Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 647]

Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor (R) 647] [NBP2-27228] - Cell surface analysis of CD86 in human monocytes using 0.1 ug of NBP2-00308 conjugated to Alexa Fluor 647. The shaded histogram represents human monocytes alone; green represents mouse isotype control ; red represents CD86 this antibody.

• Reactivity: Mu
• Applications: Flow, ICC/IF, IHC
• Clone: MPC-11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 647] Summary

• Description: This Mouse IgG2B (MPC-11) immunoglobulin is useful as an isotype control for Mouse IgG2B (MPC-11) antibody.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 ul/1 million cells
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 50 mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 647]

• IgG2B

Background

A mouse isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens. IMGENEX offers a variety of mouse isotype controls including fluorescene conjugates and azide-free formats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2b Isotype Control (NBP2-27228)(2)

Publications using NBP2-27228

• Thomas RC, Kheder R, Alaridhee H et al Complement Properdin Regulates the Metabolo-Inflammatory Response to a High Fat Diet Medicina (Kaunas) 2020-09-25 [PMID: 32971872] (FLOW, Mouse)
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Lee YL, Lin KL, Chuang SM et al. Elucidating Mechanisms of Bladder Repair after Hyaluronan Instillation in Ketamine-Induced Ulcerative Cystitis in Animal Model Am Am J Pathol. 2017-09-01 [PMID: 28826558] (IF/IHC, Rat)
  
  Details:
  Citation using the Alexa Fluor 647 form of this antibody.