Antibodies

Apurinic/apyrimidinic (AP) endonuclease 1 (APE1) plays an important role in the DNA base excision repair pathway.

Nuclear Mitotic Apparatus protein (NuMA) is a cell cycle-related protein that acts as an organizer of the mitotic spindle during mitosis. It may be involved in coordinating the alignment of the mitotic spindle to the cellular polarity axis, which is a prerequisite for asymmetric cell division. NuMA is also a prominent component of interphase cell nuclear matrix; however its role during interphase is largely unknown.

Centromere protein F (CENPF) also known as Mitosin, AH antigen, and kinetochore protein CENPF, is a protein that associates with the centromere-kinetochore complex. CENPF forms both a homodimer and a heterodimer. CENPF can be found in different cellular locations depending on the stage of mitosis.

The availability of commercial antibodies for a myriad of scientific applications has had a profound positive impact on life science research. It's not surprising that the global antibody market, including therapeutics, diagnostics, and research, is now estimated at $70-75 billion.

And yet, almost half of 400 antibody users surveyed by 1DegreeBio in 2012 reported that that in 50% of cases, their antibodies did not work as expected. That’s a lot of failed experiments—and a lot of money wasted.

NeuN is a RNA-binding protein that modulates alternative splicing and is localized both to the nucleus and cytoplasm. It is a member of the RNA-binding FOX (RBFOX) family of splicing regulators which includes RBFOX1 (Fox-1/A2BP1) and RBFOX2 (Fox-2/RBM9). Each FOX family member is differentially expressed – RBFOX1 in neurons, heart, and muscle while RBFOX3 is exclusively limited to neurons.

Synaptonemal Complex Protein 3 (SCP3), which is a protein present in the synaptonemal complex which is responsible for pairing, synapsis, and recombination of homologous chromosomes during meiosis. Meiosis, in basic terms, is where germ cells divide to produce gametes. This is accomplished through DNA replication and two rounds of cell division.

NBS1 (Nijmegen breakage syndrome protein 1) is a component of the MRN complex (Mre11-Rad50-Nbs1) that plays important role in detecting DNA double strand breaks (DSBs) and triggering the downstream cascade. DSBs can be caused by ionizing radiation, chemotherapy drugs, metabolic ROS, replication errors, programmed enzymatic activities during meiosis/V(D)J recombination, etc.

xCT is an obligate, electroneutral, membrane-bound anionic transporter responsible for regulating particular amino acid gradients via their transport through plasma membrane. The antiporter xCT superficially resembles an ion channel and preferentially catalyzes the exchange of L-cystine for L-glutamate residues in animal cells. Unlike other glutamate transporters like EAATs, xCT functions independently of an electrochemical sodium ion gradient. xCT is present in most peripheral tissues (heart, bone, liver, and testes).

The low density lipoprotein receptor coordinates the metabolism of cholesterol, an essential component of the mammalian cell plasma membranes. Study of this carefully balanced system has led to an enhanced understanding of cholesterol homeostasis at the cellular level. Receptor-mediated endocytosis (RME) is an important mechanism of metabolic regulation.

Caveolin-1 (CAV1) belongs to the caveolin family of integral membrane proteins 21-24 kD in size. This family of proteins forms the structural component of the caveolar membrane in caveolae, which are the specialized domains in plasma membrane that sequester lipids and proteins.