| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-55288SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56351SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-178SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74572SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60491SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-81586SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73605 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74571SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58345SS | |||
![Western Blot: DRP1 Antibody [NB110-55288] - Total protein from human HeLa and MCF7 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-DRP1 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/DRP1-Antibody-Western-Blot-NB110-55288-img0012.jpg "Western Blot: DRP1 Antibody [NB110-55288] - Total protein from human HeLa and MCF7 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-DRP1 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: DRP1 Antibody [NB110-55288] - Analysis of FFPE tissue section of mouse kidney using DRP1 antibody #NB110-55288 at 1:300. The primary antibody bound to DRP1 protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This antibody generated a diffused cytoplasmic staining of DRP1 in the epithelial cells of various tubules and in the cells of glomeruli.](http://images.novusbio.com/fullsize/DRP1-Antibody-Immunohistochemistry-Paraffin-NB110-55288-img0013.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DRP1 Antibody [NB110-55288] - Lane 1: DRP1 knockout. Lane 2: DRP1 wildtype MEFs. Stained with NB110-55288 at 1:500.](http://images.novusbio.com/fullsize/DRP1-Antibody-Western-Blot-NB110-55288-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-DRP1 at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55288-img0015.jpg)
![Immunohistochemistry: DRP1 Antibody [NB110-55288] - Staining of renal tubular epithelium and visceral epithelial cells of the glomerulus. Human kidney cortex, 40X magnification.](http://images.novusbio.com/fullsize/DRP1-Antibody-Immunohistochemistry-NB110-55288-img0005.jpg)
![Western Blot: DRP1 Antibody [NB110-55288] - Assessment of skeletal muscle mitochondrial dynamic markers. Representative western blots. Significant main effect of training: #, p < 0.05; ##, p < 0.01. Significant post-hoc training effect: *, p < 0.05; **, p < 0.01. Data are presented as mean +/- SEM. Image collected and cropped by CiteAb from the following publication (null), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DRP1-Antibody-Western-Blot-NB110-55288-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DRP1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55288-img0017.jpg)
![Flow Cytometry: DRP1 Antibody [NB110-55288] - An intracellular stain was performed on HeLa cells with DRP1 Antibody NB110-55288AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/DRP1-Antibody-Flow-Cytometry-NB110-55288-img0018.jpg)
![Western Blot: DRP1 Antibody [NB110-55288] - Rat spinal cord-DRP1 (81kda). Image from verified customer review.](http://images.novusbio.com/fullsize/DRP1-Antibody-Western-Blot-NB110-55288-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-DRP1 at 5.0 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse Dylight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55288-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DRP1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55288-img0016.jpg)
![Flow Cytometry: DRP1 Antibody [NB110-55288] - An intracellular stain was performed on HeLa cells with NB110-55288 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/DRP1-Antibody-Flow-Cytometry-NB110-55288-img0014.jpg)
![Simple Western: DRP1 Antibody [NB110-55288] - Image shows a specific band for DUX4 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/DRP1-Antibody-Simple-Western-NB110-55288-img0008.jpg)
![Simple Western: DRP1 Antibody - BSA Free [NB110-55288] - Image shows a specific band for DRP1 in 0.1 ug/uL mouse hippocampus tissue lysate. Primary antibody dilution: 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/DRP1-Antibody---BSA-Free-Simple-Western-NB110-55288-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - High magnification confocal image of HeLa cells stained with fibrillarin antibody, dilution 1:100 from concentrated tissue culture media (Green), and costained with chicken vimentin pAb (Red), dilution 1:1,000. Nuclear DNA is revealed with the DAPI stain (Blue). The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0008.jpg "Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - High magnification confocal image of HeLa cells stained with fibrillarin antibody, dilution 1:100 from concentrated tissue culture media (Green), and costained with chicken vimentin pAb (Red), dilution 1:1,000. Nuclear DNA is revealed with the DAPI stain (Blue). The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments.")
![Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Analysis of lysates of cell fractions probed with Fibrillarin antibody, dilution 1:500 (Green): [1] protein standard, [2] C6 cytosol, [3] C6 nuclear, [4] HEK293 cytosol, [5] HEK293 nuclear, [6] NIH-3T3 cytosol and [7] NIH-3T3 nuclear fractions. The band at 37kDa corresponds to the fibrillarin protein detected exclusively in the nuclear fractions.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Western-Blot-NB300-269-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci and ectopic nucleoli and produce more rRNA. A) Wild type nuclei stained with anti-Fibrillarin (red) and a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120662), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0012.jpg)
![Immunohistochemistry-Paraffin: Fibrillarin Antibody (38F3) [NB300-269] - IHC staining of Fibrillarin in human ovarian cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunohistochemistry-Paraffin-NB300-269-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Mouse embryonic fibroblast fibrillarin stained with fibrillarin antibody and Alexa-594 secondary antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0013.jpg)
![Flow Cytometry: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin antibody was tested at 1:400 in HEK293 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Flow-Cytometry-NB300-269-img0004.jpg)
![Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Western blot analysis of Fibrillarin expression on yeast protein extracts using NB300-269.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Western-Blot-NB300-269-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin immunofluorescence in fibroblasts. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - IF Confocal analysis of MCF7 cells using Fibrillarin antibody (NB300-269, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci & ectopic nucleoli & produce more rRNA.A) Wild type nuclei stained with anti-Fibrillarin (red) & a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. C) Quantification of nuclear dots marked with both the rDNA probe & anti-Fibrillarin in wild type & mutant nuclei. 45 nuclei were quantified for each genotype. (p-value: 0,00010132, calculated using T-Test). D) qRT-PCR comparing the levels of 18S RNA transcripts in wild type & Dfhth mutant embryos. The experiment was performed three independent times to be significant (p-value: 0,01046, calculated using T-Test). Error bars in the graph show the standard deviation & have being calculated as in Fig. 1E. The experiment was normalized using the bicoid (bcd) mRNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25794008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-269_mouse-monoclonal-fibrillarin-antibody-38f3-310202416205116.jpg)
![Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci & ectopic nucleoli & produce more rRNA.A) Wild type nuclei stained with anti-Fibrillarin (red) & a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. C) Quantification of nuclear dots marked with both the rDNA probe & anti-Fibrillarin in wild type & mutant nuclei. 45 nuclei were quantified for each genotype. (p-value: 0,00010132, calculated using T-Test). D) qRT-PCR comparing the levels of 18S RNA transcripts in wild type & Dfhth mutant embryos. The experiment was performed three independent times to be significant (p-value: 0,01046, calculated using T-Test). Error bars in the graph show the standard deviation & have being calculated as in Fig. 1E. The experiment was normalized using the bicoid (bcd) mRNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25794008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-269_mouse-monoclonal-fibrillarin-antibody-38f3-310202416205175.jpg)
![Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci & ectopic nucleoli & produce more rRNA.A) Wild type nuclei stained with anti-Fibrillarin (red) & a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. C) Quantification of nuclear dots marked with both the rDNA probe & anti-Fibrillarin in wild type & mutant nuclei. 45 nuclei were quantified for each genotype. (p-value: 0,00010132, calculated using T-Test). D) qRT-PCR comparing the levels of 18S RNA transcripts in wild type & Dfhth mutant embryos. The experiment was performed three independent times to be significant (p-value: 0,01046, calculated using T-Test). Error bars in the graph show the standard deviation & have being calculated as in Fig. 1E. The experiment was normalized using the bicoid (bcd) mRNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25794008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-269_mouse-monoclonal-fibrillarin-antibody-38f3-310202416205111.jpg)
![Western Blot: 5-HT3A Antibody [NB100-56351] - The expression of 5-HT3A receptor increased in the E2 + FS group compared to that in the oil + non-FS group. *P < 0.05 versus the oil + non-FS group. E2: estradiol; FS: forced swim. Image collected and cropped by CiteAb from the following publication (//journals.sagepub.com/doi/10.1177/1744806919859723), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Western-Blot-NB100-56351-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: 5-HT3A Antibody [NB100-56351] - The expression of 5-HT3A receptor increased in the E2 + FS group compared to that in the oil + non-FS group. *P < 0.05 versus the oil + non-FS group. E2: estradiol; FS: forced swim. Image collected and cropped by CiteAb from the following publication (//journals.sagepub.com/doi/10.1177/1744806919859723), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-56351] - Analysisof 5-HT3A in human testis using 5-HT3A antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Immunohistochemistry-Paraffin-NB100-56351-img0010.jpg)
![Western Blot: 5-HT3A Antibody [NB100-56351] - Analysis of 5-HT3A in A: human, B: mouse and C: rat brain tissue lysate using 5-HT3A antibody at 3 ug/mL.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Western-Blot-NB100-56351-img0012.jpg)
![Simple Western: 5-HT3A Antibody [NB100-56351] - Image shows a specific band for 5HT3A in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Simple-Western-NB100-56351-img0007.jpg)
![Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.](http://images.novusbio.com/fullsize/NUMB-Antibody-Western-Blot-NB500-178-img0005.jpg "Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.")
![Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Numb-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: NUMB Antibody [NB500-178] - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Numb Antibody (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Numb-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0007.jpg)
![Simple Western: Numb Antibody [NB500-178] - Simple Western lane view shows a specific band for NUMB in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Numb-Antibody-Simple-Western-NB500-178-img0006.jpg)
![Western Blot: SG2NA Antibody (S68) [NB110-74572] - Detection of SG2NA in NIH3T3 cell lysates using NB110-74572.](http://images.novusbio.com/fullsize/SG2NA-Antibody-S68-Western-Blot-NB110-74572-img0005.jpg "Western Blot: SG2NA Antibody (S68) [NB110-74572] - Detection of SG2NA in NIH3T3 cell lysates using NB110-74572.")
![Immunocytochemistry/Immunofluorescence: SG2NA Antibody (S68) [NB110-74572] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SG2NA (S68) [NB110-74572] at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SG2NA-Antibody-S68-Immunocytochemistry-Immunofluorescence-NB110-74572-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SG2NA Antibody (S68) [NB110-74572] - Immunofluorescent staining of NIH3T3 cells using NB110-74572.](http://images.novusbio.com/fullsize/SG2NA-Antibody-S68-Immunocytochemistry-Immunofluorescence-NB110-74572-img0002.jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot using lysates from U20S parental cell line and Dynamin knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Dynamin Monoclonal Antibody (Catalog # NB110-60491) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Dynamin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Knockout-Validated-NB110-60491-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot using lysates from U20S parental cell line and Dynamin knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Dynamin Monoclonal Antibody (Catalog # NB110-60491) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Dynamin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Simple Western: Dynamin Antibody (3G4B6) [NB110-60491] - Simple Western lane view shows a specific band for Dynamin in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Simple-Western-NB110-60491-img0012.jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using Dynamin mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Western-Blot-NB110-60491-img0003.jpg)
![Immunoprecipitation: Dynamin Antibody (3G4B6) [NB110-60491] - Antibody-bead conjugates were prepared by adding 1.0 ug of antibody to 500 ul of PBS with 0,01% triton X-100 in a microcentrifuge tube and 30ul of protein G Sepharose beads. Tubes were rocked O/N at 4 degrees C followed by several washes to remove unbound antibodies. U2OS were collected in HEPES buffer (20 mM HEPES, 100 mM sodium chloride, 1 mM EDTA, 1% Triton X-100, pH 7.4) supplemented with protease inhibitor. Lysates are rocked 30 min at 4 degrees C and spun at 110,000xg for 15 min at 4 degrees C. One ml aliquots at 1 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4 degrees C. Following centrifugation, the unbound fractions were collected, and beads were subsequently washed three times with 1 ml of HEPES lysis buffer and processed for SDS-PAGE and immunoblot. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Immunoprecipitation-NB110-60491-img0014.jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using anti-Dynamin monoclonal antibody against truncated Dynamin recombinant protein (1), SK-N-SH (2) and NIH3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Western-Blot-NB110-60491-img0002.jpg)
![Immunohistochemistry-Paraffin: Dynamin Antibody (3G4B6) [NB110-60491] - Immunohistochemical analysis of paraffin-embedded human Lymph tissue, glioma tissue and cerebellum tissue, showing membrane localization using Dynamin antibody with DAB staining.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Immunohistochemistry-Paraffin-NB110-60491-img0004.jpg)
![Flow Cytometry: Dynamin Antibody (3G4B6) [NB110-60491] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dynamin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Flow-Cytometry-NB110-60491-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Dynamin Antibody (3G4B6) [NB110-60491] - U2OS WT and DNM1 KO cells were labelled with a green or a far red fluorescence dye, respectively. WT and KO cells were mixed and plated to a 1 :1 ratio on coverslips. Cells were stained with and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (WT), red (antibody staining) and far-red (KO) channels was performed. Representative grayscale image of the red channel is shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Antibody dilution used: 1/1000. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Knockout-Validated-NB110-60491-img0015.jpg)
![Western Blot: BAI1 Antibody [NB110-81586] - analysis of BAI1 in mouse brain tissue using anti-BAI1 antibody. KO mice was used as negative controls (right lane). Image from verified customer review.](http://images.novusbio.com/fullsize/BAI1-Antibody-Western-Blot-NB110-81586-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BAI1 Antibody [NB110-81586] - analysis of BAI1 in mouse brain tissue using anti-BAI1 antibody. KO mice was used as negative controls (right lane). Image from verified customer review.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BAI1 Antibody [NB110-81586] - BAI1 antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/BAI1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-81586-img0001.jpg)
![Immunohistochemistry-Paraffin: BAI1 Antibody [NB110-81586] - A. Staining of mouse brain using NB110-81586. B. Blocking of staining using control peptide, NB110-81586PEP.](http://images.novusbio.com/fullsize/BAI1-Antibody-Immunohistochemistry-Paraffin-NB110-81586-img0003.jpg)
![Western Blot: DRP1 Antibody (3B5) [H00010059-M01] - DNM1L monoclonal antibody (M01), clone 3B5 Analysis of DNM1L expression in K-562.](http://images.novusbio.com/fullsize/DRP1-Antibody-3B5-Western-Blot-H00010059-M01-img0008.jpg "Western Blot: DRP1 Antibody (3B5) [H00010059-M01] - DNM1L monoclonal antibody (M01), clone 3B5 Analysis of DNM1L expression in K-562.")
![Immunohistochemistry-Paraffin: DRP1 Antibody (3B5) [H00010059-M01] - Analysis of monoclonal antibody to DNM1L on formalin-fixed paraffin-embedded human leiomyosarcoma tissue. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/DRP1-Antibody-3B5-Immunohistochemistry-Paraffin-H00010059-M01-img0006.jpg)
![Western Blot: DRP1 Antibody (3B5) [H00010059-M01] - Analysis of DNM1L expression in transfected 293T cell line by DNM1L monoclonal antibody (M01), clone 3B5.Lane 1: DNM1L transfected lysate(79 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/DRP1-Antibody-3B5-Western-Blot-H00010059-M01-img0009.jpg)
![ELISA: DRP1 Antibody (3B5) [H00010059-M01] - Detection limit for recombinant GST tagged DNM1L is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DRP1-Antibody-3B5-Sandwich-ELISA-H00010059-M01-img0007.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Western-Blot-NBP2-02577-img0019.jpg "Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)")
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Profilin 1.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Immunocytochemistry-Immunofluorescence-NBP2-02577-img0013.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human pancreas tissue using anti-Profilin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Immunohistochemistry-Paraffin-NBP2-02577-img0015.jpg)
![Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Profilin 1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Flow-Cytometry-NBP2-02577-img0011.jpg)
![Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Western-Blot-NBP2-02577-img0016.jpg)
![Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Profilin 1 monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Western-Blot-NBP2-02577-img0017.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Western-Blot-NBP2-02577-img0018.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human Kidney tissue using anti-Profilin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Immunohistochemistry-Paraffin-NBP2-02577-img0014.jpg)
![Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Hela cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Flow-Cytometry-NBP2-02577-img0009.jpg)
![Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Jurkat cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Flow-Cytometry-NBP2-02577-img0012.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416113530.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416114766.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-31020241613638.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416114752.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-31020241611472.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416114785.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-31020241613635.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416113538.jpg)
![Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Western-Blot-NB110-74571-img0004.jpg "Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.")
![Immunocytochemistry/Immunofluorescence: Striatin Antibody [NB110-74571] - Immunofluorescent staining of NIH3T3 cells ± blocking peptide using NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74571-img0003.jpg)
![SDS-Page: Recombinant Human DRP1 Protein [H00010059-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-DRP1-Protein-SDS-Page-H00010059-P01-img0002.jpg "SDS-Page: Recombinant Human DRP1 Protein [H00010059-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: CDR2 Antibody [NB110-58345] - Cdr2 expressed during mitosis, ubiquitinated and degraded during mitotic exit. Upper left, flow cytometry of G1/S- and G2/M-arrested HEK293s. Upper right, western blots of HEK293 G1/S and G2/M extracts probed with anti-cdr2 (NB110; top), cyclinB1 (middle) and gamma-tubulin (bottom). Bottom panel, cdr2 and cyclin B1 protein quantitation, normalized here and in D to gamma--tubulin levels; *p<0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0010045), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CDR2-Antibody-Western-Blot-NB110-58345-img0009.jpg "Western Blot: CDR2 Antibody [NB110-58345] - Cdr2 expressed during mitosis, ubiquitinated and degraded during mitotic exit. Upper left, flow cytometry of G1/S- and G2/M-arrested HEK293s. Upper right, western blots of HEK293 G1/S and G2/M extracts probed with anti-cdr2 (NB110; top), cyclinB1 (middle) and gamma-tubulin (bottom). Bottom panel, cdr2 and cyclin B1 protein quantitation, normalized here and in D to gamma--tubulin levels; *p<0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0010045), licensed under a CC-BY license.")
![Immunohistochemistry: CDR2 Antibody [NB110-58345] - Purkinje neurons of the cerebellum.](http://images.novusbio.com/fullsize/CDR2-Antibody-Immunohistochemistry-NB110-58345-img0004.jpg)
![Flow Cytometry: CDR2 Antibody [NB110-58345] - Analysis using the DyLight 488 conjugate of NB110-58345. Thymus epithelial cells CDR2. Image from verified customer review.](http://images.novusbio.com/fullsize/CDR2-Antibody-Flow-Cytometry-NB110-58345-img0008.jpg)
![Western Blot: CDR2 Antibody [NB110-58345] - Analysis of CDR2 antibody on HeLa whole cell.](http://images.novusbio.com/fullsize/CDR2-Antibody-Western-Blot-NB110-58345-img0006.jpg)