Immunoprecipitation and Western blot. The investigator should determine the optimal working dilution for a specific application. Other applications have not yet been determined. Previous customers have reported poor signal in ELISA against the Abeta peptide.
Publications
Read Publication using NB300-226 in the following applications:
NB 300-226 reacts with human and mouse forms of the protein. There is minimal cross-reactivity with Abeta 40 (see Notes).
Packaging, Storage & Formulations
Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified
Alternate Names for Abeta 42 Antibody
Amyloid beta 42
Beta-amyloid protein 42
Background
Amyloid beta-protein (Abeta) is associated with neuronal injury and death in Alzheimer's disease. These proteins are the main components of amyloid plaques. Abeta 42 plays a bigger role in deneurogeneration and is more strongly associated with Alzheimer's disease than Abeta 40.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Abeta 42 Antibody (NB300-226). (Showing 1 - 3 of 3 FAQ).
Our customer wants to detect by Western Blot the Amyloid beta 42 in mouse brain homogenates. They saw your antibody Anti-Amyloid beta 42 Polyclonal Antibody (NB300-226). They would like to be sure that it can uses in Western Blot on wild type mice (not transgenic mice expressing human genes). Can you advise.
Reactivity with mouse was reported by customer feedback, and unfortunately I am not 100% certain whether they used wild type mice or a different strain engineered to express the human protein. Regardless, I will guarantee this antibody for you 100% in murine samples using western blot and IP, and we do sell a sample size of this product if you would like to test a smaller amount before committing to a full-size vial. (We determine species reactivity based on the antibodies abilitiy to detect the species specific protein. We fully guarantee all listed species for our products.)
Our customer is interest in this antibody NB300-226. And he also would like to order the Synthetic peptide of this item, is the synthetic peptide available for this antibody?
Unfortunately we do not have the blocking peptide available for this item. We do not have any IHC images available for NBP1-20267 and it has not been validated or tested for use in Immunofluorescence.
I would like to ask you if I could use this antibody (NB300-226) to detect the Amyloid 1-42 monomer and not polymer?
I have no information as to whether this antibody will detect monomer only. As a polyclonal antibody, it will almost certainly detect both. I think your best bet to find a monomer specific antibody would be to use a monoclonal.
Using Amyloid beta peptides in Alzheimer's Disease Immunization Amyloid beta (AB) peptide has a central role in the neurodegeneration of Alzheimer's disease (AD). Immunization of AD transgenic mice with AB-42 peptide reduces both the spatial memory impairments and AD-like neuropathologic changes.Therapeutic im... Read full blog post.
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