| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70342 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74327 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23602SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49529SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75477SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB9167-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-59723SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-178SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77680SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB300-184-0.02ml | |||
![Western Blot: Catalase Antibody (1B8) [NBP2-00492] Analysis of extracts (35ug) from 9 different cell lines by using anti-Catalase monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Western-Blot-NBP2-00492-img0015.jpg "Western Blot: Catalase Antibody (1B8) [NBP2-00492] Analysis of extracts (35ug) from 9 different cell lines by using anti-Catalase monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Catalase Antibody (OTI1B8) [NBP2-00492] - Mouse hepatocytes stained with catalase antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Catalase-Antibody-OTI1B8-Immunocytochemistry-Immunofluorescence-NBP2-00492-img0019.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human lung tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0010.jpg)
![Flow Cytometry: Catalase Antibody (1B8) [NBP2-00492] - Analysis of Jurkat cells, using anti-Catalase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Flow-Cytometry-NBP2-00492-img0017.jpg)
![Western Blot: Catalase Antibody (1B8) [NBP2-00492] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Catalase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Catalase.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Western-Blot-NBP2-00492-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Catalase Antibody (1B8) [NBP2-00492] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Catalase.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunocytochemistry-Immunofluorescence-NBP2-00492-img0018.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0004.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0006.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human endometrium tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0007.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human liver tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0009.jpg)
![Flow Cytometry: Catalase Antibody (1B8) [NBP2-00492] - Analysis of Hela cells, using anti-Catalase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Flow-Cytometry-NBP2-00492-img0016.jpg)
![Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of extracts (35 ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Western-Blot-NBP2-01860-img0006.jpg "Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of extracts (35 ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Immunocytochemistry-Immunofluorescence-NBP2-01860-img0005.jpg)
![Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Jurkat cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Flow-Cytometry-NBP2-01860-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Loss of AFG3L2 and/or YME1L is associated with elevated OMA1, diminished SPG7, and a markedly altered pattern of OPA1 protein forms. Whole-cell lysates were resolved using SDS-PAGE and western blotted with antibody to Drp1, Oma1, and Spg7. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/19/12/3930), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Western-Blot-NBP2-01860-img0007.jpg)
![Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Western-Blot-NBP2-01860-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860] - Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Immunocytochemistry-Immunofluorescence-NBP2-01860-img0004.jpg)
![Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Hela cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Flow-Cytometry-NBP2-01860-img0001.jpg)
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [FITC] [NBP2-27229] - TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE this antibody and 1 ug of either isotype control (Left), or TLR4-FITC (right). PPI negative, CD14+ cells were gated for analysis. Image using the FITC form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0008.jpg "Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [FITC] [NBP2-27229] - TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE this antibody and 1 ug of either isotype control (Left), or TLR4-FITC (right). PPI negative, CD14+ cells were gated for analysis. Image using the FITC form of this antibody.")
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Analysis of Alexa Fluor (R) 488 conjugate of NBP2-27231. Cell surface 10^6 human PBMC using 1 ug of mouse isotype control. The shaded histogram represents human PBMC alone; green represents mouse isotype control. FITC-conjugated goat anti-mouse secondary](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0002.jpg)
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Cell surface analysis of 10^6 human PBMC using 1 ug of mouse isotype control. The shaded histogram represents human PBMC alone; green represents mouse isotype control . FITC-conjugated goat anti-mouse secondary antibody was used for this experiment.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0001.jpg)
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0003.jpg)
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 488] [NBP2-27231AF488] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0004.jpg)
![Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor (R) 647] [NBP2-27228] - Cell surface analysis of CD86 in human monocytes using 0.1 ug of NBP2-00308 conjugated to Alexa Fluor 647. The shaded histogram represents human monocytes alone; green represents mouse isotype control ; red represents CD86 this antibody. Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cell-Surface-NBP2-27231-img0005.jpg)
![Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 700] [NBP2-27231AF700] - A surface stain was performed on hPBMCs with CCR1 (53504) antibody FAB145N and a matched isotype control NBP2-27231AF700. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488. Image using the Alexa Fluor 700 form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cell-Surface-NBP2-27231-img0006.jpg)
![Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Allophycocyanin] [NBP2-27231APC] - A surface stain was performed on human peripheral blood monocytes with EMMPRIN/CD147 (MEM-M6/2) antibody NB500-561APC (blue) and a matched isotype control NBP2-27231APC (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cell-Surface-NBP2-27231-img0007.jpg)
![Western Blot: c-Fos Antibody [NB110-75039] - Western blot on marmoset brain lysate prepared in RIPA buffer containing 1% Triton-X100; 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:2000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NB110-75039-img0001.jpg "Western Blot: c-Fos Antibody [NB110-75039] - Western blot on marmoset brain lysate prepared in RIPA buffer containing 1% Triton-X100; 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:2000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC-P on paraffin sections of mouse brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NB110-75039-img0003.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC-P on paraffin sections of mouse brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NB110-75039-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23602-img0007.jpg "Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: GLI-2 Antibody [NBP2-23602] - GLI-2 (red) and DAPI (blue) in mouse BT-474 primary tumors (breast cancer tissue). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-Paraffin-NBP2-23602-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - GLI-2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:50 was used. Image objective 40x.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23602-img0004.jpg)
![Immunohistochemistry: GLI-2 Antibody [NBP2-23602] - Analysis of GLI-2 in mouse heart.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-NBP2-23602-img0005.jpg)
![Western Blot: ApoE4 Antibody (4E4) [NBP1-49529] - Analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529. Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.](http://images.novusbio.com/fullsize/ApoE4-Antibody-4E4-Western-Blot-NBP1-49529-img0004.jpg "Western Blot: ApoE4 Antibody (4E4) [NBP1-49529] - Analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529. Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.")
![Immunocytochemistry/Immunofluorescence: ApoE4 Antibody (4E4) [NBP1-49529] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ApoE4 (4E4) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ApoE4-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-49529-img0005.jpg)
![Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP1-75477-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP1-75477] - Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75477-img0006.jpg)
![Immunohistochemistry: Hsp27 Antibody [NBP1-75477] - Staining of HSP27 in mouse heart using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-NBP1-75477-img0007.jpg)
![Western Blot: Hsp27 Antibody [NBP1-75477] - Analysis of HSP27 in human skeletal muscle lysate.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0008.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167] - Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - Analysis of human bone marrow tissue lysate with 0.5 ug/ml of hELA-2 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Antibody. A specific band was detected for hELA-2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-950334-Western-Blot-MAB9167-img0001.jpg "Western Blot: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167] - Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - Analysis of human bone marrow tissue lysate with 0.5 ug/ml of hELA-2 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Antibody. A specific band was detected for hELA-2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Immunocytochemistry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free [MAB9167] - Neutrophil Elastase/ELA2 was detected in immersion fixed THP-1 cell using Neutrophil Elastase/ELA2 Monoclonal Antibody at 8 ug/ml dilution. Cells were stained using Donkey anti-Mouse IgG Secondary Antibody [NL557] (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-950334-Azide-Free-Immunocytochemistry-MAB9167-img0006.jpg)
![Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - ELA-2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using ELA-2 Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neutrophils .](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-950334-Immunohistochemistry-MAB9167-img0002.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free [MAB9167] - THP-1 human acute monocytic leukemia cell line treated with 3 uM monensin for 3 hours was stained with Mouse Anti-Human Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB9167, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-950334-Azide-Free-Flow-Cytometry-MAB9167-img0005.jpg)
![Western Blot: EGR2 Antibody [NB110-59723] - Detection of EGR2 in human fetal lung tissue using NB110-59723.](http://images.novusbio.com/fullsize/EGR2-Antibody-Western-Blot-NB110-59723-img0002.jpg "Western Blot: EGR2 Antibody [NB110-59723] - Detection of EGR2 in human fetal lung tissue using NB110-59723.")
![Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB110-59723] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-EGR2 Antibody NB110-59723 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-59723-img0007.jpg)
![Immunohistochemistry-Paraffin: EGR2 Antibody [NB110-59723] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of EGR2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunohistochemistry-Paraffin-NB110-59723-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB110-59723] - EGR2 staining on E13.5 dorsal root ganglion and neural tube.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunofluorescence-NB110-59723-img0006.jpg)
![Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.](http://images.novusbio.com/fullsize/NUMB-Antibody-Western-Blot-NB500-178-img0005.jpg "Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.")
![Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Numb-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: NUMB Antibody [NB500-178] - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Numb Antibody (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Numb-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0007.jpg)
![Simple Western: Numb Antibody [NB500-178] - Simple Western lane view shows a specific band for NUMB in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Numb-Antibody-Simple-Western-NB500-178-img0006.jpg)
![Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Western-Blot-NBP1-77680-img0009.jpg "Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.")
![Immunocytochemistry/Immunofluorescence: IGF-I R Antibody [NBP1-77680] - IGF1 receptor (EC) antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77680-img0008.jpg)
![Immunohistochemistry-Paraffin: IGF-I R Antibody [NBP1-77680] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney cancer xenograft using 1:200 dilution of IFG-I R antibody (NBP1-77680). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunohistochemistry-Paraffin-NBP1-77680-img0010.jpg)
![Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGF-I R in human pancreatic cancer cell lines (MiaPaCa-2, AsPC-1, and BxPC-3) using anti-IGF-I R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Western-Blot-NBP1-77680-img0007.jpg)
![Western Blot: CaMKII alpha [p Thr286] Antibody [NB300-184] - Anaylsis of Rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k beta-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/CaMKII-alpha-[p-Thr286]-Antibody-Western-Blot-NB300-184-img0003.jpg "Western Blot: CaMKII alpha [p Thr286] Antibody [NB300-184] - Anaylsis of Rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k beta-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")