Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-442SS | |||
| Azide and BSA Free | NBP2-80727 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-644SS | |||
| Azide and BSA Free | NBP2-80970 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7343APC | |||
| Biotin | NB7343B | |||
| DyLight 350 | NB7343UV | |||
| DyLight 405 | NB7343V | |||
| DyLight 488 | NB7343G | |||
| DyLight 550 | NB7343R | |||
| DyLight 594 | NB7343DL594 | |||
| DyLight 650 | NB7343C | |||
| DyLight 680 | NB7343FR | |||
| DyLight 755 | NB7343IR | |||
| FITC | NB7345 | |||
| HRP | NB7346 | |||
| Janelia Fluor 525 | NB7343JF525 | |||
| Janelia Fluor 669 | NB7343JF669 | |||
| PE | NB7343PE | |||
| PerCP | NB7343PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1118-SP | |||
![Flow Cytometry: Ethenoadenosine Antibody (1G4) [NB600-442] - Chicken erythrocytes (1% v/v), isolated by density gradient centrifugation, were transiently transfected with a Flag-tagged chicken ART4 containing plasmid or the empty plasmid (pSecTag B), and incubated at 37C in the presence or absence of 200 uM etheno-NAD+ for 30 min. After washing, cells were stained with an etheno-adenosine specific antibody (1G4) or an anti-Flag antibody and the respective isotype control stained with the 1G4 antibody. Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-86), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ethenoadenosine-Antibody-1G4-Flow-Cytometry-NB600-442-img0002.jpg "Flow Cytometry: Ethenoadenosine Antibody (1G4) [NB600-442] - Chicken erythrocytes (1% v/v), isolated by density gradient centrifugation, were transiently transfected with a Flag-tagged chicken ART4 containing plasmid or the empty plasmid (pSecTag B), and incubated at 37C in the presence or absence of 200 uM etheno-NAD+ for 30 min. After washing, cells were stained with an etheno-adenosine specific antibody (1G4) or an anti-Flag antibody and the respective isotype control stained with the 1G4 antibody. Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-86), licensed under a CC-BY license.")
![Flow Cytometry: Ethenoadenosine Antibody (1G4) [NB600-442] - Detection of ADP-ribosylated proteins by flow cytometry. C-33A cells (1 x 10^7/mL), transiently transfected with a Flag-tagged chicken ART4 containing plasmid, were incubated at 37C in the presence or absence of 200 uM etheno-NAD+ for 30 min. After washing, cells were stained with an etheno-adenosine specific antibody (1G4). Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-86), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ethenoadenosine-Antibody-1G4-Flow-Cytometry-NB600-442-img0001.jpg)
![Flow Cytometry: Ethenoadenosine Antibody (1G4) [NB600-442] - Etheno-ADP-ribosylation of cell-surface proteins on C3H-10T1/2 cells. C3H-10T 1/2 cells at different stages of differentiation (progenitors, MyoD-myoblasts, myotubes) were incubated for 30 minutes in the absence (dashed lines) or presence (solid lines) of 100 uM etheno-NAD. Cells were then washed, incubated with the primary monoclonal antibody 1G4, stained with a FITC-conjugated goat-anti-mouse secondary antibody and subjected to FACS analysis. Data show one representative experiments out of three. Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-91), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ethenoadenosine-Antibody-1G4-Flow-Cytometry-NB600-442-img0003.jpg)
![N/A IL-18 BPa/IL18BP [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL18_BPa_DY119_ELISA_1598.jpg "N/A IL-18 BPa/IL18BP [Biotin]")
![Immunohistochemistry: Survivin Antibody (8E2) [NB500-644] - Immunohistochemical analysis of Survivin in human breast cancer tissue using Survivin Antibody (8E2) [NB500-644] and DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Immunohistochemistry-NB500-644-img0005.jpg "Immunohistochemistry: Survivin Antibody (8E2) [NB500-644] - Immunohistochemical analysis of Survivin in human breast cancer tissue using Survivin Antibody (8E2) [NB500-644] and DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: Survivin Antibody (8E2) [NB500-644] - Immunocytochemical analysis using Survivin Antibody (8E2) [NB500-644] was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Immunocytochemistry-Immunofluorescence-NB500-644-img0004.jpg)
![Flow (Intracellular): Survivin Antibody (8E2) [NB500-644] - An intracellular stain was performed on Daudi cells with Survivin Antibody (8E2) [NB500-644] (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Flow-Intracellular-NB500-644-img0006.jpg)
![N/A P-Selectin/CD62P [Biotin]](http://images.novusbio.com/fullsize2/DATA_PSelectin_DY137_ELISA_1636.jpg "N/A P-Selectin/CD62P [Biotin]")
![N/A Fetuin A/AHSG [HRP]](http://images.novusbio.com/fullsize2/DATA_Fetuin_A_DFTA00_ELISA_684.jpg "N/A Fetuin A/AHSG [HRP]")
![N/A Fetuin A/AHSG [HRP]](http://images.novusbio.com/fullsize2/DATA_Fetuin_A_DFTA00_ELISA_685.jpg)
![N/A Fetuin A/AHSG [HRP]](http://images.novusbio.com/fullsize2/Fetuin_A_DFTA00_ELISA_99.jpg)
![N/A IL-10 [HRP]](http://images.novusbio.com/fullsize2/DATA_IL10_P1000_ELISA_1053.jpg "N/A IL-10 [HRP]")
![N/A IL-10 [HRP]](http://images.novusbio.com/fullsize2/IL-10_P1000_ELISA_339.jpg)
![N/A S100A8/S100A9 Heterodimer [Biotin]](http://images.novusbio.com/fullsize2/DATA_S100A8_S100A9_Heterodimer_DY822605_ELISA_2535.jpg "N/A S100A8/S100A9 Heterodimer [Biotin]")
![N/A Progranulin/PGRN [HRP]](http://images.novusbio.com/fullsize2/DATA_Progranulin_DPGRN0_ELISA_790.jpg "N/A Progranulin/PGRN [HRP]")
![N/A Progranulin/PGRN [HRP]](http://images.novusbio.com/fullsize2/DATA_Progranulin_DPGRN0_ELISA_791.jpg)
![N/A Progranulin/PGRN [HRP]](http://images.novusbio.com/fullsize2/Progranulin_DPGRN0_ELISA_159.jpg)
![ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343] - Sandwich ELISA for total dog IgE measurement showing standard curve at 2 different coating antibody concentrations. Coating: 1ug/ml in carbonate buffer, pH 9.6. Standard: purified dog IgE, range: 156pg/ml to 15.000 pg/ml. Detection with secondary antibody (HRP-labeled) and TMB substrate. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Goat-anti-Canine-IgE-Secondary-Antibody-ELISA-NB7343-img0001.jpg "ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343] - Sandwich ELISA for total dog IgE measurement showing standard curve at 2 different coating antibody concentrations. Coating: 1ug/ml in carbonate buffer, pH 9.6. Standard: purified dog IgE, range: 156pg/ml to 15.000 pg/ml. Detection with secondary antibody (HRP-labeled) and TMB substrate. This image was submitted via customer Review.")
![Dot Blot: Goat anti-Canine IgE Secondary Antibody [NB7343] - D.p. extracts have effects on MCs though the IgE-FcepsilonRI axis. SDS-PAGE of recombinants (300 ng) and Western blotting (168.8 ng, for rDer p 15; 143.8 ng of rDer p 18 per gel lane) of specific IgE in canine sera. Image collected and cropped by CiteAb from the following publication (//waojournal.biomedcentral.com/articles/10.1186/s40413-017-0154-3), licensed under a CC-BY license.. Using the HRP format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Canine-IgE-Secondary-Antibody-Dot-Blot-NB7343-img0002.jpg)
![N/A Dkk-1 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Dkk1_DY1906_ELISA_1733.jpg "N/A Dkk-1 [Biotin]")
![N/A CXCL4/PF4 [HRP]](http://images.novusbio.com/fullsize2/DATA_CXCL4_DPF40_ELISA_787.jpg "N/A CXCL4/PF4 [HRP]")
![N/A CXCL4/PF4 [HRP]](http://images.novusbio.com/fullsize2/CXCL4_DPF40_ELISA_473.jpg)