| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74245 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74592 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96874-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88146-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72607 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-22440-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-115SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25149SS | |||
![Western Blot: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Western-Blot-NBP2-00795-img0002.jpg "Western Blot: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.")
![Immunocytochemistry/Immunofluorescence: SNAP25 Antibody (4G5) [NBP2-00795] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunocytochemistry-Immunofluorescence-NBP2-00795-img0009.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Human liver tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0008.jpg)
![Flow Cytometry: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAP25 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Flow-Cytometry-NBP2-00795-img0001.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0003.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0004.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0005.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0006.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Human colon tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0007.jpg)
![Western Blot: TRAP1 Antibody (1H8) [NBP2-01707] Analysis of extracts (35ug) from 9 different cell lines by using anti-TRAP1 monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Western-Blot-NBP2-01707-img0008.jpg "Western Blot: TRAP1 Antibody (1H8) [NBP2-01707] Analysis of extracts (35ug) from 9 different cell lines by using anti-TRAP1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TRAP1.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Immunocytochemistry-Immunofluorescence-NBP2-01707-img0007.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Human Kidney tissue using anti-TRAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Immunohistochemistry-Paraffin-NBP2-01707-img0006.jpg)
![Flow Cytometry: TRAP1 Antibody (1H8) [NBP2-01707] - Analysis of Jurkat cells, using anti-TRAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Flow-Cytometry-NBP2-01707-img0001.jpg)
![Western Blot: TRAP1 Antibody (1H8) [NBP2-01707] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAP1.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Western-Blot-NBP2-01707-img0002.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TRAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Immunohistochemistry-Paraffin-NBP2-01707-img0003.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TRAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Immunohistochemistry-Paraffin-NBP2-01707-img0004.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Human endometrium tissue using anti-TRAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Immunohistochemistry-Paraffin-NBP2-01707-img0005.jpg)
![Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - Western Blot analysis of Human Cell lysates showing detection of FKBP52/FKBP4 protein using Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody, Clone Hi52C (NB110-96874). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody (NB110-96874) at 1.5 ug/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Hi52C-Western-Blot-NB110-96874-img0009.jpg "Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - Western Blot analysis of Human Cell lysates showing detection of FKBP52/FKBP4 protein using Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody, Clone Hi52C (NB110-96874). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody (NB110-96874) at 1.5 ug/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody, Clone Hi52C (NB110-96874). Tissue: MCF-7 cells (metastatic mammary gland/breast cell line). Species: Human. Primary Antibody: Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody (NB110-96874) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Courtesy of: Tom Ratajczak, Univ. of W. Australia.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Hi52C-Immunocytochemistry-Immunofluorescence-NB110-96874-img0011.jpg)
![Immunohistochemistry: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - Immunohistochemistry analysis using Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody, Clone Hi52C (NB110-96874). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody (NB110-96874) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Hi52C-Immunohistochemistry-NB110-96874-img0010.jpg)
![Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 ug/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-Hi52C-Western-Blot-NB110-96874-img0006.jpg)
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate. HSC70 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16882-img0010.jpg "Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate. HSC70 antibody dilution: 1:500.")
![Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16882-img0001.jpg)
![Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: A431 B: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16882-img0002.jpg)
![Immunohistochemistry-Paraffin: Hsc70 Antibody [NBP2-16882] - Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16882-img0003.jpg)
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HSC70 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16882-img0005.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP1-32118-img0011.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/GRP94-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32118-img0002.jpg)
![Immunohistochemistry: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Mouse lung. HSP90 beta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-NBP1-32118-img0007.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Sample(30 ug of whole cell lysate)A:293TB:A431 7. 5% SDS PAGE, antibody diluted at 1:1500.](http://images.novusbio.com/fullsize/GRP94-Antibody-Western-Blot-NBP1-32118-img0005.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody diluted at 1:1500. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP1-32118-img0009.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Paraffin-embedded BT483 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-32118-img0003.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Mouse skin. HSP90 beta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-32118-img0006.jpg)
![IHC-WM-gp96/HSP90B1/GRP94 Antibody [NBP1-32118] -Hsp90 beta antibody detects Hsp90 beta protein on whole-mount zebrafish embryos by immunohistochemical analysis.Sample: Paraformaldehyde-fixed zebrafish embryos.Hsp90 beta antibody dilution: 1:200.](http://images.novusbio.com/fullsize/antibody/nbp1-32118_rabbit-polyclonal-gp96-hsp90b1-grp94-antibody-immunohistochemistry-whole-mount-3011202314722..jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Sample (30 ug of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE NBP1-32118 diluted at 1:10000](http://images.novusbio.com/fullsize/nbp1-32118_rabbit-polyclonal-gp96-hsp90b1-grp94-antibody-910202420131243.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human cerebral cortex, skeletal muscle, testis and tonsil using Anti-CCT2 antibody NBP1-88146 (A) shows similar protein distribution across tissues to independent antibody NBP1-88147 (B).](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining in human testis and skeletal muscle tissues using anti-CCT2 antibody. Corresponding CCT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0012.jpg)
![Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0011.jpg)
![Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0010.jpg)
![Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human tonsil.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0017.jpg)
![Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TCP1-beta Antibody [NBP1-88146] - Analysis using Anti-CCT2 antibody NBP1-88146 (A) shows similar pattern to independent antibody NBP1-88147 (B).](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Western-Blot-NBP1-88146-img0015.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp1-88146_rabbit-polyclonal-tcp1-beta-antibody-6520251624419.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp1-88146_rabbit-polyclonal-tcp1-beta-antibody-65202516252810.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0015.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0001.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0002.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0003.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0004.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0005.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0006.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human lung tissue within the normal limits using ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0007.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0008.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0009.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0010.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0011.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0012.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0013.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0014.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0016.jpg)
![Western Blot: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP 60) was performed by loading 50ug of the indicated whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a concentration of 1ug/ml overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM secondary antibody at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Western-Blot-NB120-5479-img0016.jpg "Western Blot: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP 60) was performed by loading 50ug of the indicated whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a concentration of 1ug/ml overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM secondary antibody at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP60) (green) in HeLa and NIH3T3 cells.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0013.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (2E1/53) [NB120-5479] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunohistochemistry-Paraffin-NB120-5479-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Anlysis of Hela Cells at a dilution of 1:200](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Heat Shock Protein 60 monoclonal antibody (2E1/53) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Heat Shock Protein 60 monoclonal antibody (2E1/53) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0012.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (2E1/53) [NB120-5479] - Both normal and cancer biopsies of deparaffinized Human kidney tissue tissues.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunohistochemistry-Paraffin-NB120-5479-img0014.jpg)
![Immunoprecipitation: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP60) was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 2ug of a HSP60 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a concentration of 1ug/ml overnight rotating at 4C, washed in TBST, and probed with a goat anti-mouse IgM secondary antibody at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunoprecipitation-NB120-5479-img0006.jpg)
![Western Blot: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of 50ug of the indicated whole cell lysates and 15ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Western-Blot-NBP2-22440-img0015.jpg "Western Blot: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of 50ug of the indicated whole cell lysates and 15ul of PageRuler Prestained Protein Ladder.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 (HSP60) (green) in HeLa and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP60 monoclonal antibody, at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0014.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Normal deparaffinized human Tonsil tissue.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunohistochemistry-Paraffin-NBP2-22440-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using Anti-Heat Shock Protein 60 Monoclonal Antibody (4B9/89) shows staining in A2058 Cells. Heat Shock Protein 60 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 60 at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using Anti-Heat Shock Protein 60 Monoclonal Antibody (4B9/89) shows staining in ATDC5 Cells. Heat Shock Protein 60 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 60 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using Anti-Heat Shock Protein 60 Monoclonal Antibody (4B9/89) shows staining in Hela Cells. Heat Shock Protein 60 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 60 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using a Hsp60 Monoclonal Antibody in human endothelial cells.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 (Hsp60, green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with (left panel) or without (right panel) a Hsp60 monoclonal antibody, at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin, and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0013.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Cancer biopsies of deparaffinized human Breast carcinoma tissue.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunohistochemistry-Paraffin-NBP2-22440-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Normal deparaffinized human Kidney tissue.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunohistochemistry-Paraffin-NBP2-22440-img0004.jpg)
![Immunoprecipitation: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 (HSP60) was performed on HeLa cells. Antigen:antibody complexes were formed by incubating 500ug whole cell lysate with 2ug of HSP60 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent (HRP) at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunoprecipitation-NBP2-22440-img0002.jpg)
![Western Blot: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Analysis using Anti-ST13 antibody NBP2-47427 (A) shows similar pattern to independent antibody NBP2-48865 (B).](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Western-Blot-NBP2-47427-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Analysis using Anti-ST13 antibody NBP2-47427 (A) shows similar pattern to independent antibody NBP2-48865 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47427-img0004.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human kidney.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0009.jpg)
![Immunohistochemistry: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-NBP2-47427-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human colon, kidney, ovary and testis using Anti-ST13 antibody NBP2-47427 (A) shows similar protein distribution across tissues to independent antibody NBP2-48786 (B).](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0005.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human testis.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0007.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human colon.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0008.jpg)
![Immunohistochemistry-Paraffin: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Staining of human ovary.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-Immunohistochemistry-Paraffin-NBP2-47427-img0010.jpg)
![Western Blot: ABCF2 Antibody [NB400-115] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and ABCF2 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human ABCF2 Polyclonal Antibody (Catalog # NB400-115) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for ABCF2 at approximately 71 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Knockdown-Validated-NB400-115-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ABCF2 Antibody [NB400-115] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and ABCF2 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human ABCF2 Polyclonal Antibody (Catalog # NB400-115) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for ABCF2 at approximately 71 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: ABCF2 Antibody [NB400-115] - Analysis of ABCF2 on Lane 1: HeLa whole cell extracts and Lane 2: NIH/3T3 cell lysates using NB400-115.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NB400-115-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ABCF2 Antibody [NB400-115] - ABCF2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunocytochemistry-Immunofluorescence-NB400-115-img0005.jpg)
![Simple Western: ABCF2 Antibody [NB400-115] - Simple Western lane view shows a specific band for ABCF2 in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Simple-Western-NB400-115-img0006.jpg)
![Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Knockout-Validated-NBP2-25149-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Western-Blot-NBP2-25149-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0008.jpg)
![Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Flow-Cytometry-NBP2-25149-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0003.jpg)