| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1936SS | |||
| Azide and BSA Free | NBP2-80955 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24915SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-132SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90186-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44128 | |||
| BSA Free | NBP2-59061-25ul | |||
![Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. SMN proteins were found throughout the cytoplasm and as nuclear foci in control sensory neurons. SMN nuclear foci localized within CBs (coilin) in controls (upper panels, white arrows), whereas in the diabetic nucleus the numerous CBs were present but lost their colocalization with SMN nuclear foci (lower panels, yellow arrows). Arrowheads indicate sensory neurons magnified in the insets. Scale bars: 20 um, 10 um in insets. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/lookup/doi/10.1242/dmm.028225), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunohistochemistry-NB100-1936-img0007.jpg "Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. SMN proteins were found throughout the cytoplasm and as nuclear foci in control sensory neurons. SMN nuclear foci localized within CBs (coilin) in controls (upper panels, white arrows), whereas in the diabetic nucleus the numerous CBs were present but lost their colocalization with SMN nuclear foci (lower panels, yellow arrows). Arrowheads indicate sensory neurons magnified in the insets. Scale bars: 20 um, 10 um in insets. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/lookup/doi/10.1242/dmm.028225), licensed under a CC-BY license.")
![Western Blot: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN expression in 1) HeLa, 2) NTera2, 3) HepG2, 4) MCF7, 5) NIH 3T3, 6) PC12, and 7) COS7 whole cell lysates.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Western-Blot-NB100-1936-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB100-1936 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - scIF of control (f) and etv5b MO (g) cells from pmnx1:eGFP embryos. H2B, GFP and Smn signals are shown in Z-projected confocal sections. GFP+ cells marked by white rectangles are magnified on the right. Scale bars: 10 um for low and 5 um for high magnification. Image collected and cropped by Citeab from the following publication (Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Sci Rep (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with SMN Antibody [2B1] conjugated to Alexa Fluor 488 (NB100-1936AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0012.jpg)
![Flow Cytometry: SMN Antibody (2B1) [NB100-1936] - An intracellular stain was performed on Neuro2a cells with SMN Antibody (2B1) NB100-1936 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Flow-Cytometry-NB100-1936-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - The SMN antibody was tested at a 1:250 dilution in HeLa cells against DyLight 488 (Green). Actin nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0004.jpg)
![Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN on mouse brain using NB100-1936.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunohistochemistry-NB100-1936-img0001.jpg)
![Flow Cytometry: SMN Antibody (2B1) [NB100-1936] - An intracellular stain was performed on Ntera2 cells with SMN Antibody (2B1) NB100-1936 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Flow-Cytometry-NB100-1936-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - CWC22 is colocalized with nuclear speckles & upregulated in diabetic DRG sensory neurons. (A) Subcellular distribution of TDP-43 & CWC22 in control DRG sensory neurons. TDP-43 was stained diffusely in the nucleus, excluding SMN foci in sensory neurons. CWC22 consistently colocalized with a marker protein SC35 of nuclear speckles in sensory neurons. No obvious differences in the subcellular localization of CWC22 were identified in diabetic neurons (not shown) compared with controls. Scale bar: 10 μm. (B) qRT-PCR analysis of Cwc22 mRNA expression in diabetic & control mice. Cwc22 expression was upregulated ∼2.5-fold in diabetic DRGs. *P<0.05, unpaired two-tailed Student's t-test. Data represented as mean±s.e.m. See Cheng et al. (2015) for microarray data indicating rises in Cwc22 expression as reported separately. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/dmm/article/10/3/215/2257/Diabetic-polyneuropathy-sensory-neurons-nuclear), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1936_mouse-monoclonal-smn-antibody-2b1-3102024168396.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Bovine ocular fluid. Antibody at 1:1000. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NBP2-24915-img0006.jpg "Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Bovine ocular fluid. Antibody at 1:1000. Western blot image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Analysis of Superoxide Dismutase 1 in FFPE human liver tissue using an isotype control (top) and NBP2-24915 (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP2-24915-img0003.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Analysis of SOD in liver lysate of 1) human, 2) mouse, and 3) rat using this antibody. 025 ug/ml.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NBP2-24915-img0004.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Human ocular fluid. Antibody at 1:1000. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NBP2-24915-img0005.jpg)
![Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.](http://images.novusbio.com/fullsize/NF-L-Antibody-Western-Blot-NB300-131-img0004.jpg "Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Neurofilament (NF-L antibody) used as axon marker in WT and KO mice using immunofluorescence staining to illustrate the axon morphology of retinal ganglion cells. Central part of retina is enlarged and displayed in WT and KO mice. Scale bars are 500 micrometers for left and 200 micrometers for right. Citation: Lin Y-S, Kuo K-T, Chen S-K, Huang H-S (2018) RBFOX3/NeuN is dispensable for visual function. PLoS ONE 13(2): e0192355. //doi.org/10.1371/journal.pone.0192355](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunofluorescence-NB300-131-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody [NB300-131] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA](http://images.novusbio.com/fullsize/68kDa-Neurofilament-Light-Antibody-Immunocytochemistry-Immunofluorescence-NB300-131-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Analysis of mouse cerebellum section stained with rabbit pAb to NF-L, NB300-131, dilution 1:5,000 in red, and costained with chicken pAb to MBP, dilution 1:5,000, in green. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-131 antibody labels dendrites and axons of neuronal cells, and MBP antibody stains the network of myelin sheathes around axons.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NB300-131-img0003.jpg)
![Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NB300-132 (lane 4).](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Western-Blot-NB300-132-img0003.jpg "Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NB300-132 (lane 4).")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (DA2) [NB300-132] - Staining of neurons in culture using NF-L monoclonal DA2 antibody (green) imaged with a 40X lens. Nuclei are stained blue. Photo Courtesy of Dr. Gerry Shaw.](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Immunocytochemistry-Immunofluorescence-NB300-132-img0004.jpg)
![Simple Western: NF-L Antibody (DA2) [NB300-132] - Image shows a specific band for NF-L in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Simple-Western-NB300-132-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90186-img0022.jpg "Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-90186-img0009.jpg)
![Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Staining of human pancreas shows very weak nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-90186-img0018.jpg)
![Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-90186-img0010.jpg)
![Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-90186-img0011.jpg)
![Simple Western: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Simple Western lane view shows a specific band for SOD1 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Simple-Western-NBP1-90186-img0006.jpg)
![Simple Western: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Electropherogram image(s) of corresponding Simple Western lane view. SOD1/Cu-Zn SOD antibody was used at 1:30 dilution on RT-4 and U-251MG sp lysates(s).](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Simple-Western-NBP1-90186-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SOD1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NBP1-90186-img0023.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NBP2-20534-img0015.jpg "Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - SOD1 antibody detects SOD1 protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOD1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP2-20534-img0014.jpg)
![Western Blot: Superoxide Dismutase 1 Antibody [NBP2-20534] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Superoxide-Dismutase-1-Antibody-Western-Blot-NBP2-20534-img0002.jpg)
![Western Blot: Superoxide Dismutase 1 Antibody [NBP2-20534] - Sample (50 ug of whole cell lysate) A: Rat brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Superoxide-Dismutase-1-Antibody-Western-Blot-NBP2-20534-img0003.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - SOD1 antibody detects SOD1 protein by western blot analysis. Whole cell extracts (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody at a dilution of 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NBP2-20534-img0013.jpg)
![Immunohistochemistry-Frozen: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - Immunofluorescence photomicrographs of paraffin-embedded mouse fetal brain. Green: SOD1 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Red: beta Tubulin 3/ TUJ1 antibody diluted at 1:100. The signal was developed using goat anti-mouse IgG antibody (Dylight594). Blue: Nuclear staining with Hoechst 33342. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunohistochemistry-Frozen-NBP2-20534-img0010.jpg)
![ELISA: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - ELISA detection of SOD1 using SOD1 Antibody for detection at a concentration of 1.5 ug/mL.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-ELISA-NBP2-20534-img0012.jpg)
![Western Blot: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of GluR2 on rat brain lysates with Rabbit anti-GluR2 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 99 kDa Observed band size: 90 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Western-Blot-NBP2-75510-img0005.jpg "Western Blot: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of GluR2 on rat brain lysates with Rabbit anti-GluR2 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 99 kDa Observed band size: 90 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunocytochemistry-Immunofluorescence-NBP2-75510-img0002.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GluR2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunohistochemistry-Paraffin-NBP2-75510-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunocytochemistry-Immunofluorescence-NBP2-75510-img0001.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded mouse brain tissue using anti-GluR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunohistochemistry-Paraffin-NBP2-75510-img0003.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded rat brain tissue using anti-GluR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunohistochemistry-Paraffin-NBP2-75510-img0004.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-GluR2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunohistochemistry-Paraffin-NBP2-75510-img0006.jpg)
![Western Blot: NF-L Antibody (CL4729) [NBP2-59061] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Western-Blot-NBP2-59061-img0008.jpg "Western Blot: NF-L Antibody (CL4729) [NBP2-59061] - Analysis in human cerebral cortex tissue.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (CL4729) [NBP2-59061] - Staining of PC-3 cells using the Anti-NEFL monoclonal antibody, showing specific staining in intermediate filaments in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunocytochemistry-Immunofluorescence-NBP2-59061-img0010.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of human cerebral cortex shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-Paraffin-NBP2-59061-img0009.jpg)
![Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of rat cerebral cortex shows strong immunoreactivity in neuronal cell bodies and processes.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-NBP2-59061-img0001.jpg)
![Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of mouse cerebral cortex shows strong immunoreactivity in neuronal cell bodies and processes.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-NBP2-59061-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - Staining of human cerebellum shows strong cytoplasmic immunoreactivity in cell bodies and proximal dendrites of Purkinje cells.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-Paraffin-NBP2-59061-img0003.jpg)
![Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of rat brainstem shows strong immunoreactivity in neuronal cell bodies.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-NBP2-59061-img0004.jpg)
![Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of mouse striatum shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-NBP2-59061-img0005.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - Staining of human liver shows no positivity as expected (negative control).](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-Paraffin-NBP2-59061-img0007.jpg)
![Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample(30ug whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-31465-img0007.jpg "Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample(30ug whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Ndufs4 Antibody [NBP1-31465] - Analysis of methanol-fixed HeLa, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31465-img0003.jpg)
![Immunohistochemistry-Paraffin: Ndufs4 Antibody [NBP1-31465] - Human ovarian cancer, using NDUFS4 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Immunohistochemistry-Paraffin-NBP1-31465-img0004.jpg)
![Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-31465-img0005.jpg)
![Western Blot: Ndufs4 Antibody [NBP1-31465] - 50 ug Rat heart lysate/extract 12% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-31465-img0006.jpg)
![Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (HL1652) - Azide and BSA Free [NBP3-25693] - SOD1 antibody [HL1652] detects SOD1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded dog brain. SOD1 stained by SOD1 antibody [HL1652] (NBP3-25693) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25693_rabbit-sod1-cu-zn-sod-mab-hl1652-azide-and-bsa-free-212024939181.jpg "Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (HL1652) - Azide and BSA Free [NBP3-25693] - SOD1 antibody [HL1652] detects SOD1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded dog brain. SOD1 stained by SOD1 antibody [HL1652] (NBP3-25693) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Genetic Strategies Validation. </b>Western Blot: SOD1/Cu-Zn SOD Antibody (HL1652) - Azide and BSA Free [NBP3-25693] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1652] (NBP3-25693) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25693_rabbit-sod1-cu-zn-sod-mab-hl1652-azide-and-bsa-free-2120249383178.jpg)
![Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (HL1652) - Azide and BSA Free [NBP3-25693] - SOD1 antibody [HL1652] detects SOD1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SOD1 stained by SOD1 antibody [HL1652] (NBP3-25693) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25693_rabbit-sod1-cu-zn-sod-mab-hl1652-azide-and-bsa-free-2120249415973.jpg)
![Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (HL1652) - Azide and BSA Free [NBP3-25693] - SOD1 antibody [HL1652] detects SOD1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. SOD1 stained by SOD1 antibody [HL1652] (NBP3-25693) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25693_rabbit-sod1-cu-zn-sod-mab-hl1652-azide-and-bsa-free-2120249374978.jpg)
![Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (HL1652) - Azide and BSA Free [NBP3-25693] - SOD1 antibody [HL1652] detects SOD1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat brain. SOD1 stained by SOD1 antibody [HL1652] (NBP3-25693) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25693_rabbit-sod1-cu-zn-sod-mab-hl1652-azide-and-bsa-free-2120249362756.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody (HL1652) - Azide and BSA Free [NBP3-25693] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1652] (NBP3-25693) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25693_rabbit-sod1-cu-zn-sod-mab-hl1652-azide-and-bsa-free-2120249432871.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody (HL1652) - Azide and BSA Free [NBP3-25693] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1652] (NBP3-25693) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25693_rabbit-sod1-cu-zn-sod-mab-hl1652-azide-and-bsa-free-2120249432851.jpg)
![Western Blot: GluR2 Antibody (9R1X2) [NBP3-15370] - Analysis of extracts of various cell lines, using GluR2 Rabbit mAb (NBP3-15370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Western-Blot-NBP3-15370-img0006.jpg "Western Blot: GluR2 Antibody (9R1X2) [NBP3-15370] - Analysis of extracts of various cell lines, using GluR2 Rabbit mAb (NBP3-15370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: GluR2 Antibody (9R1X2) [NBP3-15370] - Immunofluorescence analysis of SH-SY5Y cells using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunocytochemistry-Immunofluorescence-NBP3-15370-img0003.jpg)
![Immunohistochemistry: GluR2 Antibody (9R1X2) [NBP3-15370] - Immunofluorescence analysis of mouse brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunohistochemistry-NBP3-15370-img0005.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (9R1X2) [NBP3-15370] - Rat brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunohistochemistry-Paraffin-NBP3-15370-img0001.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (9R1X2) [NBP3-15370] - Mouse brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunohistochemistry-Paraffin-NBP3-15370-img0002.jpg)
![Immunohistochemistry: GluR2 Antibody (9R1X2) [NBP3-15370] - Immunofluorescence analysis of rat brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunohistochemistry-NBP3-15370-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: GluR2 Antibody (9R1X2) [GluR2] - Confocal imaging of paraffin-embedded Rat brain tissue using GluR2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15370_rabbit-glur2-mab-9r1x2-52202517162341.jpg)
![Immunocytochemistry/ Immunofluorescence: GluR2 Antibody (9R1X2) [GluR2] - Confocal imaging of paraffin-embedded Mouse brain tissue using GluR2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15370_rabbit-glur2-mab-9r1x2-52202517172841.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Staining of NIH3T3 (A), HEK293 (B), HepG2 (C) and MCF7 (D) lysates (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NB100-60944-img0011.jpg "Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Staining of NIH3T3 (A), HEK293 (B), HepG2 (C) and MCF7 (D) lysates (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-60944-img0013.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.02 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NB100-60944-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-60944-img0012.jpg)