| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1972-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44973 | |||
| Azide and BSA Free | NBP2-34621-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77396SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75477SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81803-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70454 | |||
![Western Blot: HSP90 Antibody (AC88) [NB100-1972] - Analysis of HSP90, mAb (AC88): Lane 1: MW Marker; Lane 2: HSP90 (human), (native) ; Lane 3: HSP90 beta, (human) (recombinant); Lane 4: HSP90a (human), (recombinant); Lane 5: HeLa (heat shocked); Lane 6: 3T3 (heat shocked); Lane 7: PC-12 (heat shocked) ; Lane 8: CHO-K1 (heat shocked).](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Western-Blot-NB100-1972-img0001.jpg "Western Blot: HSP90 Antibody (AC88) [NB100-1972] - Analysis of HSP90, mAb (AC88): Lane 1: MW Marker; Lane 2: HSP90 (human), (native) ; Lane 3: HSP90 beta, (human) (recombinant); Lane 4: HSP90a (human), (recombinant); Lane 5: HeLa (heat shocked); Lane 6: 3T3 (heat shocked); Lane 7: PC-12 (heat shocked) ; Lane 8: CHO-K1 (heat shocked).")
![Immunohistochemistry: HSP90 Antibody (AC88) [NB100-1972] - Analysis of human spleen tissue stained with HSP90, mAb (AC88) at 10 ug/ml.](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Immunohistochemistry-NB100-1972-img0003.jpg)
![Flow (Intracellular): HSP90 Antibody (AC88) [NB100-1972] - An intracellular stain was performed on HeLa cells with HSP90 Antibody (AC88) NB100-1972PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Flow-Intracellular-NB100-1972-img0012.jpg)
![Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - Analysis using the DyLight 488 conjugate of NB100-1972. Staining of 106 Jurkat cells using HSP90 mAb (AC88), DyLight 488 Conjugate at a concentration of 50ug/ml.](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Flow-Cytometry-NB100-1972-img0006.jpg)
![Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - Analysis using the Alexa Fluor 405 conjugated HSP90 antibody, NB100-1972AF405 (purple) and fluorescence minus one control (orange). Staining of HSP90 in Primary CD4+ T-cells derived from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Flow-Cytometry-NB100-1972-img0008.jpg)
![Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - Analysis using the PE conjugate of NB100-1972. Staining of 10^6 Jurkat cells using HSP90 mAb (AC88), R-Phycoerythrin Conjugate at a concentration of 10ug/mL](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Flow-Cytometry-NB100-1972-img0007.jpg)
![Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - An intracellular stain was performed on HeLa cells with HSP90 Antibody (AC88) NB100-1972AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Flow-Cytometry-NB100-1972-img0010.jpg)
![Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - An intracellular stain was performed on Jurkat cells with HSP90 Antibody (AC88) NB100-1972AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Flow-Cytometry-NB100-1972-img0011.jpg)
![Western Blot: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of 25 ug of Hela (Lane 1), HepG2 (Lane 2), and mouse spleen cell lysates (Lane 3) and a molecular weight protein ladder.](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-JJ3-Western-Blot-NB300-576-img0010.jpg "Western Blot: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of 25 ug of Hela (Lane 1), HepG2 (Lane 2), and mouse spleen cell lysates (Lane 3) and a molecular weight protein ladder.")
![Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 at a dilution of 1:500 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-JJ3-Immunocytochemistry-Immunofluorescence-NB300-576-img0006.jpg)
![Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Normal biopsies of deparaffinized Human testis tissue.](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-JJ3-Immunohistochemistry-Paraffin-NB300-576-img0003.jpg)
![Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of 3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-JJ3-Flow-Cytometry-NB300-576-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 at a dilution of 1:500 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-JJ3-Immunocytochemistry-Immunofluorescence-NB300-576-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 at a dilution of 1:500 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-JJ3-Immunocytochemistry-Immunofluorescence-NB300-576-img0005.jpg)
![Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-JJ3-Immunohistochemistry-Paraffin-NB300-576-img0001.jpg)
![Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Normal biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-JJ3-Immunohistochemistry-Paraffin-NB300-576-img0002.jpg)
![Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of Jurkat cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-JJ3-Flow-Cytometry-NB300-576-img0007.jpg)
![Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of Hela cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-JJ3-Flow-Cytometry-NB300-576-img0008.jpg)
![Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells and asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated and subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue and afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-10-13) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NB100-1558-img0015.jpg "Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells and asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated and subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue and afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-10-13) licensed under a CC-BY license.")
![Western Blot: p97/VCP Antibody [NB100-1558] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiVCP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NB100-1558-img0012.jpg)
![Western Blot: p97/VCP Antibody [NB100-1558] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1558 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1557. For blotting immunoprecipitated VCP, NB100-1558 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NB100-1558-img0013.jpg)
![Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20 ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells & asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated & subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue & afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22607136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1558_rabbit-polyclonal-p97-vcp-antibody-31020241535618.jpg)
![Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of 25 ug of mouse brain (lane 1), rat brain (lane 2) and human brain (lane 3) lysates.](http://images.novusbio.com/fullsize/VAMP3-Cellubrevin-Antibody-Western-Blot-NB300-510-img0011.jpg "Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of 25 ug of mouse brain (lane 1), rat brain (lane 2) and human brain (lane 3) lysates.")
![Immunocytochemistry/Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP3 (green) showing staining in the cytoplasm of C6 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/VAMP3-Cellubrevin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-510-img0010.jpg)
![Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of recombinant rat VAMP-3.](http://images.novusbio.com/fullsize/VAMP3-Cellubrevin-Antibody-Western-Blot-NB300-510-img0001.jpg)
![Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP-3 in hela cell extract.](http://images.novusbio.com/fullsize/VAMP3-Cellubrevin-Antibody-Western-Blot-NB300-510-img0002.jpg)
![Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of HeLa cell lysates.](http://images.novusbio.com/fullsize/VAMP3-Cellubrevin-Antibody-Western-Blot-NB300-510-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP3 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/VAMP3-Cellubrevin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-510-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP3 (green) showing staining in the cytoplasm of Neuro-2a cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/VAMP3-Cellubrevin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-510-img0009.jpg)
![Western Blot: HSP27 Antibody (G3.1) [NBP2-32972] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Western-Blot-NBP2-32972-img0008.jpg "Western Blot: HSP27 Antibody (G3.1) [NBP2-32972] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (G3.1) [NBP2-32972] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Immunocytochemistry-Immunofluorescence-NBP2-32972-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffin-embedded human Breast Carcinome stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Immunohistochemistry-Paraffin-NBP2-32972-img0004.jpg)
![Flow Cytometry: HSP27 Antibody (G3.1) [NBP2-32972] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Flow-Cytometry-NBP2-32972-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffim-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Immunohistochemistry-Paraffin-NBP2-32972-img0003.jpg)
![Western Blot: HSP60 Antibody [NBP1-77396] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-77396-img0007.jpg "Western Blot: HSP60 Antibody [NBP1-77396] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77396] - Hsp60 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77396-img0004.jpg)
![Immunohistochemistry: HSP60 Antibody [NBP1-77396] - IHC staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunohistochemistry-NBP1-77396-img0006.jpg)
![Flow (Intracellular): HSP60 Antibody [NBP1-77396] - An intracellular stain was performed on HeLa cells with HSP60 Antibody NBP1-77396AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/HSP60-Antibody-Flow-Intracellular-NBP1-77396-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77396] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77396-img0005.jpg)
![Simple Western: HSP60 Antibody [NBP1-77396] - Image shows a specific band for Hsp60 in 0.05 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-Simple-Western-NBP1-77396-img0009.jpg)
![Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP1-75477-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP1-75477] - Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75477-img0006.jpg)
![Immunohistochemistry: Hsp27 Antibody [NBP1-75477] - Staining of HSP27 in mouse heart using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-NBP1-75477-img0007.jpg)
![Western Blot: Hsp27 Antibody [NBP1-75477] - Analysis of HSP27 in human skeletal muscle lysate.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0008.jpg)
![Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of P Glycoprotein on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Western-Blot-NBP2-67667-img0008.jpg "Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of P Glycoprotein on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0007.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded human liver tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0001.jpg)
![Immunohistochemistry-Frozen: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Mouse (B6) liver tissue. Fixed in ice-cold methanol for 10 minutes. Blocking in 10% serum and 1% BSA. Primary antibody at 5 ug/mL, overnight incubation. Secondary antibody at 2 ug/mL for 1.5 hour (Invitrogen A-11012). Mounting with Vector Vectashield with DAPI. Image from a verified customer review.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Frozen-NBP2-67667-img0004.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded mouse kidney tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0003.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0005.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Rabbit Monoclonal MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Human brain microvascular endothelial cells stained for ABCB1, red and ciliary MDR1/ABCB1 green and nuclear DAPI blue. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-67667_rabbit-monoclonal-mdr1-abcb1-antibody-sn06-42-immunocytochemistry-immunofluorescence-22202415364..png)
![Western Blot: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human peripheral blood cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Western-Blot-NBP2-25183-img0007.jpg "Western Blot: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human peripheral blood cells.")
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - HeLa cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-25183-img0005.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - IHC staining of LAMP-1 [H4A3] catalog number NBP2-25183 in mouse liver. Cytoplasmic and membrane staining was observed in tubule cells and glomeruli.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Immunohistochemistry-Paraffin-NBP2-25183-img0014.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on U-87 cells with LAMP-1/CD107a Antibody (H4A3) NBP2-25183PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0017.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human kidney.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Immunohistochemistry-Paraffin-NBP2-25183-img0006.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on A549 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0009.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Surface staining of human peripheral blood cells with anti-CD107a (H4A3) PE.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0004.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on THP-1 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0008.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) azide free, GAM-APC.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0010.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) APC.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0011.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) Alexa Fluor (R) 488.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-NBP2-25183-img0012.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Analysis of PHA-activated PBMC with anti-CD107a (H4A3) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0013.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on A431 cells with LAMP-1/CD107a Antibody [H4A3] NBP2-25183AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0015.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on U-87 cells with LAMP-1/CD107a Antibody (H4A3) NBP2-25183AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0016.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in human placenta and skeletal muscle tissues. Corresponding gp96/HSP90B1/GRP94 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in human placenta and skeletal muscle tissues. Corresponding gp96/HSP90B1/GRP94 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP1-81803-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81803-img0012.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0025.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0021.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0022.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0023.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0024.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Crystallin AB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Western-Blot-NBP1-47708-img0008.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Crystallin AB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Crystallin AB.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunocytochemistry-Immunofluorescence-NBP1-47708-img0011.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of paraffin-embedded Human pancreas tissue using anti-Crystallin AB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunohistochemistry-Paraffin-NBP1-47708-img0013.jpg)
![Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Crystallin AB antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Flow-Cytometry-NBP1-47708-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of HT29 cells using anti-Crystallin AB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunocytochemistry-Immunofluorescence-NBP1-47708-img0010.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of paraffin-embedded Human endometrium tissue using anti-Crystallin AB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunohistochemistry-Paraffin-NBP1-47708-img0012.jpg)
![Immunoprecipitation: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunoprecipitation-NBP1-47708-img0014.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with AlphaB Crystallin/CRYAB Antibody (OTI6A9).](http://images.novusbio.com/fullsize/nbp1-47708_mouse-alphab-crystallin-cryab-mab-oti6a9-17720241044629.jpg)
![Western Blot: p97/VCP Antibody [NB100-1557] - PC12 whole cell lysate, 30 ug total protein in each lane. Antibody at 1:1000. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NB100-1557-img0008.jpg "Western Blot: p97/VCP Antibody [NB100-1557] - PC12 whole cell lysate, 30 ug total protein in each lane. Antibody at 1:1000. Image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: p97/VCP Antibody [NB100-1557] - Sections of human ovarian carcinoma (upper) and mouse squamous cell carcinoma (lower). Antibody: Affinity purified rabbit anti-VCP used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Immunohistochemistry-NB100-1557-img0007.jpg)
![p97/VCP Antibody [NB100-1557] - Detection of human and mouse VCP by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VCP antibody NB100-1557 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-NB100-1557-img0009.jpg)
![p97/VCP Antibody [NB100-1557] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1557 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1558. For blotting immunoprecipitated VCP, NB100-1557 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-NB100-1557-img0010.jpg)