Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 647]

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Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor (R) 647] [NBP2-27228] - Cell surface analysis of CD86 in human monocytes using 0.1 ug of NBP2-00308 conjugated to Alexa Fluor 647. The shaded histogram ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
MPC-11
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647

Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 647] Summary

Description
This Mouse IgG2B (MPC-11) immunoglobulin is useful as an isotype control for Mouse IgG2B (MPC-11) antibody.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ul/1 million cells
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
Application Notes
Use the isotype control at the same concentration as the experimental antiobdy. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
50 mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Alternate Names for Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 647]

  • IgG2B

Background

A mouse isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens. IMGENEX offers a variety of mouse isotype controls including fluorescene conjugates and azide-free formats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Other Available Formats

Allophycocyanin Labeled NBP2-27231APC
Biotin Labeled NBP2-27231B
DyLight 350 Labeled NBP2-27231UV
DyLight 405 Labeled NBP2-27231V
DyLight 488 Labeled NBP2-27231G
DyLight 550 Labeled NBP2-27231R
DyLight 650 Labeled NBP2-27231C
DyLight 680 Labeled NBP2-27231FR
DyLight 755 Labeled NBP2-27231IR
FITC Labeled NBP2-27229
HRP Labeled NBP2-27231H
PE Labeled NBP2-27231PE
PerCP Labeled NBP2-27231PCP

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