| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-318SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-209SS | |||
| Azide and BSA Free | NBP2-80885 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06274SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22119SS | |||
| Azide and BSA Free | NBP2-80814 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-210SS | |||
| BSA Free | NB500-210-0.1ml | |||
| Azide and BSA Free | NBP2-80614 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-654SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80836 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56112SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97507-0.2mg | |||
| BSA Free | NBP1-97507SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47470SS | |||
| Azide and BSA Free | NBP2-80757 | |||
![Dual RNAscope ISH-IHC: xCT Antibody [NB300-318] - Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for xCT mRNA (ACD RNAScope Probe, catalog #422688; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog # NB300-318) at 0.25ug/mL with 1 hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.](http://images.novusbio.com/fullsize/xCT-Antibody-Dual-RNAscope-ISH-IHC-NB300-318-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: xCT Antibody [NB300-318] - Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for xCT mRNA (ACD RNAScope Probe, catalog #422688; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog # NB300-318) at 0.25ug/mL with 1 hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Simple Western: xCT Antibody [NB300-318] - Simple Western lane view shows a specific band for xCT using NB300-318 at 25 ug/ml in HeLa and HeLa + DEM cell lysates. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/xCT-Antibody-Simple-Western-NB300-318-img0013.jpg)
![Western Blot: xCT Antibody [NB300-318] - Western blot analysis of protein isolated from subcutaneous tumors derived from in vivo growth of the clones relative to WT-derived tumors revealed that xCT levels remained low, phospho-STAT5 (p-STAT5) levels remained high, and phospho-STAT3 (p-STAT3) levels remained unchanged in the absence of SH-4-54. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0161202), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/xCT-Antibody-Western-Blot-NB300-318-img0015.jpg)
![Immunohistochemistry: xCT Antibody [NB300-318] - xCT staining in the absorptive epithelia of small intestinal villi detected using NB300-318.](http://images.novusbio.com/fullsize/xCT-Antibody-Immunohistochemistry-NB300-318-img0006.jpg)
![Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa cells with NB300-318AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/xCT-Antibody-Flow-Cytometry-NB300-318-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: xCT Antibody [NB300-318] - xCT antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/xCT-Antibody-Immunocytochemistry-Immunofluorescence-NB300-318-img0005.jpg)
![Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa cells with xCT Antibody NB300-318AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/xCT-Antibody-Flow-Cytometry-NB300-318-img0010.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: xCT Antibody [NB300-318] - Total protein from Human HeLa cells treated with and without 0.1 mM Diethyl Maleate for 24 hours was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-xCT in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the increase in xCT expression with treatment.](http://images.novusbio.com/fullsize/xCT-Antibody-Western-Blot-NB300-318-img0007.jpg)
![Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa with xCT Antibody NB300-318 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/xCT-Antibody-Flow-Cytometry-NB300-318-img0008.jpg)
![Simple Western: xCT Antibody [NB300-318] - Simple Western lane view shows a specific band for xCT using NB300-318 at 25 ug/mL in HepG2 cell lysates and antibody at 25 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 63 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/xCT-Antibody-Simple-Western-NB300-318-img0014.jpg)
![Western Blot: xCT Antibody - BSA Free [NB300-318] - Subcutaneous injection into nude mice revealed that (A) MDA-MB-231 SH-4-54-resistant clone #2 proliferated at a slower rate than its wild-type (WT) counterpart in vivo. (B) qPCR demonstrated that xCT mRNA levels were lower in tumours isolated from animals injected with clone #2 relative to WT cells (2 animals per treatment group). (C) Western blot analysis of protein isolated from subcutaneous tumours derived from in vivo growth of the clones relative to WT-derived tumours revealed that xCT levels remained low, phospho-STAT5 (p-STAT5) levels remained high, & phospho-STAT3 (p-STAT3) levels remained unchanged in the absence of SH-4-54. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0161202), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-318_rabbit-polyclonal-xct-antibody-310202415392520.jpg)
![Western Blot: xCT Antibody - BSA Free [NB300-318] - A link between KSHV latency & the MC1-R signaling axis in skin-derived cell lines. (a) Western blot analysis of phosphorylated NF-kappa B p65, MC1-R, & TRP-1 in total cell lysates extracted from MeWo (a) or Mel1700 (b) cells either uninfected (control) or acutely infected with KSHV for 0.3 h, 1 h, 3 h, or 6 h. GAPDH was used as loading control. (c) ImageJ quantitation of the p65 band intensities in (a) & (b) relative to GAPDH controls. (d) Mel1700 cells were infected in 6-well plates with increasing volumes (mL/well) of concentrated supernatant containing infectious KSHV, & total RNA from infected cells was subjected to RT-PCR using primer sets for host anti-inflammatory MC1R, POMC, & SLC7A11. (e) Equal aliquots from the same RNA used in (d) were subjected to RT-PCR analysis for select viral latency & cell growth control genes (i.e., GPCR, LANA, & v-FLIP). (f) Western blot analysis of the melanoma cell marker, Melan A, & anti-inflammatory genes MC1-R, TRP1, & SLC7A11 in total cell lysates of uninfected (−) or chronically infected (+) long-term cultures of MeWo-KSHV & Mel1700-KSHV cells. GAPDH was used as an internal control for both the RT-PCR (e) & western blot assays. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24701351), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-318_rabbit-polyclonal-xct-antibody-31020241621818.jpg)
![Western Blot: xCT Antibody - BSA Free [NB300-318] - A link between KSHV latency & the MC1-R signaling axis in skin-derived cell lines. (a) Western blot analysis of phosphorylated NF-kappa B p65, MC1-R, & TRP-1 in total cell lysates extracted from MeWo (a) or Mel1700 (b) cells either uninfected (control) or acutely infected with KSHV for 0.3 h, 1 h, 3 h, or 6 h. GAPDH was used as loading control. (c) ImageJ quantitation of the p65 band intensities in (a) & (b) relative to GAPDH controls. (d) Mel1700 cells were infected in 6-well plates with increasing volumes (mL/well) of concentrated supernatant containing infectious KSHV, & total RNA from infected cells was subjected to RT-PCR using primer sets for host anti-inflammatory MC1R, POMC, & SLC7A11. (e) Equal aliquots from the same RNA used in (d) were subjected to RT-PCR analysis for select viral latency & cell growth control genes (i.e., GPCR, LANA, & v-FLIP). (f) Western blot analysis of the melanoma cell marker, Melan A, & anti-inflammatory genes MC1-R, TRP1, & SLC7A11 in total cell lysates of uninfected (−) or chronically infected (+) long-term cultures of MeWo-KSHV & Mel1700-KSHV cells. GAPDH was used as an internal control for both the RT-PCR (e) & western blot assays. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24701351), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-318_rabbit-polyclonal-xct-antibody-310202416212337.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of NQO1 protein at 6, 12 and 24 hrs following 15 min treatment of BEAS-2B cells with the oil preparation or time-matched soy oil control. Representative blots from one of three separate experiments are shown above. Densitometric evaluations of each target protein blot normalized to its corresponding GAPDH for all three experiments are provided below. Bars represent mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-12-92), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Western-Blot-NB200-209-img0019.jpg "<b>Biological Strategies Validation. </b>Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of NQO1 protein at 6, 12 and 24 hrs following 15 min treatment of BEAS-2B cells with the oil preparation or time-matched soy oil control. Representative blots from one of three separate experiments are shown above. Densitometric evaluations of each target protein blot normalized to its corresponding GAPDH for all three experiments are provided below. Bars represent mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-12-92), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NQO-1 Antibody (A180) [NB200-209] - NQO1 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Immunocytochemistry-Immunofluorescence-NB200-209-img0010.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Immunohistochemistry-Paraffin-NB200-209-img0014.jpg)
![Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Cytometry-NB200-209-img0018.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO1 in A549 lysates using NB 200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Western-Blot-NB200-209-img0007.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Immunohistochemistry showing Anti-NQ01 on formalin fixed paraffin embedded human kidney tissue](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Immunohistochemistry-Paraffin-NB200-209-img0012.jpg)
![Flow (Intracellular): NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U87MG cells with NQO1 (A180) antibody NBP2-24917 (blue) along with a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Intracellular-NB200-209-img0015.jpg)
![Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO-1 in Human A549 Cell Line by Flow Cytometry. Human A549 cell line was stained with Mouse Anti- NQO-1 Monoclonal Antibody (Catalog # NB200-209, filled histogram), or Mouse IgG1 isotype control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Cytometry-NB200-209-img0016.jpg)
![Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Cytometry-NB200-209-img0017.jpg)
![Simple Western: NQO-1 Antibody (A180) [NB200-209] - Simple Western lane view shows a specific band for NQO-1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Simple-Western-NB200-209-img0013.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of HO-1, NQO1 & GCLm proteins at 6, 12 & 24 hrs following 15 min treatment of BEAS-2B cells with the oil preparation or time-matched soy oil control. Representative blots from one of three separate experiments are shown above. Densitometric evaluations of each target protein blot normalized to its corresponding GAPDH for all three experiments are provided below. Bars represent mean ± SEM. Image collected & cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-12-92), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202415175255.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Equol attenuated ER stress by activating Nrf2 in vitro & in vivo.(A) HUVECs were incubated with different concentrations (1, 10 & 100 nM) of equol for 24 h before treatment with t-BHP (50 μM) for another 6 h. The expression of Nrf2 & NQO1 was detected by western blotting. (C) Total tissue lysates from the thoracic & abdominal aorta of apoE-/- mice with or without equol treatment were immunoblotted with anti-Nrf2 & anti-beta -actin antibodies. (E) Cells were transfected with Nrf2 siRNA for 5~6 h; the medium was then replaced with fresh culture medium, followed by incubation for another 24 h. Thereafter, the cells were treated with equol (100 nM) for 24 h & then were incubated with t-BHP (50 μM) for an additional 6 h. The cells were collected & lysed, & western blot analysis was performed. (B)(D)(F) The bar charts show the quantification of the indicated proteins. (G) HUVECs were transfected with Nrf2 siRNA & treated as described in (C), & cell apoptosis was assayed using the Cell Death Detection ELISAplus Kit. Values are presented as means ± SD. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001 versus the control group, RC group or siRNA treated with no drug group; #p < 0.01, ##p < 0.001 versus the t-BHP-treated group & siRNA plus t-BHP treated group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27907038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416212320.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - S-(-)equol primarily up-regulated Nrf2 protein expression activating ARE-dependent response.(A) Whole EA.hy926 cells lysates of cells treated with 250 nM S-(-)equol, 500 nM daidzein, or 50 µM tBHQ for 24 h were subjected to immunoblot analysis with anti-Nrf2, anti-HO-1, anti-NQO1, & anti-beta -tubulin antibodies. (B) Total HUVECs lysates of cell treated with 250 nM S-(-)equol for 24 h were immunoblotted with anti-Nrf2 & anti-beta -tubulin antibodies. (C) EA.hy926 cells were transfected with plasmids containing an HA-tagged Nrf2 open reading frame & the renilla firefly luciferase gene. Medium was changed after 6 hours of incubation. Where indicated, extracts from HA-Nrf2 EA hy.92 cells were additionally probed with anti-HA antibody, anti-Nrf2, anti-HO-1, anti-NQO1 & anti-beta -tubulin antibodies. Values are means of three independent experiments with standard deviations represented by vertical bars. Mean values were significantly different compared to controls (*p<0.05). Total RNA was extracted from EA.hy926 cells or HUVECs treated as indicated, & Nrf2 (D), HO-1 (E), & NQO1 (F) mRNA was measured by real-time RT-PCR analysis. The values shown represent the mean ± SD obtained for three independent experiments (*p<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416205135.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - S-(-)equol primarily up-regulated Nrf2 protein expression activating ARE-dependent response.(A) Whole EA.hy926 cells lysates of cells treated with 250 nM S-(-)equol, 500 nM daidzein, or 50 µM tBHQ for 24 h were subjected to immunoblot analysis with anti-Nrf2, anti-HO-1, anti-NQO1, & anti-beta -tubulin antibodies. (B) Total HUVECs lysates of cell treated with 250 nM S-(-)equol for 24 h were immunoblotted with anti-Nrf2 & anti-beta -tubulin antibodies. (C) EA.hy926 cells were transfected with plasmids containing an HA-tagged Nrf2 open reading frame & the renilla firefly luciferase gene. Medium was changed after 6 hours of incubation. Where indicated, extracts from HA-Nrf2 EA hy.92 cells were additionally probed with anti-HA antibody, anti-Nrf2, anti-HO-1, anti-NQO1 & anti-beta -tubulin antibodies. Values are means of three independent experiments with standard deviations represented by vertical bars. Mean values were significantly different compared to controls (*p<0.05). Total RNA was extracted from EA.hy926 cells or HUVECs treated as indicated, & Nrf2 (D), HO-1 (E), & NQO1 (F) mRNA was measured by real-time RT-PCR analysis. The values shown represent the mean ± SD obtained for three independent experiments (*p<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-31020241620372.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Phf2 protects the liver from high fat & high sucrose diet-induced fibrogenesis during obesity. GFP or Phf2 were overexpressed through AAV strategy specifically in the liver of 7-week-old male C57Bl/6 J mice. Mice were then fed with either a chow diet (NCD) or with a high fat & high sucrose diet (HFHSD) for 16 weeks. Mice were studied in the fed state. a Representative western blot analysis showing the contribution of Phf2 overexpression to the regulation of the PI3K/Akt signaling & pro-fibrogenic pathways in liver extracts (n = 15 per group). b (Left) Oral glucose tolerance test & insulin levels during the OGTT test. (right) Insulin tolerance test (n = 15 per group). c Liver sections stained with hematoxylin & eosin (H&E), trichrome masson & sirius red are shown. Scale bars = 100 μm (n = 10 per group). d Percentage of fibrotic area & percentage of apoptotic hepatocytes (n = 15 per group). e Relative oxidized GSSG content & measurement of Gpx activity (n = 10 per group). f Levels of carbonylated proteins (n = 10 mice per group). All error bars represent mean ± SEM. Statistical analyses were made using unpaired t-test (b) or Anova, followed by Bonferonni’s test (d, e). *P < 0.01 HFHSD/GFP compared to HFHSD/Phf2, $P < 0.01 HFHSD/GFP compared to NCD/GFP, £P < 0.01 HFHSD/Phf2 compared to HFHSD/GFP Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04361-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-31020241621823.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Activation of Nrf2 signaling pathway by FST in RAW 264.7 mouse macrophage-like cells. Cells were treated with FST with or without 100 ng/mL RANKL for 5 days. (A) Total cellular proteins were isolated from cells & the expression levels of Nrf2 & its regulatory proteins were assessed by Western blot analysis. beta -actin was used as the internal control. (C) The expression of nuclear & cytosol Nrf2 were determined by Western blotting. Lamin B & beta -actin were used as internal controls for the nuclear & cytosolic fractions, respectively. The results shown are representative of three independent experiments. (B,D) Statistical analyses were conducted using analysis of variances between groups. * p < 0.05 & *** p < 0.0001 when compared to control. # p < 0.05 & ### p < 0.0001 when compared to RANKL treatment. RANKL: receptor of activator of nuclear factor kappa-B ligand; FST: fermented sea tangle extract; Nrf2: nuclear factor-erythroid 2-related factor 2; p-Nrf2: phosphorylated nuclear factor-erythroid 2-related factor 2; HO-1: heme oxygenase-1; NQO-1: NAD(P)H quinone oxidoreductase 1; +: cells treated the reagent; -: cells untreated the reagent. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31357503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416205186.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Nrf2-pathway activation by hybrids: nuclear translocation & targets induction. (A) Nuclear cellular extracts of SH-SY5Y cells were treated for 3 hours with compounds at 5 µM, 500 nM, & 50 nM or with 20, 10, & 5 µM dimethyl fumarate (DMF). Nrf2 protein content in the nucleus was determined by Western blot. Anti-lamin A/C was used as a protein loading control. Results are shown as ratio Nrf2/lamin A/C ± SEM; *p < 0.05, **p < 0.01 & ****p < 0.0001 versus CTR; Dunnett’s multiple comparison test (F ratio = 6.797, n≥3). (B–C) RNA from total cellular extracts of SH-SY5Y cells, treated for 24 hours with 5 μM compounds or 20 μM DMF, were analyzed for NQO1 (B) & HO-1 (C) mRNA expression by RT-qPCR. GAPDH was used as housekeeping gene. Results are shown as mean ± SEM; *p < 0.05, **p < 0.01, & ***p < 0.001 versus CTR; Dunnett’s multiple comparison test (B, n≥3, F ratio = 10.44; C, n≥3, F ratio = 13.95). (D–E) Cellular extracts of SH-SY5Y cells treated for 24 hours with compounds at 5 μM or 20 μM DMF were analyzed for NQO1 (D) & HO-1 (E) protein levels by Western blot. Anti-actin was used as protein loading control. Results are shown as ratio (% of CTR) ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, & ****p < 0.0001 versus CTR; Dunnett’s multiple comparison test (D, n ≥ 3, F ratio = 5.144; E, n≥3, F ratio = 17.26). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32047434), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416205137.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - The effects of PI3K/Akt & ER inhibitors on S-(-)equol-induced Nrf2 activation in endothelial cells.(A) EA.hy926 cells or HUVECs transfected w/ ARE-dependent firefly luciferase reporter gene (F) & the renilla firefly luciferase gene (R), & treated w/ 250 nM S-(-)equol in the presence or absence of 10 µM LY294002 or 100 nM ICI182,780 for 16 h. The potency of induction is expressed as the relative luminescence (R/F) measured using the dual luciferase reporter assay system (mean ± SD, n = 3). *p<0.05 versus w/ control group, #p<0.05 versus w/ S-(-)equol treated group. (B) EA.hy926 cells or HUVECs incubated w/ or w/out 10 µM LY294002 or 100 nM ICI182,780 for 30 min & then w/ or w/out 250 nM S-(-)equol or 500 nM daidzein for 24 h. Whole cell lysates then immunoblotted w/ antibodies against Nrf2, HO-1, NQO1, & beta -tubulin. Values are means of three independent experiments w/ standard deviations represented by vertical bars. Mean values significantly different compared w/ controls (*p<0.05). (C) EA.hy926 cells or HUVECs cotransfected w/ the HA-Nrf2 & renilla firefly luciferase expression plasmids treated w/ 250 nM S-(-)equol in the presence or absence of 10 µM LY294002 or 100 nM ICI182,780 for 16 h, & HA-Nrf2 localization analyzed by confocal microscopy. (D) EA.hy926 cells incubated w/ 250 nM S-(-)equol, 500 nM daidzein w/ or w/out 10 µM LY294002 or 100 nM ICI182,780 for 16 h & then immunostained w/ an antibody against Nrf2, & Nrf2 localization analyzed by confocal microscopy. Values (intensity of nuclear versus cytoplasmic) are means of counting 100 cells w/ standard deviations represented by vertical bars. *p<0.05 versus w/ control group, #p<0.05 versus w/ S-(-)equol treated group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416205110.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - S-(-)equol primarily up-regulated Nrf2 protein expression activating ARE-dependent response.(A) Whole EA.hy926 cells lysates of cells treated with 250 nM S-(-)equol, 500 nM daidzein, or 50 µM tBHQ for 24 h were subjected to immunoblot analysis with anti-Nrf2, anti-HO-1, anti-NQO1, & anti-beta -tubulin antibodies. (B) Total HUVECs lysates of cell treated with 250 nM S-(-)equol for 24 h were immunoblotted with anti-Nrf2 & anti-beta -tubulin antibodies. (C) EA.hy926 cells were transfected with plasmids containing an HA-tagged Nrf2 open reading frame & the renilla firefly luciferase gene. Medium was changed after 6 hours of incubation. Where indicated, extracts from HA-Nrf2 EA hy.92 cells were additionally probed with anti-HA antibody, anti-Nrf2, anti-HO-1, anti-NQO1 & anti-beta -tubulin antibodies. Values are means of three independent experiments with standard deviations represented by vertical bars. Mean values were significantly different compared to controls (*p<0.05). Total RNA was extracted from EA.hy926 cells or HUVECs treated as indicated, & Nrf2 (D), HO-1 (E), & NQO1 (F) mRNA was measured by real-time RT-PCR analysis. The values shown represent the mean ± SD obtained for three independent experiments (*p<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416212388.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Activation of Nrf2 signaling pathway by FST in RAW 264.7 mouse macrophage-like cells. Cells were treated with FST with or without 100 ng/mL RANKL for 5 days. (A) Total cellular proteins were isolated from cells & the expression levels of Nrf2 & its regulatory proteins were assessed by Western blot analysis. beta -actin was used as the internal control. (C) The expression of nuclear & cytosol Nrf2 were determined by Western blotting. Lamin B & beta -actin were used as internal controls for the nuclear & cytosolic fractions, respectively. The results shown are representative of three independent experiments. (B,D) Statistical analyses were conducted using analysis of variances between groups. * p < 0.05 & *** p < 0.0001 when compared to control. # p < 0.05 & ### p < 0.0001 when compared to RANKL treatment. RANKL: receptor of activator of nuclear factor kappa-B ligand; FST: fermented sea tangle extract; Nrf2: nuclear factor-erythroid 2-related factor 2; p-Nrf2: phosphorylated nuclear factor-erythroid 2-related factor 2; HO-1: heme oxygenase-1; NQO-1: NAD(P)H quinone oxidoreductase 1; +: cells treated the reagent; -: cells untreated the reagent. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31357503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416203710.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06274] - Image shows a specific band for GRP78 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06274-img0006.jpg "Simple Western: GRP78/HSPA5 Antibody [NBP1-06274] - Image shows a specific band for GRP78 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Biological Strategies Validation. </b>Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - C. elegans granulins impair organismal fitness and resistance to ER stress. Total worm lysates from synchronized day 1 adult granulin-expressing animals were immunoblotted with an anti-GRP78/HSPA5 antibody (3 biological replicates). Anti-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1008295), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06274-img0016.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Analysis of MAM-associated protein levels in control and patients' fibroblasts.(A) Equal amounts from one control and the five patients were loaded (50 ug of total cell lysates) and subjected to Western Blot with anti-Grp75, anti-sigma-1R and anti-Mfn2 antibodies. We used anti-GAPDH antibody to ensure equal amounts of protein loaded in each lane. This result is representative of three independent experiments. PLoS One. 2016 Mar 9;11(3):e0150357. doi: 10.1371/journal.pone.0150357](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06274-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - NIH-3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-GRP78/HSPA5 at 2 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06274-img0013.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-GPR78 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06274-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-GRP78/HSPA5 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06274-img0015.jpg)
![Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on NIH3T3 cells with GRP78/HSPA5 Antibody NBP1-06274AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Flow-Cytometry-NBP1-06274-img0014.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Detection of Bip/Grp78 on HeLa whole cell extracts using NBP1-06274.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06274-img0004.jpg)
![Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - Analysis using Alexa Fluor (R) 488 conjugate of NBP1-06274. An intracellular stain was performed on HeLa cells with GPR78/HSPA5 antibody NBP1-06274 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Flow-Cytometry-NBP1-06274-img0007.jpg)
![Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on Jurkat cells with Alexa Fluor 647 conjugate of GPR78/HSPA5 antibody NBP1-06274AF647 (blue) and a matched isotype control NBP2-24893AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Flow-Cytometry-NBP1-06274-img0008.jpg)
![Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on HeLa with NBP1-06274 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Flow-Cytometry-NBP1-06274-img0012.jpg)
![Western Blot: GRP78/HSPA5 Antibody - BSA Free [NBP1-06274] - Hotair promotes the expression of GRP78 & PD-L1, mediates the proliferation, & helps to regulate the T cell-mediated immune responses in Tu212 & Hep-2 cells. Western blot analysis of GRP78 & PD-L1 after mi-NC or has-miR-30a-5p & vehicle or Hotair transfection in Hep-2 cells (a). The relative expression level of GRP78 & PD-L1 in Hep-2 cells (b). (c–e) The cell viability, apoptosis, & colony formation after mi-NC or has-miR-30a-5p & vehicle or Hotair transfection in Hep-2 cells. (f–h) T cell killing assay of target Tu212 & Hep-2 cells cocultured with PBMC, & ELISA detects the concentration of cytokines (g) IFN-gamma & (h) IL-2. The targeted Tu212 & Hep-2 cells after 24 h transfected with mi-NC or has-miR-30a-5p & vehicle or Hotair were cocultured with PBMC, & the cytotoxicity was measured by the LDH release assay. Data are mean ± SD of at least three independent experiments; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35419461), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06274_rabbit-polyclonal-grp78-hspa5-antibody-310202416175290.jpg)
![Western Blot: GRP78/HSPA5 Antibody - BSA Free [NBP1-06274] - Analysis of protein levels involved in ER stress & Ca2+ homeostasis & processing of mRNA XBP1 in control & patients-derived fibroblasts.(A) Equal amounts from controls & patients were loaded (50 μg of total cell lysates) & subjected to Western Blot with anti-Herp, anti-Grp78 & anti-IP3R1 antibodies. We used anti-Hsp60 antibody to ensure equal amounts of protein loaded in each lane. This result is representative of three independent experiments. Protein quantification was performed by laser densitometry. The ratios between proteins/Hsp60 for each cell line were calculated to determine the expression fold-change relative to control. (B) Data represent mean ± standard deviation of three independent experiments. (C) Equal amounts from controls were loaded (50 μg of total cell lysates) & subjected to Western Blot with anti-Herp & anti-Grp78 antibodies. We used anti-GAPDH antibody to ensure equal amounts of protein loaded in each lane. This result is representative of two independent experiments. (D) Equal amounts from control & patients were loaded (50 μg of total cell lysates) & subjected to Western Blot with anti-phospho-PERK antibody. We used anti-GAPDH antibody to ensure equal amounts of protein loaded in each lane. This result is representative of two independent experiments. (E) RT-PCR analysis of the processing of mRNA XBP1 transcription factor. Tm: tunicamycin; u: XBP1 unspliced form; s: XBP1 spliced form. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0150357), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06274_rabbit-polyclonal-grp78-hspa5-antibody-310202416182615.jpg)
![Western Blot: GRP78/HSPA5 Antibody - BSA Free [NBP1-06274] - UPR Components Activated in A beta -Expressing Flies Are Induced Even Further by Glut1 Overexpression(A) Western blot of eIF2 phosphorylation levels in heads of A beta - & A beta Glut1-expressing flies (+RU) & in controls (-RU), showing no significant difference. Bottom: plotted as means ± SEM (n = 3). Top: a representative gel from the same samples.(B) Grp78 mRNA levels in heads of 18-day-old flies expressing A beta or A beta Glut1 in neurons (+RU) & uninduced controls (-RU), measured by qPCR (relative to eIF1A), plotted as means ± SEM. Genotypes: UAS A beta ; elavGS, UAS A beta /UAS Glut1; elavGS.(C) Quantification of GFP fluorescence in fly brains expressing an Xbp1GFP splicing reporter, plotted as means ± SEM (n = 6–13). Genotypes: elavGS/UAS-Xbp1GFP, UAS A beta ; elavGS/UAS-Xbp1GFP, UAS A beta /UAS Glut1; elavGS/UAS-Xbp1GFP.(D) Spliced Xbp1 mRNA levels in heads of 18-day-old flies expressing A beta or A beta Glut1 in neurons (+RU) & uninduced controls (-RU), measured by qPCR (relative to eIF1A), plotted as means ± SEM(E) Western blot of Grp78 in 14-day-old flies of the same genotypes, plotted below as means ± SEM (n = 6–16). The image is a representative gel of the same samples. Genotypes: UAS A beta ; elavGS, UAS A beta /UAS Glut1; elavGS.∗p ≤ 0.05; ∗∗p ≤ 0.01, by ANOVA. See also Figure S2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27524482), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06274_rabbit-polyclonal-grp78-hspa5-antibody-310202416163730.jpg)
![Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody - BSA Free [NBP1-06274] - C. elegans granulins impair organismal fitness & resistance to ER stress.(A) Wild-type (N2) & pgrn-1(-) animals with & without granulin expression were subjected to ER stress with tunicamycin (5 μg / ml). The fraction developing to L4 stage was quantified (n = 50, 3 biological replicates). (B) Wild-type (N2) & pgrn-1(-) animals with & without C. elegans progranulin over-expression (OE) were subjected to ER stress with tunicamycin (5 μg / ml). The fraction developing to L4 stage was quantified (n = 50, 3 biological replicates). (C) Total worm lysates from synchronized day 1 adult granulin-expressing animals were immunoblotted with an anti-HSP-4/BiP antibody (3 biological replicates). Anti-actin was used as a loading control. (D) Wild-type & pgrn-1(-) animals with & without granulin expression were staged as embryos. Animals were scored for development to L4 stage (n = 50, 12 biological replicates). (E) Measurement of body length at day 1 adulthood (n = 12). (F) Measurement of short-term associative learning (three biological replicates). The glutamate receptor mutant nmr-1(ak4) was used as a positive control. Throughout, error bars show mean ± SEM, one or two-way ANOVA with post-hoc Tukey multiple comparisons test. Comparisons are to wild-type unless otherwise indicated (*P<0.05, ***P<0.001, ****P<0.0001, ns = not significant, wt = wild-type). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31398187), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06274_rabbit-polyclonal-grp78-hspa5-antibody-310202416173547.jpg)
![Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5) and MCF-7 (6) cell lysate.](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-Western-Blot-NBP2-22119-img0007.jpg "Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5) and MCF-7 (6) cell lysate.")
![Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded breast cancer tissues using iNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-22119-img0006.jpg)
![Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin (APC).](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-Flow-Cytometry-NBP2-22119-img0011.jpg)
![Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin (PE).](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-Flow-Cytometry-NBP2-22119-img0010.jpg)
![Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mAb against human iNOS (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-Western-Blot-NBP2-22119-img0002.jpg)
![Flow Cytometry: iNOS Antibody (4E5) - BSA Free [NBP2-22119] - An intracellular stain was performed on NIH3T3 cells with iNOS Antibody (4E5) NBP2-22119 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-BSA-Free-Flow-Cytometry-NBP2-22119-img0013.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded liver cancer tissues using iNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-22119-img0009.jpg)
![Flow Cytometry: iNOS Antibody (4E5) - BSA Free [NBP2-22119] - An intracellular stain was performed on Caco-2 cells with iNOS Antibody (4E5) NBP2-22119 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-BSA-Free-Flow-Cytometry-NBP2-22119-img0012.jpg)
![ELISA: iNOS Antibody (4E5) [NBP2-22119] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-ELISA-NBP2-22119-img0001.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody [NB600-1235] - Formalin fixed paraffin embedded human tonsil stained with Caspase-3 Antibody (NB600-1235).](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Immunohistochemistry-Paraffin-NB600-1235-img0003.jpg "Immunohistochemistry-Paraffin: Caspase-3 Antibody [NB600-1235] - Formalin fixed paraffin embedded human tonsil stained with Caspase-3 Antibody (NB600-1235).")
![Immunohistochemistry-Paraffin: Caspase-3 Antibody [NB600-1235] - Human tonsil.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Immunohistochemistry-Paraffin-NB600-1235-img0002.jpg)
![Immunohistochemistry: Caspase-3 Antibody [NB600-1235] - Stained sections of fish intestine and liver of the control group showed negative staining reactions in all the examined parts of the intestinal and hepatic tissue (black and red arrows) against caspase-3 (NB600-1235) and TNF-alpha (NBP2-61611) antibodies. Nearly 10% of hepatic portal cells reacted positively to caspase-3. Scale bars = 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35624801/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb600-1235_rabbit-polyclonal-caspase-3-antibody-imgenex-img-80432-20920231721154.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Mouse fore brain. Iba1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-19019-img0020.jpg "Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Mouse fore brain. Iba1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] - AIF-1/Iba1 Antibody (1 1:1000, Red) expression in dorsal root ganglia. Green fluorescent Nissl was used as counter-staining. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19019-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Mouse cerebellum stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-19019-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Rat brain stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-19019-img0010.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Rat cerebellum stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-19019-img0011.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Rat hind brain. Iba1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-19019-img0016.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Analysis of paraffin-embedded human hepatoma, using at 1:500 dilution.Iba1 antibody detects AIF-1/Iba1 in the cytosol of Kupffer cell in human hepatoma by immunohistochemical analysis.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-19019-img0018.jpg)
![Immunohistochemistry: AIF-1/Iba1 Antibody [NBP2-19019] - LCN2 mainly expressed on neurons. a Representative immunofluorescence images of LCN2 (green) and AIF-1/Iba1 (red) staining in sections of the hippocampus after surgery. Long scale bar, 600 um; short scale bar, 25 um. Image collected and cropped by CiteAb from the following publication (pubmed.ncbi.nlm.nih.gov/35413913) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-19019_rabbit-polyclonal-aif-1-iba1-antibody-2552023153956.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] - LCN2 mainly expressed on neurons. a Representative immunofluorescence images of LCN2 (green), GFAP (red), NeuN (red) & IbaI (red) staining in sections of the hippocampus after surgery. Long scale bar, 600 µm; short scale bar, 25 µm. b Percentage quantification of LCN2 positive cells (n = 3 mice/group). c Cartoon depicting MACS sorting procedures for isolating CD11b+ & CD11b − cells. d Relative expression of cx3cr1 in CD11b + & CD11b − cells after sorting from all samples. d Relative expression level of Lcn2 mRNA in CD11b + & CD11b − cells from hippocampus 6 h after surgery. e Relative expression level of IL-6 mRNA in CD11b + cells. Data are represented as mean ± SEM. **** P Sample: Frozen-sectioned mouse brain.Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody (NBP2-19019) diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: ice-cold MeOH for 5 min](http://images.novusbio.com/fullsize/nbp2-19019_rabbit-polyclonal-aif-1-iba1-antibody-91020242091448.jpg)
![Western Blot: AIF-1/Iba1 Antibody [NBP2-19019] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 antibody (NBP2-19019) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19019_rabbit-polyclonal-aif-1-iba1-antibody-91020242075976.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - AIF-1/Iba1 antibody detects AIF-1/Iba1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.AIF-1/Iba1 stained by AIF-1/Iba1 antibody (NBP2-19019) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19019_rabbit-polyclonal-aif-1-iba1-antibody-910202420115036.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] - AIF-1/Iba1 antibody detects AIF-1/Iba1 protein at cell membrane by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody (NBP2-19019) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19019_rabbit-polyclonal-aif-1-iba1-antibody-91020242011504.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - AIF-1/Iba1 antibody detects AIF-1/Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.AIF-1/Iba1 stained by AIF-1/Iba1 antibody (NBP2-19019) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19019_rabbit-polyclonal-aif-1-iba1-antibody-910202419593534.jpg)
![Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Iba1 protein stained by Iba1 antibody (NBP2-19019) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19019_rabbit-polyclonal-aif-1-iba1-antibody-immunohistochemistry-frozen-2611202518272320.jpg)
![Western Blot: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - ALM induces HIF-1alpha-dependent apoptosis in PC3 cells. PC3 cells were treated with ALM for 24 h and 48 h at 0.1 uM under normoxia and hypoxia, followed by Western blot for caspase-3. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/5/439), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Western-Blot-NB500-210-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - ALM induces HIF-1alpha-dependent apoptosis in PC3 cells. PC3 cells were treated with ALM for 24 h and 48 h at 0.1 uM under normoxia and hypoxia, followed by Western blot for caspase-3. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/5/439), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Simple Western: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Lane view shows a specific band for Caspase 3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Simple-Western-NB500-210-img0005.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Analysis of a FFPE human spleen section using 1:200 dilution of . The staining was developed using HRP conjugated anti-mouse secondary antibody and DAB reagent. This Caspase 3 antibody generated a specific staining in the cytoplasm of various spleenocytes.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Immunohistochemistry-Paraffin-NB500-210-img0007.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder 1:300 dilution of mouse monoclonal Caspase-3 Antibody (CPP32 4-1-18) (NB500-210, Novus Biologicals), for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer(VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Immunohistochemistry-Paraffin-NB500-210-img0006.jpg)
![Immunohistochemistry-Frozen: Caspase-3 Antibody (CPP32 4-1-18) - BSA Free [NB500-210] - Analysis of murine spleen with Caspase-3 (green), CD3 (red) and DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-BSA-Free-Immunohistochemistry-Frozen-NB500-210-img0009.jpg)
![Western Blot: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Detection of Caspase (19 and 35 kDa) from HEK293 cell extract using (NB500-210). Lanes 1 and 2 contain inactive and active Caspase, respectively.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Western-Blot-NB500-210-img0004.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Rat epithelial cells of the tongue base. Antigen retrieval method: Citrate buffer.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Immunohistochemistry-Paraffin-NB500-210-img0001.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-654] - Effects of in vitro hypoxia on the expression of mouse HIF-1 alpha in HIF-1 alpha+/- and HIF-1 alpha+/+ astrocytes. Confluent astrocyte monolayers of both cell types were exposed to a 6 h in vitro hypoxia. HIF-1 alpha mRNA expression was determined by RT-PCR as described in Materials and Methods. Each bar represents the mean +/- SD of relative density/volumes of the bands on film negatives from at least three experiments. Asterisks and number sign indicate significant difference (p < 0.01; one-way ANOVA, followed by multiple comparisons among means). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Western-Blot-NB100-654-img0009.jpg "<b>Biological Strategies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody [NB100-654] - Effects of in vitro hypoxia on the expression of mouse HIF-1 alpha in HIF-1 alpha+/- and HIF-1 alpha+/+ astrocytes. Confluent astrocyte monolayers of both cell types were exposed to a 6 h in vitro hypoxia. HIF-1 alpha mRNA expression was determined by RT-PCR as described in Materials and Methods. Each bar represents the mean +/- SD of relative density/volumes of the bands on film negatives from at least three experiments. Asterisks and number sign indicate significant difference (p < 0.01; one-way ANOVA, followed by multiple comparisons among means). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license.")
![<b>Biological Strategies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody [NB100-654] - Effects of CoCl2 treatment on HIF-1 alpha expression in mouse astrocytes. Western blots using nuclear proteins show that both hypoxia (H) and CoCl2 (Co) up-regulated HIF-1 alpha protein in HIF-1 alpha+/+ and HIF-1 alpha+/- cells. There was no HIF-1 alpha protein detected in control cells. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Western-Blot-NB100-654-img0008.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-654] - IHC analysis of a formalin-fixed paraffin-embedded tissue section of human endometrium carcinoma AN3CA cell line based xenograft using rabbit polyclonal HIF-1 alpha antibody NB100-654 at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the section was further counterstained using hematoxylin. The tested section depicted mainly a diffused cytoplasmic staining but there were some cells which showed nuclear signal also (representing hypoxic cells).](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-654-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-654] - HIF1-alpha analysis using NB100-654. Image submitted from verified customer review.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Western-Blot-NB100-654-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-654] - Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts using NB100-654.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Western-Blot-NB100-654-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody [NB100-654] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Simple-Western-NB100-654-img0005.jpg)
![Gel Super Shift Assays: HIF-1 alpha Antibody [NB100-654] - Mouse HIF-1 alpha+/+ and HIF-1 alpha+/- cells were exposed to hypoxia or 125 uM CoCl2 for 6 h, nuclear extracts were then prepared and EMSA carried out. HIF-1 isolated from hypoxia or CoCl2-treated cells bound to the wild-type probe (lanes #1-6) but not to the mutant probe (lanes #7-12). HIF-1/DNA complex was detected in hypoxia (H)-or CoCl2 (Co)-treated cells (lanes #2, 3, 5, 6) but not in control (C) cells (lanes #1, 4). More complex (darker band) was seen in hypoxia-or CoCl2-treated HIF-1 alpha+/+ cells (lanes #2, 3) than that in hypoxia-or CoCl2-treated HIF-1 alpha+/- cells (lanes #5, 6). Supershift assay showed that the HIF-1/DNA complex was shifted up in the presence of wild-type (wt) oligo probe and 4 ug HIF-1 alpha antiboby (lanes #13 & 14). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Gel-Super-Shift-Assays-NB100-654-img0011.jpg)
![Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-654] - Effects of CoCl2 treatment on HIF-1 alpha expression in mouse astrocytes. (A) The cells were incubated in the presence or absence of 125 μM CoCl2 for 6 hr. HIF-1 alpha mRNA expression was determined by RT-PCR. Each bar represents the mean ± SD of relative density/volumes of the bands on film negatives from at least three experiments. Asterisk & number sign indicate significant difference compared to relevant controls (p < 0.01; one-way ANOVA, followed by multiple comparisons among means). (B) Western blots using nuclear proteins show that both hypoxia (H) & CoCl2 (Co) up-regulated HIF-1 alpha protein in HIF-1 alpha +/+ & HIF-1 alpha +/- cells. There was no HIF-1 alpha protein detected in control cells (C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17474992), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-654_rabbit-polyclonal-hif-1-alpha-antibody-31020241529191.jpg)
![Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-654] - Aberrant HIF1 alpha expression in PDAC cancer associated fibroblastsA. The level of MCT4 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. B. The level of MCT1 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. C. HIF alpha & MCT4 levels were determined by immunoblotting following the knockdown of HIF1 alpha with RNAi or the induction of hypoxia through the use of CoCl2. D. The stability of HIF1 alpha was evaluated in CAFs following treatment with cycloheximide. E. HIF1 alpha was detected by immunoblotting in the presence of cycloheximide in the absence or presence of the proteasome inhibitor MG132. F. HIF1 alpha protein levels were determined in the absence & presence of DMOG. G. RNA levels of the indicated genes as determined by microarray analyses (**p < 0.01 for tumor cell line vs. CAF). H. MG132 was used to interrogate the synthetic rate of HIF1 alpha protein in the indicated cell lines. I. The expression of HIF1 alpha target genes was evaluated in the indicated cell lines by microarray analysis (**p < 0.01 for tumor cell vs. CAF). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27623078), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-654_rabbit-polyclonal-hif-1-alpha-antibody-31020241553714.jpg)
![Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-654] - Aberrant HIF1 alpha expression in PDAC cancer associated fibroblastsA. The level of MCT4 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. B. The level of MCT1 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. C. HIF alpha & MCT4 levels were determined by immunoblotting following the knockdown of HIF1 alpha with RNAi or the induction of hypoxia through the use of CoCl2. D. The stability of HIF1 alpha was evaluated in CAFs following treatment with cycloheximide. E. HIF1 alpha was detected by immunoblotting in the presence of cycloheximide in the absence or presence of the proteasome inhibitor MG132. F. HIF1 alpha protein levels were determined in the absence & presence of DMOG. G. RNA levels of the indicated genes as determined by microarray analyses (**p < 0.01 for tumor cell line vs. CAF). H. MG132 was used to interrogate the synthetic rate of HIF1 alpha protein in the indicated cell lines. I. The expression of HIF1 alpha target genes was evaluated in the indicated cell lines by microarray analysis (**p < 0.01 for tumor cell vs. CAF). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27623078), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-654_rabbit-polyclonal-hif-1-alpha-antibody-31020241682154.jpg)
![Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-654] - Aberrant HIF1 alpha expression in PDAC cancer associated fibroblastsA. The level of MCT4 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. B. The level of MCT1 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. C. HIF alpha & MCT4 levels were determined by immunoblotting following the knockdown of HIF1 alpha with RNAi or the induction of hypoxia through the use of CoCl2. D. The stability of HIF1 alpha was evaluated in CAFs following treatment with cycloheximide. E. HIF1 alpha was detected by immunoblotting in the presence of cycloheximide in the absence or presence of the proteasome inhibitor MG132. F. HIF1 alpha protein levels were determined in the absence & presence of DMOG. G. RNA levels of the indicated genes as determined by microarray analyses (**p < 0.01 for tumor cell line vs. CAF). H. MG132 was used to interrogate the synthetic rate of HIF1 alpha protein in the indicated cell lines. I. The expression of HIF1 alpha target genes was evaluated in the indicated cell lines by microarray analysis (**p < 0.01 for tumor cell vs. CAF). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27623078), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-654_rabbit-polyclonal-hif-1-alpha-antibody-310202415523946.jpg)
![Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-654] - Aberrant HIF1 alpha expression in PDAC cancer associated fibroblastsA. The level of MCT4 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. B. The level of MCT1 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. C. HIF alpha & MCT4 levels were determined by immunoblotting following the knockdown of HIF1 alpha with RNAi or the induction of hypoxia through the use of CoCl2. D. The stability of HIF1 alpha was evaluated in CAFs following treatment with cycloheximide. E. HIF1 alpha was detected by immunoblotting in the presence of cycloheximide in the absence or presence of the proteasome inhibitor MG132. F. HIF1 alpha protein levels were determined in the absence & presence of DMOG. G. RNA levels of the indicated genes as determined by microarray analyses (**p < 0.01 for tumor cell line vs. CAF). H. MG132 was used to interrogate the synthetic rate of HIF1 alpha protein in the indicated cell lines. I. The expression of HIF1 alpha target genes was evaluated in the indicated cell lines by microarray analysis (**p < 0.01 for tumor cell vs. CAF). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27623078), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-654_rabbit-polyclonal-hif-1-alpha-antibody-31020241553515.jpg)
![Immunohistochemistry: LRP2 Antibody (CD7D5) [NB110-96417] - Representative immunofluorescence stainings of control (Tead4m-z+) and Tead4 maternal/zygotic mutant (Tead4m-z-) embryos for Krt8 and Krt18 (16 cell stage embryos) and Lrp2 and Dab2 (32 to 64 cell stage embryos; Lrp2 and Dab2 were not detected in earlier stage embryos). n indicates total number of embryos analyzed. Scale bar: 25 um. Image collected and cropped by CiteAb from the following publication (elifesciences.org/articles/22906), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunocytochemistry-Immunofluorescence-NB110-96417-img0005.jpg "Immunohistochemistry: LRP2 Antibody (CD7D5) [NB110-96417] - Representative immunofluorescence stainings of control (Tead4m-z+) and Tead4 maternal/zygotic mutant (Tead4m-z-) embryos for Krt8 and Krt18 (16 cell stage embryos) and Lrp2 and Dab2 (32 to 64 cell stage embryos; Lrp2 and Dab2 were not detected in earlier stage embryos). n indicates total number of embryos analyzed. Scale bar: 25 um. Image collected and cropped by CiteAb from the following publication (elifesciences.org/articles/22906), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Staining (paraffin-embedded tissue-sections fixed in formalin) of Megalin in the brush border of the proximal tubule.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunohistochemistry-Paraffin-NB110-96417-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - Immunofluorescence staining of Megalin in the brush border of the proximal tubule.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunocytochemistry-Immunofluorescence-NB110-96417-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-LRP2 Antibody (CD7D5) NB110-96417 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunocytochemistry-Immunofluorescence-NB110-96417-img0007.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Analysis of a FFPE tissue section of mouse kidney using 1:200 dilution of LRP2 [CD7D5] antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunohistochemistry-Paraffin-NB110-96417-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were incubated with anti-LRP2 (CD7D5) at 20 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunocytochemistry-Immunofluorescence-NB110-96417-img0004.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Staining on human kidney at 1:100, overnight incubation at 4C. Image from a review by a confirmed customer.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunohistochemistry-Paraffin-NB110-96417-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NB110-96417] - ICM & TE progenitors show loss of responsiveness to Hippo signaling manipulation at the same time as they loose responsiveness to positional changes. (D) Dox-inducible DN Lats2-IRES-mCherry transgenic embryos imaged before Dox treatment (top panel) & the same embryo was imaged following 24 hr of Dox live (middle panel) & fixed/stained for lineage markers (bottom panel). A representative embryo is shown for each stage. Live mCherry is shown as an extended focus image, IF stainings shown as single plane images. mCherry positive ICMs in mosaic transgenic embryos circled with a dotted line. Arrow points to a rare ICM cell in a 64 cell stage-induced embryo with weak Cdx2 expression, which also co-expressed an ICM marker. Scale bar: 25 µm. n indicates number of transgenic embryos analyzed. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/22906), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96417_mouse-monoclonal-lrp2-antibody-cd7d5-31020241621834.jpg)
![Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NB110-96417] - Impairment of autophagy in Atg7-deficient renal proximal tubular cells. (A) Decrease of Atg7 & increase of LC3-I in 2-month-old Atg7flox/flox;KAP-Cre+ mouse kidney. Atg7 & LC3 Western blot (LC3-I/unconjugated LC3 & LC3-II/lipidated LC3) using whole kidney lysate of Atg7flox/flox;KAP-Cre+ mice. As a control, Atg7flox/flox mouse kidney was employed. The intensity of each band of Atg7 & beta -actin was estimated by densitometry. The ratio of Atg7 to beta -actin was calculated. The amount of Atg7-positive signals in the Atg7flox/flox;KAP-Cre+ mice kidney was about 63% lower than that in the Atg7flox/flox mice kidney (n = 3). Note that LC3-I was increased in the Atg7flox/flox;KAP-Cre+ mouse kidney. (B) The massive accumulation of p62 in kidneys of 2-month old Atg7flox/flox;KAP-Cre+ mouse. The cortico-medullary region of each kidney in 2 month-old Atg7flox/flox;KAP-Cre+ mouse & Atg7flox/flox mouse was recognized with anti-p62 antibody (red). As a marker of renal proximal tubular cells, megalin (green) was employed. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue). (C) Quantification of p62 positive area of 2-month-old Atg7flox/flox;KAP-Cre+ (n = 5) & Atg7flox/flox kidney (n = 4, *** p < 0.01). Data in graphs are expressed as the mean ± SEM. Statistical analyses were performed using a student’s t-test. (D) Age-dependent accumulation of p62 (red) in the Atg7flox/flox;KAP-Cre+ mouse kidney in 1-, 2-, 6-, & 9-month-old Atg7flox/flox;KAP-Cre+ (lower) & Atg7flox/flox (upper) mouse kidney. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31881660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96417_mouse-monoclonal-lrp2-antibody-cd7d5-310202416205167.jpg)
![Western Blot: Caspase-3 Antibody - (Pro and Active) [NB100-56112] - Analysis of Caspase-3. Lysates from Jurkat cells (lane 1), normal mammary tissue (lane 2) and surgical specimens from three invasive ductal carcinomas (lanes 3-5) were normalized for total protein content (50 ug/lane) and western blotted with Caspase-3 Antibody - (Pro and Active). The ~32 kDa pro-Caspase-3 protein was detected in all samples. Active/cleaved Caspase-3 was identified in Jurkat and two ductal carcinomas (14-21 kDa large subunit).](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Pro-and-Active-Western-Blot-NB100-56112-img0010.jpg "Western Blot: Caspase-3 Antibody - (Pro and Active) [NB100-56112] - Analysis of Caspase-3. Lysates from Jurkat cells (lane 1), normal mammary tissue (lane 2) and surgical specimens from three invasive ductal carcinomas (lanes 3-5) were normalized for total protein content (50 ug/lane) and western blotted with Caspase-3 Antibody - (Pro and Active). The ~32 kDa pro-Caspase-3 protein was detected in all samples. Active/cleaved Caspase-3 was identified in Jurkat and two ductal carcinomas (14-21 kDa large subunit).")
![Immunohistochemistry-Paraffin: Caspase-3 Antibody - (Pro and Active) [NB100-56112] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder tissue 1:300 dilution of rabbit polyclonal Caspase-3 Antibody - (Pro and Active)(NB100-56112), for 1 hour at room temperature followed by anti-rabbit IgG VisUCyte HRP polymer(VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Pro-and-Active-Immunohistochemistry-Paraffin-NB100-56112-img0011.jpg)
![Simple Western: Caspase-3 Antibody - (Pro and Active) [NB100-56112] - Simple Western lane view shows a specific band for Caspase 3 in 0.2 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Pro-and-Active-Simple-Western-NB100-56112-img0009.jpg)
![Western Blot: Caspase-3 Antibody - (Pro and Active) [NB100-56112] - Effect of acute Cd exposure on BPH1 cell proliferation, Shh signaling activation, and stem cell markers in a time-dependent manner. Cells were incubated with or without 10 uM Cd for the indicated time. Cell lysates were subjected to western blotting using antibodies against p65, Bcl2, BclxL, XIAP, cleaved caspase-3, cleaved PARP, and actin was used as loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-020-0202-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Pro-and-Active-Western-Blot-NB100-56112-img0012.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody - (Pro and Active) [NB100-56112] - Caspase-3 expression in formalin-fixed, paraffin-embedded human reactive lymph node using NB100-56112 (Caspase-3 Antibody - (Pro and Active)) at 1:2000. Staining is seen in the apoptosis-prone germinal center B lymphocytes of follicles.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Pro-and-Active-Immunohistochemistry-Paraffin-NB100-56112-img0008.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody - (Pro and Active) [NB100-56112] - Analysis of Caspase-3 expression in formalin-fixed, paraffin-embedded human breast ductal carcinoma in situ using this antibody at 1:2000. Staining is seen in the the cancerous ducts, but not in the normal lobulus.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Pro-and-Active-Immunohistochemistry-Paraffin-NB100-56112-img0007.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Lane 1: MW marker; Lane 2: HO-1 (rat), (recombinant); Lane 3: HO-1 (human), (recombinant); Lane 4: HO-2 (human), (recombinant); Lane 5: MDBK cell lysate; Lane 6: Dog liver microsome; Lane 7: Mouse liver microsome.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-HO-1-1-Western-Blot-NBP1-97507-img0004.jpg "Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Lane 1: MW marker; Lane 2: HO-1 (rat), (recombinant); Lane 3: HO-1 (human), (recombinant); Lane 4: HO-2 (human), (recombinant); Lane 5: MDBK cell lysate; Lane 6: Dog liver microsome; Lane 7: Mouse liver microsome.")
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of human spleen tissue stained with HO-1, mAb (HO-1-1) at 10ug/ml.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-HO-1-1-Immunohistochemistry-Paraffin-NBP1-97507-img0003.jpg)
![Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of 10^6 Jurkat cells stained using HO-1 monoclonal antibody (HO-1-1), (Prod. No. ADI-OSA-110), at a concentration of 50 ug/mL](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-HO-1-1-Flow-Cytometry-NBP1-97507-img0005.jpg)
![Effect of Sepsis with Isoflurane or Propofol anesthesia on Liver HIF1a, Bcl2, iNOS and HO-1, Protein expression levels. (A) HIF-1 alpha protein was increased in both anesthetic groups by sepsis (Isoflurane +Sepsis, **p = 0.004 and Propofol + Sepsis, *p = 0.04). (B) Bcl-2 protein was increased in Isoflurane +Sepsis group (**p = 0.004) and no significant change in Propofol + Sepsis (*p = 0.104). (C) Inducible nitric oxide synthase [iNOS, (**p = 0.002)] and (D) Heme oxygenase-1 (HO-1, ***p = 0.0006) was increased in Isoflurane +Sepsis group. Whereas, in animals anesthetized with Propofol, three organ-protective proteins, Bcl-2, iNOS, and HO-1 expression was unchanged significantly. Data are shown in mean +/- S.D, n = 12. *denotes a statistically significant (P ⩽ 0.05) difference between animals exposed to sepsis and controls. Statistical differences between results were evaluated using the t-test. “ns](http://images.novusbio.com/fullsize/nbp1-97507_mouse-monoclonal-ho-1-hmox1-hsp32-antibody-ho-1-1-western-blot-13220261533588.jpg)
![Protein expression data (upper) and representative raw data (lower) for animals anesthetized with isoflurane (bcl-2 [a median + IQR], HIF-1 alpha [b mean + 95% confidence interval (CI)], HO-1 [c mean + 95% CI for isoflurane, median + IQR for propofol], iNOS [d mean + 95% CI for isoflurane, median + IQR for propofol) *denotes a statistically significant difference between animals exposed to sepsis and controls Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31307382), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97507_mouse-monoclonal-ho-1-hmox1-hsp32-antibody-ho-1-1-western-blot-13220261812227.jpg)
![Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Western-Blot-NBP1-47470-img0012.jpg "Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of NIH/3T3 (left) and U251 (right) cells using GSK3 beta mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Immunocytochemistry-Immunofluorescence-NBP1-47470-img0006.jpg)
![Immunohistochemistry: GSK-3 beta Antibody (3D10) [NBP1-47470] - Fap1-inhibition with SLV peptide increases phosphorylation of Fap1-substrates Fas and GSK-3 beta in a murine xenograft model. SW620 cells injected into flanks of athymic Nude mice & tumor volume was checked biweekly. Mice treated weekly with oxaliplatin (days 0, 7 and 14) & injected daily with Fap1 blocking SLV peptide, VLS control peptide, or treated with SLV or VLS peptide alone (n=12). Tumors were simultaneously harvested when control tumors were >2,000 mm3. SLV peptide increases Gsk3-phosphorylation with/without oxaliplatin. IF detection of total versus phospho- GSK-3 beta performed with DAPI staining of nuclei (areas without gland formation were selected). Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.25401), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Immunohistochemistry-NBP1-47470-img0014.jpg)
![Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - An intracellular stain was performed on HeLa cells with GSK-3 beta (3D10) antibody NBP1-47470PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Flow-Cytometry-NBP1-47470-img0013.jpg)
![Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis using GSK3 beta mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysates.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Western-Blot-NBP1-47470-img0008.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3 beta mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Immunohistochemistry-Paraffin-NBP1-47470-img0007.jpg)
![Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - Flow cytometric analysis of Hela cells using GSK3 beta mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Flow-Cytometry-NBP1-47470-img0005.jpg)
![Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple Western lane view shows a specific band for GSK-3 Beta in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Simple-Western-NBP1-47470-img0009.jpg)
![Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Simple-Western-NBP1-47470-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470] - Fap1-inhibition with SLV peptide increases Fas & Gsk3 beta phosphorylation in CD133+ cells in a murine xenograft modelSW620 cells were injected in the flanks of athymic Nude mice & tumor volume was determination biweekly. Mice were treated weekly with oxaliplatin (days 0, 7 & 14) & injected daily with Fap1 blocking SLV peptide or VLS control peptide, or treated with SLV or VLS peptide alone (n=12 per cohort). Tumors were simultaneously harvested from cohorts of mice when control tumors were >2,000 mm3. (A) SLV peptide increases Fas phosphorylation in CD133+ xenograft tumors with or without oxaliplatin. Immunofluorescent detection of phospho-Fas or CD133 was performed with DAPI staining of nuclei. (B) SLV peptide increases Gsk3 beta phosphorylation in CD133+ xenograft tumors with or without oxaliplatin. Immunofluorescent detection of phospho-Gsk3 beta or CD133 was performed with DAPI staining of nuclei. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29899829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47470_mouse-monoclonal-gsk-3-beta-antibody-3d10-310202416171462.jpg)
![Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470] - Fap1-inhibition with SLV peptide increases phosphorylation of Fap1-substrates Fas & Gsk3 beta in a murine xenograft modelSW620 cells were injected in the flanks of athymic Nude mice & tumor volume was determination biweekly. Mice were treated weekly with oxaliplatin (days 0, 7 & 14) & injected daily with Fap1 blocking SLV peptide or VLS control peptide, or treated with SLV or VLS peptide alone (n=12 per cohort). Tumors were simultaneously harvested from cohorts of mice when control tumors were >2,000 mm3. (A) SLV peptide increases gland formation in xenograft tumors with or without oxaliplatin. Histology was analyzed by hematoxylin/ eosin staining. Fap1 expression was determined by immunofluorescence. Relative fluorescent intensity (RFI) of Fap1 staining is indicated below relevant panels. (B) SLV peptide increases Fas-phosphorylation in xenograft tumors with or without by oxaliplatin. Immunofluorescent detection of total versus phospho-Fas was performed with DAPI staining of nuclei. Areas without gland formation were selected for this study. (C) SLV peptide increases Gsk3 beta -phosphorylation with or without oxaliplatin. Immunofluorescent detection of total versus phospho- Gsk3 beta was performed with DAPI staining of nuclei. Areas without gland formation were selected for this study. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29899829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47470_mouse-monoclonal-gsk-3-beta-antibody-3d10-3102024161604.jpg)