Antibody database

The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evaded. The MHC complex is broken into two categories, MHC Class I proteins and MHC Class II proteins.  MHC complex I and II proteins are all very different and contain specific molecules to bind different peptides – in fact, they have been described as the most polymorphic genes there are.

Novus employees will be participating in the March for Science alongside a global network of scientists to support the education and public importance of this topic for all.

We believe in the integrity of science and all that it holds for the future. Everyone is affected by it, and we believe in sharing this information with the public, outside of labs and journals.

Join us on April 22, 2017 at the March in D.C. or at your local satellite event. #BioTechneMarches

We will be marching in our Bio-Techne communities:

c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B.  Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D.  Each member of the AP-1 transcription family is a phosphonuclear protein composed of a carboxy-terminal leucine zipper domai

A tissue microarray is a fairly recent high-throughput application that allows researchers to test hundreds of tissue samples with antibodies of their choice at once.  Essentially, a tissue microarray is a paraffin block that is produced by a composition of tissue cores from paraffin donor blocks within defined coordinates to account for a variety of tissue types.

Immunocytochemistry (ICC) can be a very effective method for visualizing the localization and behavior of intracellular proteins, however the protocol for each ICC experiment should be optimized specifically to the cell being examined.  Permeabilization, or the puncturing of the cell membrane, is an extremely important step in detecting intracellular antigens with a primary antibody because it allows entry through the cell membrane. Permeabilization is introduced after cells have been prepared with a fixative agent to initiate protein cross-linking, such as formaldehyde or ethanol.

Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variety of antibodies and tools to detect the different morphological indicators of cell death. 

Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and some of their key regulatory targets are discussed below.

Apoptosis

Apoptosis, programmed cell death, is primarily characterized by the activation of caspases which further regulate the mass cleavage of proteins and DNA. Some of major the proteins responsible for various apoptotic events are:

Primary antibodies are becoming increasingly popular investigate tool to use in research, given the breadth and selection of product on the market. Not only are antibodies raised in a variety of hosts, they also have proven reactivity with a wide range of species applying to many research fields and models.

Mammalian target of Rapamycin (mTOR) is a serine/threonine kinase that regulates cell growth, proliferation, motility and survival.

Apoptosis is a form of programmed cell death which is mediated by cysteine proteases called caspases. It is an essential phenomenon in the maintenance of homeostasis and growth of tissues, and it also plays a critical role in immune response. The cytomorphological alterations and the key features of apoptosis are listed below: