| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73979 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49537SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34760-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56368SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43359-0.05mg | |||
| BSA Free | NBP1-43359-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NBP1-28035 | |||
| Biotin | NBP1-28033-500ug | |||
| FITC | NBP1-28032 | |||
| PE | NBP1-28034 | |||
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0023.jpg "Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract")
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - ARPE-19 monolayer on glass substrate. Fixative Methanol Blocking Goat Serum. Dilution 1:100. Incubation 1h @ RT. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-NB300-750-img0024.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of rat whole eye extract](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0017.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of various human, mouse and non-human primate whole cell lysates.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0016.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of extracts from HeLa, U251 and mouse brain using PAX6 antibody (NB300-750, 1:100).](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0019.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of Pax6 in skate (Leucoraja erinacea) brain lysate using anti-Pax6 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NB300-750-img0011.jpg)
![Immunoprecipitation: Pax6 Antibody [NB300-750] - Analysis of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500ug whole cell lysate with 5ug of PAX6 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:1000 for at least one hour.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunoprecipitation-NB300-750-img0020.jpg)
![Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NB300-750] - Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs).(a) Schematic description of the two-stage protocol applied to iPSC-renal commitment. (b, c, d, e) Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. (b) The pluripotency markers SSEA4, TRA-1-81, Nanog, ME marker T(Bry) & IM marker Osr1. (c) IM & MM marker expression as Wt1, Pax8, Pax2, Six2 & Sall1. (d) Renal progenitor markers CD133, CD24, NCAM, glomerular epithelial marker Claudin1 & proximal tubular epithelial markers AQP1, GGT1. (e) Markers identifying endodermal AFP, ectodermal Pax6, & cardiac mesodermal Nkx2.5 derivation. Nuclei are stained with DAPI (blue). Scale bars: 20 μm (b, c, d, e). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-750_rabbit-polyclonal-pax6-antibody-3102024161898.jpg)
![Western Blot: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Western-Blot-NBP2-03285-img0020.jpg "Western Blot: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunocytochemistry-Immunofluorescence-NBP2-03285-img0019.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Human tonsil using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0018.jpg)
![Flow Cytometry: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of Jurkat cells, using anti-SAMHD1 antibody,(Red), compared to a nonspecific negative control antibody,(Blue).](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Flow-Cytometry-NBP2-03285-img0022.jpg)
![Western Blot: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Western-Blot-NBP2-03285-img0012.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Adenocarcinoma of Human endometrium tissue using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0014.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Carcinoma of Human liver tissue using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0015.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Human lymphoma tissue using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0016.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Human pancreas tissue using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0017.jpg)
![Flow Cytometry: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of Hela cells, using anti-SAMHD1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Flow-Cytometry-NBP2-03285-img0011.jpg)
![Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Western-Blot-NB100-59828-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Cdc20-Antibody-Immunohistochemistry-NB100-59828-img0010.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human CDC20 on HeLa whole cell lysate using NB100-59828. CDC20 was also immunoprecipitated by rabbit anti-CDC20 antibody NB100-59827.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Western-Blot-NB100-59828-img0006.jpg)
![Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Immunohistochemistry-NB100-59828-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Cdc20 Antibody [NB100-59828] - Detection of human CDC20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC20 antibody NB100-59828 (lot NB100-59828-2) used for IP at 6 ug per reaction. CDC20 was also immunoprecipitated by a previous lot of this antibody (lot NB100-59828-1) and rabbit anti-CDC20 antibody NB100-59827. For blotting immunoprecipitated CDC20, NB100-59828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Immunoprecipitation-NB100-59828-img0013.jpg)
![Western Blot: LIN-28A Antibody [NBP1-49537] - Hypoxia promoted an increase in the expression of SOX-2, OCT-4, KLF-4, Nanog, Lin-28A and CD133. The RT-qPCR analysis showed an upregulation in the expression of Lin-28A in a time-dependent manner in sorted A549 cells under hypoxic conditions (*P<0.05). (b) Western blot analysis showed that the transcription factors and stem cell markers were not expressed under normoxia. However, there was an increase in the expression of SOX-2 (MAB2018), OCT-4 (MAB1759), KLF-4 (AF3640), Nanog (AF1997), Lin-28A and CD133 in a time-dependent manner after hypoxia treatment in sorted A549 cells. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddiscovery2016105) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Western-Blot-NBP1-49537-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: LIN-28A Antibody [NBP1-49537] - Hypoxia promoted an increase in the expression of SOX-2, OCT-4, KLF-4, Nanog, Lin-28A and CD133. The RT-qPCR analysis showed an upregulation in the expression of Lin-28A in a time-dependent manner in sorted A549 cells under hypoxic conditions (*P<0.05). (b) Western blot analysis showed that the transcription factors and stem cell markers were not expressed under normoxia. However, there was an increase in the expression of SOX-2 (MAB2018), OCT-4 (MAB1759), KLF-4 (AF3640), Nanog (AF1997), Lin-28A and CD133 in a time-dependent manner after hypoxia treatment in sorted A549 cells. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddiscovery2016105) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LIN-28A Antibody [NBP1-49537] - (e) Immunofluorescence staining showed that A549 CD133-negative cells highly expressed Lin-28A after 48 h of 1% O2 exposure. (k) Immunofluorescence staining showed that HepG2 CD133-negative cells highly expressed Lin-28A after 48 h of 1% O2 exposure.Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddiscovery2016105) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49537-img0015.jpg)
![Immunohistochemistry: LIN-28A Antibody [NBP1-49537] - Analysis of LIN28 in E17.5 mouse embryo.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunohistochemistry-NBP1-49537-img0011.jpg)
![Western Blot: LIN-28A Antibody [NBP1-49537] - Analysis of LIN28 in NTERA-2 cell lysate.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Western-Blot-NBP1-49537-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody [NBP1-49537] - Analysis of LIN28 in NTERA-2 cells using NBP1-49537. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49537-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody [NBP1-49537] - Immunofluorescence staining showed that the newly formed GL261 spheres highly expressed Lin-28A. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddiscovery2016105) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49537-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunocytochemistry-Immunofluorescence-NBP2-32808-img0009.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Staining of paraffin-embedded Leiomyosarcoma using the Azide and BSA-Free version of alpha-Smooth Muscle Actin (SPM332) Antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunohistochemistry-Paraffin-NBP2-32808-img0004.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0008.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP. Image from the PerCP version of this antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0006.jpg)
![Western Blot: Noxa Antibody [NB100-56368] - Noxa expression was stably reduced in HCT116 cells by shRNA. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Noxa-Antibody-Western-Blot-NB100-56368-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Noxa Antibody [NB100-56368] - Noxa expression was stably reduced in HCT116 cells by shRNA. Western blot image submitted by a verified customer review.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Noxa Antibody [NB100-56368] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using Noxa antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Noxa-Antibody-Immunohistochemistry-Paraffin-NB100-56368-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Noxa Antibody [NB100-56368] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Noxa (NB100-56368) antibody at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Noxa-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56368-img0003.jpg)
![Western Blot: Noxa Antibody [NB100-56368] - Western blot analysis of Noxa in A) human, B) mouse, and C) rat thymus tissue using Noxa antibody NB100-56368 at 2 ug/mL.](http://images.novusbio.com/fullsize/Noxa-Antibody-Western-Blot-NB100-56368-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Scale bar= 10um.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32791-img0022.jpg "Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Scale bar= 10um.")
![Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Rat brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-Immunohistochemistry-Paraffin-NBP1-32791-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody ) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32791-img0012.jpg)
![Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Mouse brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-Immunohistochemistry-Paraffin-NBP1-32791-img0023.jpg)
![Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Mouse brain.O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-Immunohistochemistry-Paraffin-NBP1-32791-img0026.jpg)
![Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Mouse brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-Immunohistochemistry-Paraffin-NBP1-32791-img0027.jpg)
![Immunoprecipitation: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Immunoprecipitation of O-GlcNAc transferase protein from A431 whole cell extracts using 5 ug of O-GlcNAc transferase antibody. Western blot analysis was performed using O-GlcNAc transferase antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-Immunoprecipitation-NBP1-32791-img0021.jpg)
![Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - O-GlcNAc Transferase p110 subunit antibody detects O-GlcNAc Transferase p110 subunit protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.O-GlcNAc Transferase p110 subunit stained by O-GlcNAc Transferase p110 subunit antibody (NBP1-32791) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32791_rabbit-polyclonal-o-glcnac-transferase-p110-subunit-antibody-910202420115049.jpg)
![Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - O-GlcNAc Transferase p110 subunit antibody detects O-GlcNAc Transferase p110 subunit protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.O-GlcNAc Transferase p110 subunit stained by O-GlcNAc Transferase p110 subunit antibody (NBP1-32791) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32791_rabbit-polyclonal-o-glcnac-transferase-p110-subunit-antibody-9102024205632.jpg)
![Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - O-GlcNAc Transferase p110 subunit antibody detects O-GlcNAc Transferase p110 subunit protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.O-GlcNAc Transferase p110 subunit stained by O-GlcNAc Transferase p110 subunit antibody (NBP1-32791) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32791_rabbit-polyclonal-o-glcnac-transferase-p110-subunit-antibody-91020242091461.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-32791_rabbit-polyclonal-o-glcnac-transferase-p110-subunit-antibody-1610202419414580.jpg)
![Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0046.jpg "<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PCNA Antibody [NB100-456] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- PCNA used at a dilution of 1:10,000 (0.1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-NB100-456-img0044.jpg)
![Flow Cytometry: PCNA Antibody [NB100-456] - Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X106 cells were stained with 0.03 ug anti-PCNA . Secondary detection was performed with FITC conjugated Goat F(ab')2 anti-rabbit antibody], and DNA stained with PI.](http://images.novusbio.com/fullsize/PCNA-Antibody-Flow-Cytometry-NB100-456-img0004.jpg)
![Western Blot: PCNA Antibody [NB100-456] - A431 whole cell lysates](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0041.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Analysis using the Biotin conjugate of NB100-456. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0043.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PCNA Antibody [NB100-456] - Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 ug for IP; 30 ug for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody NB100-456 used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using another a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). immunoprecipitatesd PCNA was blotted using NB100-456 (1 ug/ml). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunoprecipitation-NB100-456-img0047.jpg)
![Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Fluorescent images from representative ileum slices show putative neuronal cells and interstitial cells of Cajal. Immunoreactive c-Kit was localized by using the ACK2 antibody in live tissue after 48 h ex vivo. Scale bars are 25 um. Image collected and cropped by CiteAb from the following publication (//www.physiology.org/doi/10.1152/ajpgi.00299.2015) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Immunohistochemistry-Whole-Mount-NBP1-43359-img0003.jpg "Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Fluorescent images from representative ileum slices show putative neuronal cells and interstitial cells of Cajal. Immunoreactive c-Kit was localized by using the ACK2 antibody in live tissue after 48 h ex vivo. Scale bars are 25 um. Image collected and cropped by CiteAb from the following publication (//www.physiology.org/doi/10.1152/ajpgi.00299.2015) licensed under a CC-BY license.")
![Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of Allophycocyanin conjugate of NBP1-43359. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b kappa Isotype Control APC (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) APC (right). Total viable c](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Flow-Cytometry-NBP1-43359-img0001.jpg)
![Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of PE/Cy5 conjugate of NBP1-43359. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b Isotype Control PE-Cy5 (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) PE-Cy5 (right). Total viable cells were](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Flow-Cytometry-NBP1-43359-img0002.jpg)
![Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - Western-blots for a selection of differentially-expressed proteins. MyH2b, Troponin I Type 1 (slow skeletal) (TNNI1) and MCK were used as target protein; GADPH was used as a control. Total protein from control and arginine-supplemented pig samples was fractionated by SDS-PAGE, blotted and then detected with specific antibodies. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117294), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Troponin-I-Type-1-slow-skeletal-Antibody-Western-Blot-NBP1-56641-img0008.jpg "Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - Western-blots for a selection of differentially-expressed proteins. MyH2b, Troponin I Type 1 (slow skeletal) (TNNI1) and MCK were used as target protein; GADPH was used as a control. Total protein from control and arginine-supplemented pig samples was fractionated by SDS-PAGE, blotted and then detected with specific antibodies. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117294), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - Human Skeletal Muscle, 5.0ug/ml.](http://images.novusbio.com/fullsize/Troponin-I-Type-1-slow-skeletal-Antibody-Immunohistochemistry-Paraffin-NBP1-56641-img0006.jpg)
![Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/Troponin-I-Type-1-slow-skeletal-Antibody-Western-Blot-NBP1-56641-img0007.jpg)
![Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Troponin-I-Type-1-slow-skeletal-Antibody-Immunohistochemistry-Paraffin-NBP1-56641-img0005.jpg)
![Western Blot: APE Antibody [NB100-897] - Staining of A431 (A), HeLa (B) and MCF7 (C) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/APE-Antibody-Western-Blot-NB100-897-img0001.jpg "Western Blot: APE Antibody [NB100-897] - Staining of A431 (A), HeLa (B) and MCF7 (C) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: APE Antibody [NB100-897] - Staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/APE-Antibody-Immunohistochemistry-Paraffin-NB100-897-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (G7) [NBP1-28031] - Primary cortical neurons isolated from postnatal day 7 CD1 mice. Primary antibody incubation overnight at 1:500. Alexa Fluor secondary antibodies at 1:500. Image from a verified customer review.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-G7-Immunocytochemistry-Immunofluorescence-NBP1-28031-img0007.jpg "Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (G7) [NBP1-28031] - Primary cortical neurons isolated from postnatal day 7 CD1 mice. Primary antibody incubation overnight at 1:500. Alexa Fluor secondary antibodies at 1:500. Image from a verified customer review.")
![Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Flow Cytometry: CD90/Thy1 Antibody (G7) [Biotin] [NBP1-28033] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD90-BIOT and Rat Anti-Mouse CD3 epsilon-PE followed by Streptavidin-FITC. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-G7-Flow-Cytometry-NBP1-28031-img0006.jpg)
![Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Analysis using the Allophycocyanin conjugate of NBP1-28031. Multiple staining of BALB/c splenocytes.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-G7-Flow-Cytometry-NBP1-28031-img0002.jpg)
![Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Analysis using the Biotin conjugate of NBP1-28031. Multiple staining of BALB/c splenocytes.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-G7-Flow-Cytometry-NBP1-28031-img0003.jpg)
![Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Analysis using the FITC conjugate of NBP1-28031. Staining of 1 ug/1^6 BALB/c mesenteric lymph node cells with either rat IgG2c-FITC (as a negative control) or rat anti-mouse CD90-FITC.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-G7-Flow-Cytometry-NBP1-28031-img0004.jpg)
![Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Analysis using the PE conjugate of NBP1-28031. Multiple staining of BALB/c splenocytes.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-G7-Flow-Cytometry-NBP1-28031-img0005.jpg)
![CD90/Thy1 Antibody (G7) [NBP1-28031] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD90-UNLB followed by Mouse Anti-Rat IgG2c-FITC.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-G7-NBP1-28031-img0008.jpg)