Antibody-5B1288-Western-Blot-NB100-56674-img0006.jpg)
![Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. SMN proteins were found throughout the cytoplasm and as nuclear foci in control sensory neurons. SMN nuclear foci localized within CBs (coilin) in controls (upper panels, white arrows), whereas in the diabetic nucleus the numerous CBs were present but lost their colocalization with SMN nuclear foci (lower panels, yellow arrows). Arrowheads indicate sensory neurons magnified in the insets. Scale bars: 20 um, 10 um in insets. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/lookup/doi/10.1242/dmm.028225), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunohistochemistry-NB100-1936-img0007.jpg "Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. SMN proteins were found throughout the cytoplasm and as nuclear foci in control sensory neurons. SMN nuclear foci localized within CBs (coilin) in controls (upper panels, white arrows), whereas in the diabetic nucleus the numerous CBs were present but lost their colocalization with SMN nuclear foci (lower panels, yellow arrows). Arrowheads indicate sensory neurons magnified in the insets. Scale bars: 20 um, 10 um in insets. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/lookup/doi/10.1242/dmm.028225), licensed under a CC-BY license.")
![Western Blot: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN expression in 1) HeLa, 2) NTera2, 3) HepG2, 4) MCF7, 5) NIH 3T3, 6) PC12, and 7) COS7 whole cell lysates.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Western-Blot-NB100-1936-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB100-1936 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - scIF of control (f) and etv5b MO (g) cells from pmnx1:eGFP embryos. H2B, GFP and Smn signals are shown in Z-projected confocal sections. GFP+ cells marked by white rectangles are magnified on the right. Scale bars: 10 um for low and 5 um for high magnification. Image collected and cropped by Citeab from the following publication (Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Sci Rep (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with SMN Antibody [2B1] conjugated to Alexa Fluor 488 (NB100-1936AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0012.jpg)
![Flow Cytometry: SMN Antibody (2B1) [NB100-1936] - An intracellular stain was performed on Neuro2a cells with SMN Antibody (2B1) NB100-1936 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Flow-Cytometry-NB100-1936-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - The SMN antibody was tested at a 1:250 dilution in HeLa cells against DyLight 488 (Green). Actin nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0004.jpg)
![Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN on mouse brain using NB100-1936.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunohistochemistry-NB100-1936-img0001.jpg)
![Flow Cytometry: SMN Antibody (2B1) [NB100-1936] - An intracellular stain was performed on Ntera2 cells with SMN Antibody (2B1) NB100-1936 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Flow-Cytometry-NB100-1936-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - CWC22 is colocalized with nuclear speckles & upregulated in diabetic DRG sensory neurons. (A) Subcellular distribution of TDP-43 & CWC22 in control DRG sensory neurons. TDP-43 was stained diffusely in the nucleus, excluding SMN foci in sensory neurons. CWC22 consistently colocalized with a marker protein SC35 of nuclear speckles in sensory neurons. No obvious differences in the subcellular localization of CWC22 were identified in diabetic neurons (not shown) compared with controls. Scale bar: 10 μm. (B) qRT-PCR analysis of Cwc22 mRNA expression in diabetic & control mice. Cwc22 expression was upregulated ∼2.5-fold in diabetic DRGs. *P<0.05, unpaired two-tailed Student's t-test. Data represented as mean±s.e.m. See Cheng et al. (2015) for microarray data indicating rises in Cwc22 expression as reported separately. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/dmm/article/10/3/215/2257/Diabetic-polyneuropathy-sensory-neurons-nuclear), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1936_mouse-monoclonal-smn-antibody-2b1-3102024168396.jpg)
![Western Blot: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Using VCAM1 mouse mAb against (1) HUVEC and (2) EC cell lysate.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-6G9-Western-Blot-NBP1-47491-img0006.jpg "Western Blot: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Using VCAM1 mouse mAb against (1) HUVEC and (2) EC cell lysate.")
![Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Photomicrographs of two separate gut sections from a patient with EHEC colitis. Panels (C) and (D) are stained to show VCAM-1/CD106 expression in endothelium, indicating inflammatory activation (40x magnification). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0055278), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-6G9-Immunohistochemistry-NBP1-47491-img0010.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - (A) human lung cancer and (B) colon cancer using VCAM1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-6G9-Immunohistochemistry-Paraffin-NBP1-47491-img0004.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Human placenta tissues using VCAM1 mouse mAb.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-6G9-Immunohistochemistry-Paraffin-NBP1-47491-img0005.jpg)
![Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Breast carcinoma, cytoplasmic staining. IHC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-6G9-Immunohistochemistry-NBP1-47491-img0007.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - FFPE section of human lung cancer. Image at 20X magnification. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-6G9-Immunohistochemistry-Paraffin-NBP1-47491-img0009.jpg)
![Simple Western: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Lane view shows a specific band for CD106/VCAM1 in 0.5 mg/mL of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-6G9-Simple-Western-NBP1-47491-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491] - Enhanced expression of endothelial SENP1 & GATA2 correlates w/ graft arteriosclerosis (GA) progression.Similarly sized human coronary arteries w/ GA from chronically rejecting heart allografts or w/out disease from non-transplanted hearts collected & evaluated by histological analysis. (a,b) Dramatically increased expression of endothelial SENP1 detected in the diseased vessel wall. Endothelial SENP1 expression is demonstrated by immunofluorescence analysis of coronary artery cross-sections that stained for SENP1 & the endothelial marker PECAM-1 w/ DAPI labelling of the nuclei. Representative images are shown in (a) w/ quantification data in (b). Bar represents 50 μm. (c) Induction of endothelial adhesion molecules resulted in a similar augmented pattern as endothelial SENP1. Representative images of immunofluorescence staining for VCAM-1 or P-selectin & PECAM-1 in coronary arteries w/ DAPI counterstaining are shown. Bar represents 50 μm. (d,e) Expression of endothelial GATA2 elevated w/ the progression of aggravated rejection. Representative images of the immunofluorescence staining of coronary arteries for GATA2 & PECAM-1 together w/ DAPI nuclear staining are shown in (d) w/ quantification data in (e). Negative SENP1 & GATA2 staining in the isotype or blocking peptide controls are also shown in (a) & (d). Bar represents 50 μm. Data presented in (b,e) are the mean±s.e.m. from five separate clinical specimens per group as indicated. *P<0.05, **P<0.01 & ***P<0.0001; one-way ANOVA followed by Bonferroni test. GA, graft arteriosclerosis; PECAM, platelet/endothelial cell adhesion molecule; SENP, sentrin-specific protease; VCAM, vascular cell adhesion molecule. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15426), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47491_mouse-monoclonal-vcam-1-cd106-antibody-6g9-310202416161834.jpg)
![Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491] - Loss of endothelial SENP1 inhibits EC activation.(a) Grafts from WT or SENP1-ecKO mice were harvested 3 days post-transplantation. The induction of endothelial adhesion molecules was demonstrated by immunofluorescence staining of ICAM-1, VCAM-1, or P-selectin & PECAM-1 with DAPI labelling of the nuclei. Bar represents 50 μm. (b–e) Attenuated induction of adhesion molecules in SENP1-ecKO MAECs. Flow cytometry analysis of ICAM-1, VCAM-1 & P-selectin in MAECs isolated from WT or SENP1-ecKO mice after TNF or IL-1 beta treatment. Representative histograms are shown in (b) with the quantification of mean intensity in (c–e). (f–h) Overexpression of the catalytically inactive form of SENP1 (SENP1-Mut) inhibits the induction of adhesion molecules in HUVECs. HUVECs were infected by Ad-SENP1-Mut or vector control (Ad-LacZ) for 24 h, treated with pro-inflammatory cytokines & analysed by flow cytometry in the same way as MAECs. Representative histograms of ICAM-1 & VCAM-1 are shown in (f) with the quantification of mean intensity in (g,h). Data are presented as the mean±s.e.m. from at least three independent experiments. *P<0.05 & **P<0.01; two-way ANOVA followed by Bonferroni post-test. MAEC, mouse aortic endothelial cell. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15426), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47491_mouse-monoclonal-vcam-1-cd106-antibody-6g9-31020241616185.jpg)
![Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 Antibody [NBP2-24588]. Human pancreas probed with RIPK3/RIP3 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Immunohistochemistry-Paraffin-NBP2-24588-img0004.jpg "Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 Antibody [NBP2-24588]. Human pancreas probed with RIPK3/RIP3 antibody at 10 ug/ml.")
![<b>Biological Strategies Validation. </b>Western Blot: RIPK3/RIP3 Antibody [NBP2-24588] - Representative western blot analysis of RIP1, RIPK3/RIP3, and beta-actin in WI-38 NCs and IR-SCs 24 h after incubation with Veh or 10 uM PL. A cell lysate of etoposide-treated Jurkat cells was used as a positive control. Image collected and cropped by CiteAb from the following publication (//www.aging-us.com/article/101100/text) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP2-24588-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RIPK3/RIP3 Antibody [NBP2-24588] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with RIPK3/RIP3 Antibody [NBP2-24588] at a 1:200 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used at a 1:1000 dilution as a co-stain and detected with DyLight 550 (Red) at a 1:500 dilution. Nuclei were detected with DAPI (Blue) at 2.0ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24588-img0003.jpg)
![Western Blot: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 antibody [NBP2-24588]. Human tissue blot probed with 5 ug/ml of RIP3 antibody: 1) brain, 2) heart, 3) small intestine, 4) kidney, 5) liver, 6) lung, 7) skeletal muscle, 8) stomach, 9) spleen, 10) ovary, 11) testis, 12) placenta probed at 5 ug/ml, 13) Ramos cell lysate probed at 7 ug/ml. Theoretical molecular weight 56.7 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP2-24588-img0005.jpg)
![Western Blot: RIPK3/RIP3 Antibody - BSA Free [NBP2-24588] - HOIP limits the activity of TRAIL‐induced apoptosis‐ & necroptosis‐mediating signalling complexesControl & HOIP KO HeLa cells were treated with FLAG‐lz‐TRAIL (500 ng/ml) for the indicated times. The TRAIL complex I was immunoprecipitated via anti‐FLAG beads. Complex II was isolated by immunoprecipitating caspase‐8 from complex I‐depleted lysates. Western blot was performed using the indicated antibodies. * indicates unspecific bands.WT & HOIP‐deficient TNF KO MEFs, pre‐treated for 1 h with zVAD, were stimulated with iz‐TRAIL (1 μg/ml) for the indicated times. FADD‐containing complexes were immunoprecipitated & analysed by Western blot.Data information: See also Fig EV2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28258062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-24588_rabbit-polyclonal-ripk3-rip3-antibody-31020241534394.jpg)
![Western Blot: RIPK3/RIP3 Antibody - BSA Free [NBP2-24588] - The catalytic activity of HOIP contributes to preventing TRAIL‐induced apoptosis but is dispensable for preventing necroptosisLysates from control or HOIP KO K562 cells reconstituted w/ empty vector, HOIP WT or HOIP C885S analysed by WB.HOIP KO K562 cells reconstituted w/ empty vector, HOIP WT or HOIP C885S, pre‐treated w/ zVAD and/or Nec‐1s for 1 h as indicated, treated w/ iz‐TRAIL (1 μg/ml) for 24 h. Percentage of cell death determined by flow cytometry after PI labelling (n = 5; mean ± SEM). *P < 0.05, **P < 0.01; statistics performed using ANOVA.Control & HOIP KO K562 cells reconstituted w/ empty vector, HOIP WT or HOIP C885S treated w/ iz‐TRAIL (100 ng/ml) for the indicated times. Lysates analysed w/ the indicated antibodies.Lysates from TNF KO HOIP KO MEFs reconstituted w/ empty vector, HOIP WT or HOIP C885S analysed by WB.TNF KO HOIP KO MEFs reconstituted w/ empty vector, HOIP WT or HOIP C885S, pre‐treated w/ zVAD & Nec‐1s for 1 h as indicated, treated w/ iz‐TRAIL (1 μg/ml) for 24 h. Percentage of cell death determined by flow cytometry after PI labelling (n = 4; mean ± SEM). *P < 0.05, ***P < 0.001; statistics performed using ANOVA.TNF KO HOIP KO MEFs reconstituted w/ empty vector, pre‐treated w/ zVAD and/or the RIPK3 inhibitor GSK'872 for 1 h as indicated, treated w/ iz‐TRAIL (1 μg/ml) for 24 h. Percentage of cell death determined by flow cytometry after PI labelling (n = 3; mean ± SEM). *P < 0.05; statistics performed using t‐test.TNF KO HOIP KO MEFs reconstituted w/ empty vector, HOIP WT or HOIP C885S, pre‐treated w/ zVAD for 1 h, treated w/ iz‐TRAIL (1 μg/ml) for the indicated times. FADD‐containing complexes immunoprecipitated & analysed by WB. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28258062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-24588_rabbit-polyclonal-ripk3-rip3-antibody-310202416114733.jpg)
![Western Blot: Glut1 Antibody [NB300-666] - Western blot analysis of 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and HIF1B (NB100-124) analysis, nuclear extracts were generated and analyzed. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY license. Glut-1 was detected using NB300-666 in whole cell extracts.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB300-666-img0007.jpg "Western Blot: Glut1 Antibody [NB300-666] - Western blot analysis of 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and HIF1B (NB100-124) analysis, nuclear extracts were generated and analyzed. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY license. Glut-1 was detected using NB300-666 in whole cell extracts.")
![Western Blot: Glut1 Antibody [NB300-666] - WB analysis of purified Glucose Transporter (GLUT1) with GLUT1 antibody at a dilution of 1:2000.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB300-666-img0001.jpg)
![Western Blot: Glut1 Antibody [NB300-666] - Upregulation of Glut1 (NB300-666) and Glut3 (NBP2-66872) expression by Aqp9 gene deletion and ONC. Western blots for Glut1 and Glut3 protein expression levels in WT and Aqp9 KO mice with and without ONC. beta actin was used as a control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32748371/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB300-666-img0008.jpg)
![Western Blot: Glut1 Antibody [NB300-666] - Rat brain cortical extracts (10 ug total protein per lane) on 10% SDS-PAGE gel. Antibody diluted 1:2000.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB300-666-img0006.jpg)
![Western Blot: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Total protein from human Colon, Thyroid and Stomach was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Panendothelial Cell Antigen (NB100-77668) in blocking buffer and detected with an anti-rat HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Panendothelial-Cell-Antigen-Antibody-MECA-32-Western-Blot-NB100-77668-img0009.jpg "Western Blot: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Total protein from human Colon, Thyroid and Stomach was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Panendothelial Cell Antigen (NB100-77668) in blocking buffer and detected with an anti-rat HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunohistochemistry-Paraffin: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Panendothelial Cell Antigen (MECA-32) was detected in immersion fixed paraffin-embedded sections of human spleen. Antibody at 1:500 for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte(TM) HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the endothelial cells.](http://images.novusbio.com/fullsize/Panendothelial-Cell-Antigen-Antibody-MECA-32-Immunohistochemistry-Paraffin-NB100-77668-img0008.jpg)
![Flow Cytometry: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Analysis using the PerCP conjugate of NB100-77668. Staining of bEND.3 cells with MECA-32 AF488.](http://images.novusbio.com/fullsize/Panendothelial-Cell-Antigen-Antibody-MECA-32-Flow-Cytometry-NB100-77668-img0007.jpg)
![Western Blot: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Mouse embryonic cell lysate (A). Antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/Panendothelial-Cell-Antigen-Antibody-MECA-32-Western-Blot-NB100-77668-img0006.jpg)
![Western Blot: LC3 Antibody Pack [NB910-40435] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40435-img0013.jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3 Antibody Pack [NB910-40435] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Rapamycin increases autophagy in brains of PDAPP mice. Representative epifluorescent (c200x) image of hippocampal CA1 in control- and rapamycin-fed transgenic PDAPP mice stained with an anti-LC3 antibody. An increase in LC3-immunoreactive puncta was observed in CA1 projections of transgenic PDAPP mice following rapamycin administration. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0009979) licensed under a CC-BY license. LC3A Antibody [NB100-2331]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: LC3 Antibody Pack [NB910-40435] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40435-img0014.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NB910-40435] - High autophagosome concentration is consumed during early immortalized human mesenchymal stem cell differentiation. Immortalized human mesenchymal stem cells were differentiated under osteogenic conditions (see Materials and methods) and assayed for changes in LC3I and LC3II during a 72-hour window. Cells were differentiated under standard conditions (top) or with addition of 5 uM rapamycin (middle) or 5 nM bafilomycin (bottom) for the first 3 hours of differentiation to modulate autophagy. Immunoblots were performed for LC3 at the indicated time points to assess autophagosome degradation via relative changes in LC3II (lower band; 17 kDa). Studies were repeated three times with similar trends seen consistently. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/5/6/140), licensed under a CC-BY license. LC3A Antibody [NB100-2331]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Western-Blot-NB910-40435-img0011.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Localization of LC3 by immunohistochemical method. The immunohistochemical staining shows that LC3, CRF, and HIF-1alpha have an overlapping localization in villous and extravillous trophoblast. d: decidua; v: villi; DeVe: decidual vessel. Original magnification 40x. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2013/689768/) licensed under a CC-BY license. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0015.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Brain, Cerebral Cortex, Cell Processes in Gray Matter 40x.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0008.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Analysis in PFA fixed NIH/3T3 cells using anti-LC3A antibody. Image from verified customer review. LC3A Antibody [NB100-2331]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0012.jpg)
![Immunocytochemistry/ Immunofluorescence: LC3 Antibody Pack [NB910-40435] - The autophagy level was increased in degenerated mouse SGNs. (a-c) Western blot results revealed that the levels of the autophagy-related proteins LC3 & BECN1 were increased in the degenerated SGNs on the 5th, 15th & 30th day after ototoxic drug administration & were significantly different from those in the normal mice. *, P < 0.05. (d) Immunofluorescence staining of LC3 puncta (red) also demonstrated that the LC3 level in the degenerated SGNs (green) was significantly increased on the 30th day after drug administration. Con, normal mice without drug treatment; 30D, 30 days after drug administration. Images of immunofluorescence staining were taken from the middle turn of cochlea. Scale bar: 10 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb910-40435_lc3-antibody-pack-31020241538249.jpg)
![Western Blot: LC3 Antibody Pack [NB910-40435] - The autophagy level was increased in degenerated mouse SGNs. (a-c) Western blot results revealed that the levels of the autophagy-related proteins LC3 & BECN1 were increased in the degenerated SGNs on the 5th, 15th & 30th day after ototoxic drug administration & were significantly different from those in the normal mice. *, P < 0.05. (d) Immunofluorescence staining of LC3 puncta (red) also demonstrated that the LC3 level in the degenerated SGNs (green) was significantly increased on the 30th day after drug administration. Con, normal mice without drug treatment; 30D, 30 days after drug administration. Images of immunofluorescence staining were taken from the middle turn of cochlea. Scale bar: 10 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb910-40435_lc3-antibody-pack-310202415284514.jpg)
![Western Blot: LC3 Antibody Pack [NB910-40435] - CCI-779 significantly rescued the impaired autophagy-lysosomal pathway in degenerated SGNs of mice. (a) The levels of Ctsb, Ctsd, & Lamp1 & of the autophagic genes Becn1 & Lc3b were significantly higher, & Sqstm1 was lower in the experimental group than in the negative control group, which was determined by quantitative real-time PCR. (b & c) The LAMP1 & CTSD levels determined by western blotting were consistent with the quantitative real-time PCR results. (d & e) Compared with those in the negative control group, the LC3 & BECN1 levels in the experimental groups were significantly increased, as determined by western blot assays. (f & g) The western blot results revealed that the levels of the autophagic cargo receptor SQSTM1 & ubiquitinated proteins were decreased significantly in the experimental group compared with those in the negative control groups. *, the difference between the experimental group & the blank control group was significant (P < 0.05); #, the difference between the experimental group & the negative control group was significant (P < 0.05); CCI-779, experimental group; 30D, negative control group; Con, blank control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb910-40435_lc3-antibody-pack-310202416163727.jpg)
![Western Blot: USP7 Antibody [NB100-513] - Samples: Wholecell lysate (10 ug) from HEK293T, RKO, Hep-G2, Jurkat,HeLa, TCMK-1, and NIH 3T3 cells prepared using NETNlysis buffer. Antibody: Affinity purified rabbit anti-USP7antibody used for WB at0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 10 seconds](http://images.novusbio.com/fullsize/USP7-Antibody-Western-Blot-NB100-513-img0004.jpg "Western Blot: USP7 Antibody [NB100-513] - Samples: Wholecell lysate (10 ug) from HEK293T, RKO, Hep-G2, Jurkat,HeLa, TCMK-1, and NIH 3T3 cells prepared using NETNlysis buffer. Antibody: Affinity purified rabbit anti-USP7antibody used for WB at0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 10 seconds")
![Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - Sample: FFPE section of human ovarian carcinoma.Antibody: Affinity purified rabbit anti-USP7 used at 1:5,000 (0.2ug/ml). Detection:DAB](http://images.novusbio.com/fullsize/USP7-Antibody-Immunohistochemistry-Paraffin-NB100-513-img0002.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - Sample: FFPE section of mouse renal cell carcinoma.Antibody: Affinity purified rabbit anti-USP7 used at 1:5,000 (0.2ug/ml). Detection:DAB](http://images.novusbio.com/fullsize/USP7-Antibody-Immunohistochemistry-Paraffin-NB100-513-img0001.jpg)
![Immunoprecipitation: USP7 Antibody [NB100-513] - Samples: Wholecell lysate (10 ug) from HEK293T, RKO, Hep-G2, Jurkat,HeLa, TCMK-1, and NIH 3T3 cells prepared using NETNlysis buffer. Antibody: Affinity purified rabbit anti-USP7antibody used for WB at0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 10 seconds](http://images.novusbio.com/fullsize/USP7-Antibody-Immunoprecipitation-NB100-513-img0003.jpg)
![Immunohistochemistry: CD11b Antibody (M1/70.15) [NB600-1327] - Immunohistochemical analysis of frozen lymph node section using CD11b Antibody (M1/70.15) [NB600-1327] followed by staining with HRP-conjugated secondary antibody.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunohistochemistry-NB600-1327-img0012.jpg "Immunohistochemistry: CD11b Antibody (M1/70.15) [NB600-1327] - Immunohistochemical analysis of frozen lymph node section using CD11b Antibody (M1/70.15) [NB600-1327] followed by staining with HRP-conjugated secondary antibody.")
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Characterization of microglia cultures by multiple staining with CD11b Antibody (M1/70.15) [NB600-1327] (panels a. & c.) and anti GFAP (panels b & d). Nuclei appear blue due to 4',6-diamidino-2-phenylindole (DAPI) counter staining.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunocytochemistry-Immunofluorescence-NB600-1327-img0013.jpg)
![Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - A surface stain was performed on Raw264.7 cells with CD11b (M1/70.15) Antibody [NB600-1327R] (blue) and a matched isotype control (orange), both conjugated to DyLight 550. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Flow-Cytometry-NB600-1327-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] (NB600-1327) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-1327-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] conjugated to Alexa Fluor 488 (NB600-1327AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-1327-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemistry/Immunofluorescence (ICC/IF) analysis of RAW264.7 cells using CD11b antibody (M1/70.15) [NB600-1327] with Dylight 488 conjugated secondary (green). Nuclei and tubulin were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunocytochemistry-Immunofluorescence-NB600-1327-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemical/Immunofluorescent staining in untreated and treated mouse microglia cells using CD11b Antibody (M1/70.15) [NB600-1327]. Image from a verified customer review.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunocytochemistry-Immunofluorescence-NB600-1327-img0015.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemical/Immunofluorescent analysis in mouse primary microglia. Cells were treated with lysophosphatidic acid (LPA) or LPA and an LPAR5 inhibitor in order to investigate the expression of iNOS after 24 hours. CD11b Antibody (M1/70.15) [NB600-1327] was used in a dilution 1:100 in order to stain primary murine microglia. Cy2 (green) and Cy3 (red) were used as secondary antibodies.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunocytochemistry-Immunofluorescence-NB600-1327-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] conjugated to Alexa Fluor 647 (NB600-1327AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-1327-img0019.jpg)
![Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327].](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Flow-Cytometry-NB600-1327-img0004.jpg)
![Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327]: RPE - Alexa Fluor 750](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Flow-Cytometry-NB600-1327-img0007.jpg)
![Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages using the Alexa Fluor (R) 488 conjugate of CD11b Antibody (M1/70.15) [NB600-1327AF488].](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Flow-Cytometry-NB600-1327-img0014.jpg)
