Mouse IgG2b Isotype Control (MPC-11) [FITC]

Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [FITC] [NBP2-27229] - TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE this antibody and 1 ug of either isotype control (Left), or TLR4-FITC (right). PPI negative, CD14+ cells were gated for analysis.

• Reactivity: Mu
• Applications: Flow, IHC
• Clone: MPC-11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

Mouse IgG2b Isotype Control (MPC-11) [FITC] Summary

• Description: This Mouse IgG2B (MPC-11) immunoglobulin is useful as an isotype control for Mouse IgG2B (MPC-11) antibody.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 ul/1 million cells
  • Immunohistochemistry 1:10 - 1:500
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 50 mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Alternate Names for Mouse IgG2b Isotype Control (MPC-11) [FITC]

• IgG2B

Background

A mouse isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens. IMGENEX offers a variety of mouse isotype controls including fluorescene conjugates and azide-free formats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.