| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-78039SS | |||
| Azide and BSA Free | NBP2-80921 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22166SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90264-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-146SS | |||
| Azide and BSA Free | NBP2-80977 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 488 | NB110-37253G | |||
![Western Blot: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Analysis of human tonsil (1), human spleen (2), and human lymph node (3) tissue using CD162 antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/Cutaneous-Lymphocyte-Antigen-CLA-Antibody-HECA-452-Western-Blot-NB100-78039-img0006.jpg "Western Blot: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Analysis of human tonsil (1), human spleen (2), and human lymph node (3) tissue using CD162 antibody at a concentration of 2 ug/ml.")
![Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Human tonsil tissue stained with PSGL-1/CD162 Antibody (HECA-452) at 5ug/mL, and Leica Refine DAB kit. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PSGL-1-CD162-Antibody-HECA-452-Immunohistochemistry-Paraffin-NB100-78039-img0014.jpg)
![Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - A cell surface stain was performed on CD3+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and a matched isotype control NBP2-31379. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems]. A co-stain was performed using CD3 antibody NBP2-25186APC.](http://images.novusbio.com/fullsize/Cutaneous-Lymphocyte-Antigen-CLA-Antibody-HECA-452-Flow-Cell-Surface-NB100-78039-img0008.jpg)
![Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast adenocarcinoma using CLA antibody (clone HECA-452) at 5 ug/ml concentration. The antibody generated a membrane-cytoplasmic staining in the cancerous cells of breast adenocarcinoma. Note:- Besides lymphocytes, CLA has been shown to be present on cells of normal endometrium as well as breast cancer tissues (PMID 19625313 and 22970241).](http://images.novusbio.com/fullsize/Cutaneous-Lymphocyte-Antigen-CLA-Antibody-HECA-452-Immunohistochemistry-Paraffin-NB100-78039-img0004.jpg)
![Flow Cytometry: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Human peripheral blood lymphocytes stained with purified HECA-452, followed by anti-mouse IgG FITC](http://images.novusbio.com/fullsize/Cutaneous-Lymphocyte-Antigen-CLA-Antibody-HECA-452-Flow-Cytometry-NB100-78039-img0005.jpg)
![Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - A cell surface stain was performed on CD14+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and a matched isotype control NBP2-31379. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by rat-Dylight 488 conjugated secondary antibody. A co-stain was performed using CD14 antibody NB100-77758AF488.](http://images.novusbio.com/fullsize/Cutaneous-Lymphocyte-Antigen-CLA-Antibody-HECA-452-Flow-Cell-Surface-NB100-78039-img0007.jpg)
![Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP2-22166-img0006.jpg "Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - 293T cells transfected with IDH2-flag expression construct were fixed and stained with anti-IDH2 antibody followed by Alexa Fluor 488 anti-rabbit secondary antibody. DAPI staining shows nuclei. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22166-img0007.jpg)
![Immunohistochemistry: IDH2 Antibody [NBP2-22166] - Staining of IDH2 in mouse intestine.](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunohistochemistry-NBP2-22166-img0003.jpg)
![Western Blot: IDH2 Antibody [NBP2-22166] - Analysis of Caki-1 cell lysate (A), human skeletal muscle tissue (B), mouse skeletal muscle tissue (C), and rat skeletal muscle tissue (D) using IDH2 antibody at a concentration of 2 ug/mL.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP2-22166-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - IDH2 antibody was tested in U2OS cells with DyLight (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22166-img0001.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0011.jpg "Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.")
![Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 2 ug/ml.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Immunohistochemistry-Paraffin-H00055743-M01-img0008.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Western Blot analysis of CHFR expression in A-431 ( Cat # L015V1 ).](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0002.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi ( Cat # H00055743-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody (M01), clone 1H3-A12 (Cat # H00055743-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0009.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M01), clone 1H3-A12.Lane 1: CHFR transfected lysate (Predicted MW: 72.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Immunohistochemistry-Paraffin-H00055743-M01-img0003.jpg)
![Sandwich ELISA: CHFR Antibody (1H3-A12) [H00055743-M01] - Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Sandwich-ELISA-H00055743-M01-img0010.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-32781] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-32781-img0007.jpg "Western Blot: Aconitase 2 Antibody [NBP1-32781] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody [NBP1-32781] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aconitase 2 protein stained by Aconitase 2 antibody [C1C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32781-img0009.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-32781] - Paraffin-embedded mouse duodenum. Aconitase 2 antibody [C1C3] dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32781-img0006.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-32781] - Sample (20 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE, antibody diluted at 1:20000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-32781-img0004.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-32781] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE, antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-32781-img0005.jpg)
![Immunoprecipitation: Aconitase 2 Antibody [NBP1-32781] - HeLa whole cell extracts using 5 ug of Aconitase 2 antibody [C1C3]. Western blot analysis was performed using Aconitase 2 antibody [C1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunoprecipitation-NBP1-32781-img0011.jpg)
![Western Blot: Aspartate Aminotransferase Antibody [NBP1-54778] - Titration: 1.0ug/ml, Positive Control: NCI-H226 cell lysate.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Western-Blot-NBP1-54778-img0006.jpg "Western Blot: Aspartate Aminotransferase Antibody [NBP1-54778] - Titration: 1.0ug/ml, Positive Control: NCI-H226 cell lysate.")
![Western Blot: Aspartate Aminotransferase Antibody [NBP1-54778] - Human Fetal Liver.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Western-Blot-NBP1-54778-img0004.jpg)
![Western Blot: Aspartate Aminotransferase Antibody [NBP1-54778] - Human NCI-H226.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Western-Blot-NBP1-54778-img0005.jpg)
![Western Blot: PRPS2 Antibody [NBP1-31435] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE PRPS2 antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Western-Blot-NBP1-31435-img0005.jpg "Western Blot: PRPS2 Antibody [NBP1-31435] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE PRPS2 antibody, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PRPS2 Antibody [NBP1-31435] - Analysis of HaLa, using NBP1-31435 at 1:200 dilution.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31435-img0003.jpg)
![Immunohistochemistry: PRPS2 Antibody [NBP1-31435] - Representative immunohistochemistry images of PRPS2 protein expression from the whole TMA with low intensity (b1) in normal prostate tissue (K11, scale bar = 0.2 mm), with low intensity (b4) in prostate cancer tissue (A9, scale bar = 0.2 mm), with intermediate intensity (b3) in prostate cancer tissue (G5, scale bar = 0.2 mm), and with high intensity (b2) in prostate cancer tissue (D2, scale bar = 0.2 mm). The percentage of PRPS2 positive cells in D2 is 90%, in G5 is 70%, and in A9 is 15%. Therefore, the percentage score of the case in D7 is 3 and its total protein expression score is 3 x 3 = 9. The percentage score of the case in H7 is 2 and its total protein expression score is 2 x 2 = 4. The percentage score of the case in E4 is 1 and its total protein expression score is 1 x 1 = 1. Image collected and cropped by CiteAb from the following publication (jcancer.org/v11p1027.htm), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunohistochemistry-NBP1-31435-img0007.jpg)
![Immunohistochemistry-Paraffin: PRPS2 Antibody [NBP1-31435] - Paraffin-embedded Hep3B xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunohistochemistry-Paraffin-NBP1-31435-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PRPS2 Antibody [NBP1-31435] - The effects of PRPS2 knockdown confirmed by qRT-PCR (top) and Western blotting (bottom). Image collected and cropped by CiteAb from the following publication (jcancer.org/v11p1027.htm), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Knockdown-Validated-NBP1-31435-img0006.jpg)
![Western Blot: PRPS2 Antibody [NBP1-31435] - Knockdown of PRPS2 decreased prostate cancer cell growth. (A) The effects of PRPS2 knockdown confirmed by qRT-PCR (top) & Western blotting (bottom). (B) In the CCK‑8 assay, cell viability was decreased in si‑PRPS2 compared with NC. (C) In the plate colony formation assay, colony formation was obviously decreased in si-PRPS2 cells transfected. ***P < 0.001. NC: negative controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31435_rabbit-polyclonal-prps2-antibody-310202415345324.jpg)
![Western Blot: PRPS2 Antibody [NBP1-31435] - Knockdown of PRPS2 inhibits xenograft tumor growth in vivo. (A) PRPS2 shRNA effectively blocked its protein expression in PC-3 cells. Cells were stably transfected with scramble (sh-Ctrl) or PRPS2 shRNA (sh-PRPS2). PRPS2 protein levels were normalized to alpha -Tubulin. (B) Gross observation of xenograft tumor size in NOD/SCID mice. (C & D) Silencing of PRPS2 inhibited the tumor growth, including tumor volume (P < 0.001) & weight (P = 0.028, n = 6). (E) H&E‑stained paraffin‑embedded sections obtained from xenografts of PC-3 cells. (F) Top: Immunohistochemical analysis of Ki-67 in the xenografts; Bottom: The apoptosis in tumor tissues was evaluated by TUNEL assay (×200). Graphical illustrated the quantification of Ki-67 & TUNEL positive cells percentage. ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31435_rabbit-polyclonal-prps2-antibody-310202416163792.jpg)
![Western Blot: PRPS2 Antibody [NBP1-31435] - PRPS2 expression in prostate cancer tissues & prostate cancer cell lines. (A) Representative immunohistochemistry images of PRPS2 protein expression from the whole TMA with low intensity (b1) in normal prostate tissue (K11, scale bar = 0.2 mm), with low intensity (b4) in prostate cancer tissue (A9, scale bar = 0.2 mm), with intermediate intensity (b3) in prostate cancer tissue (G5, scale bar = 0.2 mm), & with high intensity (b2) in prostate cancer tissue (D2, scale bar = 0.2 mm). The percentage of PRPS2 positive cells in D2 is 90%, in G5 is 70%, & in A9 is 15%. Therefore, the percentage score of the case in D7 is 3 & its total protein expression score is 3 × 3 = 9. The percentage score of the case in H7 is 2 & its total protein expression score is 2 × 2 = 4. The percentage score of the case in E4 is 1 & its total protein expression score is 1 × 1 = 1. (B) Western blotting & real-time qPCR analysis of PRPS2 protein & mRNA expression in prostate cancer cell lines & normal prostate epithelial cell lines (RWPE-1). (*P < 0.05; **P < 0.01; ***P < 0.001). PRPS2: Phosphoribosyl pyrophosphate synthetase 2; TMA: tissue microarray. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31435_rabbit-polyclonal-prps2-antibody-3102024161890.jpg)
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody [NBP1-90264] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90264-img0008.jpg "Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody [NBP1-90264] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining in human heart muscle and liver tissues using NBP1-90264 antibody. Corresponding ACO2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0018.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0015.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining of human liver shows very weak positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0017.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining of human duodenum shows strong strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0014.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining of human kidney shows strong strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0016.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-90264] - The regulation mechanism of LOX1 on alveolar epithelial cell apoptosis & fibrosis. (a) Cells were transfected with a LOX1 overexpression plasmid & control vector. ROS levels were determined by DCFH-DA assay in MLE-12 cells treated by TGF-beta 1, TGF-beta 1 plus exosome, TGF-beta 1 plus exosome & vector, & TGF-beta 1 plus exosome & LOX1 overexpression plasmid (n = 3). (b) Detection of cellular mtDNA/18sRNA in each of the above cell groups (n = 3). (c) The expression of LOX1, caspase 3, mtDNA damage markers SIRT3 & ACO2, & NLRP3 was measured by western blotting in each of the above cell groups. (d) The expression of the same signal cascades including LOX1, caspase 3, SIRT3, ACO2, & NLRP3 was confirmed in an animal model with pulmonary fibrosis by western blot assay. Data is shown as the means ± SD, n ≥ 3. ∗∗p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31949877), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-90264_rabbit-polyclonal-aconitase-2-antibody-310202416113522.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-90264] - The regulation mechanism of LOX1 on alveolar epithelial cell apoptosis & fibrosis. (a) Cells were transfected with a LOX1 overexpression plasmid & control vector. ROS levels were determined by DCFH-DA assay in MLE-12 cells treated by TGF-beta 1, TGF-beta 1 plus exosome, TGF-beta 1 plus exosome & vector, & TGF-beta 1 plus exosome & LOX1 overexpression plasmid (n = 3). (b) Detection of cellular mtDNA/18sRNA in each of the above cell groups (n = 3). (c) The expression of LOX1, caspase 3, mtDNA damage markers SIRT3 & ACO2, & NLRP3 was measured by western blotting in each of the above cell groups. (d) The expression of the same signal cascades including LOX1, caspase 3, SIRT3, ACO2, & NLRP3 was confirmed in an animal model with pulmonary fibrosis by western blot assay. Data is shown as the means ± SD, n ≥ 3. ∗∗p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31949877), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-90264_rabbit-polyclonal-aconitase-2-antibody-310202416121946.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-90264] - The regulation mechanism of LOX1 on alveolar epithelial cell apoptosis & fibrosis. (a) Cells were transfected with a LOX1 overexpression plasmid & control vector. ROS levels were determined by DCFH-DA assay in MLE-12 cells treated by TGF-beta 1, TGF-beta 1 plus exosome, TGF-beta 1 plus exosome & vector, & TGF-beta 1 plus exosome & LOX1 overexpression plasmid (n = 3). (b) Detection of cellular mtDNA/18sRNA in each of the above cell groups (n = 3). (c) The expression of LOX1, caspase 3, mtDNA damage markers SIRT3 & ACO2, & NLRP3 was measured by western blotting in each of the above cell groups. (d) The expression of the same signal cascades including LOX1, caspase 3, SIRT3, ACO2, & NLRP3 was confirmed in an animal model with pulmonary fibrosis by western blot assay. Data is shown as the means ± SD, n ≥ 3. ∗∗p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31949877), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-90264_rabbit-polyclonal-aconitase-2-antibody-31020241612192.jpg)
![Western Blot: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Detection of thimet oligopeptidase antibody in HeLa whole cell lysate (30 ug) using NB400-146. Lane 1: 0.5 ug/ml; lane 2: 2 ug/ml. 1 minute ECL exposure.](http://images.novusbio.com/fullsize/Thimet-Oligopeptidase-Antibody-4D6-Western-Blot-NB400-146-img0003.jpg "Western Blot: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Detection of thimet oligopeptidase antibody in HeLa whole cell lysate (30 ug) using NB400-146. Lane 1: 0.5 ug/ml; lane 2: 2 ug/ml. 1 minute ECL exposure.")
![Immunohistochemistry: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Analysis of Thimet Oligopeptidase in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Thimet-Oligopeptidase-Antibody-4D6-Immunohistochemistry-NB400-146-img0004.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [N1N3] diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0012.jpg "Western Blot: Aconitase 2 Antibody [NBP2-15245] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [N1N3] diluted at 1:10000.")
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse intestine. Aconitase 2 antibody [N1N3] dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0011.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0003.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE Aconitase 2 antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0004.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0005.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse kidney. Aconitase 2 antibody [N1N3] dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0007.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse urinary bladder. Aconitase 2 antibody [N1N3] ) dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0008.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse ovary. Aconitase 2 antibody [N1N3] dilution: 1:500](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0010.jpg)
![Immunoprecipitation: Aconitase 2 Antibody [NBP2-15245] - Immunoprecipitation of Aconitase 2 protein from HeLa whole cell extracts using 5 ug of Aconitase 2 antibody [N1N3] . Western blot analysis was performed using Aconitase 2 antibody [N1N3] .EasyBlot anti-Rabbit IgG was used as a secondary reagent](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunoprecipitation-NBP2-15245-img0006.jpg)
![Immunohistochemistry: Aconitase 2 Antibody [NBP2-15245] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Aconitase 2 antibody [N1N3] (NBP2-15245) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-15245_rabbit-polyclonal-aconitase-2-antibody-910202420134052.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (30 ug of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE NBP2-15245 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-15245_rabbit-polyclonal-aconitase-2-antibody-9102024205635.jpg)
![Aconitase 2 antibody [N1N3] detects Aconitase 2 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: Aconitase 2 protein stained by Aconitase 2 antibody [N1N3] (NBP2-15245) diluted at 1:1000.Red: MitoTrackerR Red CMXRos, a mitochondria tracker.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15245_rabbit-polyclonal-aconitase-2-antibody-immunocytochemistry-immunofluorescence-2611202518384020.jpg)
![Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Protein Phosphatase 1 beta Antibody detects Protein Phosphatase 1 beta protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Protein Phosphatase 1 beta stained by Protein Phosphatase 1 beta antibody diluted at 1:3000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-32618-img0020.jpg "Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Protein Phosphatase 1 beta Antibody detects Protein Phosphatase 1 beta protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Protein Phosphatase 1 beta stained by Protein Phosphatase 1 beta antibody diluted at 1:3000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1-beta-Antibody-Western-Blot-NBP1-32618-img0008.jpg)
![Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Mouse hind brain dilution: 1:500.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-32618-img0011.jpg)
![Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Proetin Phosphatase 1 beta Antibody detects PPP1CB protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Proetin Phosphatase 1 beta stained by Proetin Phosphatase 1 beta Antibody diluted at 1:3000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-32618-img0019.jpg)
![Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Protein Phosphatase 1 beta antibody detects Protein Phosphatase 1 beta protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Protein Phosphatase 1 beta stained by Protein Phosphatase 1 beta antibody (NBP1-32618) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-32618_rabbit-polyclonal-protein-phosphatase-1-beta-antibody-91020242041274.jpg)
![Western Blot: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Protein Phosphatase 1 beta antibody (NBP1-32618) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32618_rabbit-polyclonal-protein-phosphatase-1-beta-antibody-91020242014632.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1CB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32618_rabbit-polyclonal-protein-phosphatase-1-beta-antibody-1610202419414542.jpg)
![Protein Phosphatase 1 beta antibody detects Protein Phosphatase 1 beta Protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: Protein Phosphatase 1 beta stained by Protein Phosphatase 1 beta antibody (NBP1-32618) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/nbp1-32213_rabbit-polyclonal-p27-kip1-antibody-immunohistochemistry-frozen-141120251855416.jpg)
![Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).](http://images.novusbio.com/fullsize/HSL-Antibody-Western-Blot-NB110-37253-img0002.jpg "Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).")
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody (673) [NBP2-43572] - HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: IDH2 protein stained by IDH2 antibody [GT673] diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/IDH2-Antibody-673-Immunocytochemistry-Immunofluorescence-NBP2-43572-img0009.jpg "Immunocytochemistry/Immunofluorescence: IDH2 Antibody (673) [NBP2-43572] - HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: IDH2 protein stained by IDH2 antibody [GT673] diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug K562 whole cell lysate/extract D. 30 ug THP-1 whole cell lysate/extract E. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE IDH2 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/IDH2-Antibody-673-Western-Blot-NBP2-43572-img0003.jpg)
![Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 50 ug mouse brain lysate/extract 10 % SDS-PAGE IDH2 antibody [673] dilution: 1:1000.](http://images.novusbio.com/fullsize/IDH2-Antibody-673-Western-Blot-NBP2-43572-img0004.jpg)
![Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 50 ug rat brain lysate/extract 12 % SDS-PAGE IDH2 antibody [673] dilution: 1:1000](http://images.novusbio.com/fullsize/IDH2-Antibody-673-Western-Blot-NBP2-43572-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody (673) [NBP2-43572] - Analysis of HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: IDH2 protein stained by IDH2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/IDH2-Antibody-673-Immunocytochemistry-Immunofluorescence-NBP2-43572-img0001.jpg)
![Immunohistochemistry-Paraffin: IDH2 Antibody (673) [NBP2-43572] - Rat duodenum [673] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/IDH2-Antibody-673-Immunohistochemistry-Paraffin-NBP2-43572-img0012.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (NBP2-43572) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43572_mouse-monoclonal-idh2-antibody-673-western-blot-261120251825320.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (NBP2-43572) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43572_mouse-monoclonal-idh2-antibody-673-western-blot-2611202518255020.jpg)
![IDH2 antibody [GT673] detects IDH2 protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. IDH2 antibody [GT673] (NBP2-43572) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43572_mouse-monoclonal-idh2-antibody-673-immunohistochemistry-paraffin-2611202518451323.jpg)