| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14024-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-59724SS | |||
| Azide and BSA Free | NBP2-80643 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30027SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-78644SS | |||
| Azide and BSA Free | NBP2-80781 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2216SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22166SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56709SS | |||
| Azide and BSA Free | NBP2-80613 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81946-0.025ml | |||
![Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024] - Specific positive staining can be observed in a subpopulation of mouse gastric mucosa cells. Green is FNDC5. Image from verified customer review.](http://images.novusbio.com/fullsize/Irisin-FNDC5-Antibody-Immunohistochemistry-Paraffin-NBP2-14024-img0011.jpg "Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024] - Specific positive staining can be observed in a subpopulation of mouse gastric mucosa cells. Green is FNDC5. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024] - Staining of human skeletal muscle shows granular cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Irisin-FNDC5-Antibody-Immunohistochemistry-Paraffin-NBP2-14024-img0007.jpg)
![Western Blot: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of Irisin/FNDC5 expression levels detected by Western blot in Normal Human Keratinocytes (HaCat) and Larynx Epidermoid Carcinoma 2 (HEp-2) (A). The mean value of the densitometric optical analysis of the 3 repeats of Western blot detecting irisin expression levels (B). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35053200/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-2552023151496.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415291921.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Positive immunohistochemical (IHC) reactions (brown color) indicating strong irisin expression in the apical parts of cancer cells & vesicles; magnification x400. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415363743.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415384153.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415392544.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415384123.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415384172.jpg)
![Western Blot: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression levels detected by Western blot in Normal Human Keratinocytes (HaCat) & Larynx Epidermoid Carcinoma 2 (HEp-2) (A). The mean value of the densitometric optical analysis of the 3 repeats of Western blot detecting irisin expression levels (B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35053200), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415171334.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-31020241530429.jpg)
![Western Blot: CD36 Antibody (D-2712) [NB110-59724] - Detection of CD36 in mouse adipose lysate.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-D-2712-Western-Blot-NB110-59724-img0007.jpg "Western Blot: CD36 Antibody (D-2712) [NB110-59724] - Detection of CD36 in mouse adipose lysate.")
![Immunohistochemistry: CD36 Antibody (D-2712) [NB110-59724] - Staining of CD36 in mouse liver.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-D-2712-Immunohistochemistry-NB110-59724-img0006.jpg)
![Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Western-Blot-NBP1-30027-img0013.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.")
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Angiotensinogen at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30027-img0014.jpg)
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody - BSA Free [NBP1-30027] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of Serpin A8 (NBP1-30027) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-30027-img0015.jpg)
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Immunohistochemistry-Paraffin-NBP1-30027-img0012.jpg)
![Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032] - IHC-Fr of normal feline retina with antibodies ABCA4 3F4 (red) and HCAR (green). ABCA4 antibody (1:1000) labels outer segments of photoreceptors and HCAR antibody labels cones. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-Frozen-NBP1-30032-img0006.jpg "Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032] - IHC-Fr of normal feline retina with antibodies ABCA4 3F4 (red) and HCAR (green). ABCA4 antibody (1:1000) labels outer segments of photoreceptors and HCAR antibody labels cones. IHC-Fr image submitted by a verified customer review.")
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - staining of adult mouse retina showing specific immunolabeling of the ABCA4 protein. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0001.jpg)
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in wild-type (ABCA4+/+) retina. PNA labels cone photoreceptors. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0003.jpg)
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in heterozygous (ABCA4+/-) retina. PNA labels cone photoreceptors. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ABCA4 Antibody (3F4) [NBP1-30032] - Characterization of ABCA4 mRNA expression and western blot analyses of ABCA4 protein levels in the canine retina. Western blot analyses of ABCA4 (above), GAPDH (middle), and RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Western-Blot-NBP1-30032-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in affected (ABCA4-/-) retina. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0005.jpg)
![Western Blot: ABCA4 Antibody (3F4) [NBP1-30032] - Characterization of ABCA4 mRNA expression & western blot analyses of ABCA4 protein levels in the canine retina.(A) Relative ABCA4 mRNA expression levels by quantitative RT-PCR in three different regions in three dogs with different genotypes (ABCA4+/+, ABCA4+/-, & ABCA4-/-), normalized to GAPDH expression. (B) Western blot analyses of ABCA4 (above), GAPDH (middle), & RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-310202415371970.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-3102024161606.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-31020241616064.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-31020241616097.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-310202416175238.jpg)
![Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.](http://images.novusbio.com/fullsize/HSD3B1-Antibody-FDO66Q-Immunohistochemistry-NB110-78644-img0002.jpg "Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.")
![Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Analysis of ApoER2 on mouse brain membrane.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Western-Blot-NB100-2216-img0005.jpg "Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Analysis of ApoER2 on mouse brain membrane.")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2216-img0006.jpg)
![Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP2-22166-img0006.jpg "Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - 293T cells transfected with IDH2-flag expression construct were fixed and stained with anti-IDH2 antibody followed by Alexa Fluor 488 anti-rabbit secondary antibody. DAPI staining shows nuclei. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22166-img0007.jpg)
![Immunohistochemistry: IDH2 Antibody [NBP2-22166] - Staining of IDH2 in mouse intestine.](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunohistochemistry-NBP2-22166-img0003.jpg)
![Western Blot: IDH2 Antibody [NBP2-22166] - Analysis of Caki-1 cell lysate (A), human skeletal muscle tissue (B), mouse skeletal muscle tissue (C), and rat skeletal muscle tissue (D) using IDH2 antibody at a concentration of 2 ug/mL.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP2-22166-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - IDH2 antibody was tested in U2OS cells with DyLight (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22166-img0001.jpg)
![Western Blot: Caspase-3 Antibody (31A893) [NB100-56709] - Curcumin Analogs Induce Apoptosis in Cancerous Cells by Several Pathways. E6-1 cells were treated for 24 hours with or without the broad spectrum caspase inhibitor ZVAD(oMe)-FMK and the studied compounds, lysed and subjected to Western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-01230-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Western-Blot-NB100-56709-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: Caspase-3 Antibody (31A893) [NB100-56709] - Curcumin Analogs Induce Apoptosis in Cancerous Cells by Several Pathways. E6-1 cells were treated for 24 hours with or without the broad spectrum caspase inhibitor ZVAD(oMe)-FMK and the studied compounds, lysed and subjected to Western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-01230-4) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A893) [NB100-56709] - Tissue section of normal human breast using 5 ug/mL concentration of . Very strong diffused as well as granular immunopositivity of Caspase 3 was observed specifically in the cytoplasmic of ductal /acinar epithelial cells.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Immunohistochemistry-Paraffin-NB100-56709-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody (31A893) [NB100-56709] - Left panel: Untreated Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with at 10 ug/mL overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Immunocytochemistry-Immunofluorescence-NB100-56709-img0008.jpg)
![Western Blot: Caspase-3 Antibody (31A893) [NB100-56709] - Analysis for human Caspase-3 using HL60 lysates with Caspase-3 Antibody (31A893) at 2 ug/mL dilution. NB100-56709 only detects a 32 kD Caspase-3 corresponding to pro-Caspase-3.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Western-Blot-NB100-56709-img0005.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Caspase-3 Antibody (31A893) [NB100-56709] - Analysis of Caspase-3 in Jurkat cells. Cells were treated with 2 uM staurosporine for different time periods. Caspase-3 activation is detected in Western blots by the presence of Caspase-3 cleavage fragments. These antibodies detect both pro (full-length) and active (cleaved) Caspase-3, depending on the treatment time points. A basal level of endogenous active Caspase-3 may be see in untreated Jurkat cells.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Western-Blot-NB100-56709-img0006.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A893) [NB100-56709] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder tissue using 5 ug/mL of mouse monoclonal Caspase-3 Antibody (31A893) (NB100-56709, Novus Biologicals), for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Immunohistochemistry-Paraffin-NB100-56709-img0007.jpg)
![Western Blot: Caspase-3 Antibody (31A893) - BSA Free [NB100-56709] - Lemongrass extract is dependent on the production of oxidative stress to induce apoptosis. (E) E6-1 cells treated for 3 hrs with LG with or without NAC, lysed, & subjected to SDS-PAGE. Cells then transferred to a PVDF membrane & probed for the specific proteins. Bands visualized with a chemiluminescence reagent. Statistical calculations performed using Two-Way ANOVA multiple comparison for (A) & One-Way ANOVA multiple comparison for (B–C). **p < 0.01 vs. Control, ****p < 0.0001 vs. Control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56709_mouse-monoclonal-caspase-3-antibody-31a893-31020241535657.jpg)
![Western Blot: Caspase-3 Antibody (31A893) - BSA Free [NB100-56709] - Functioning FADD protein is required to induce apoptosis in cancer cells treated with lemongrass extract(A) U-937 & (B) DN FADD Jurkat cells were treated for 12 hours & 48 hours, respectively, with the specified treatments, lysed, & subjected to SDS-PAGE. Cells were then transferred to a PVDF membrane & probed for the specific proteins. Bands were visualized with a chemiluminescence reagent. (C) DN FADD Jurkat cells were treated for 48 hours with the specified doses & stained with Annexin V & PI. Results were obtained using image-based cytometry with the Y-axis representative of percent of cells positive for Annexin V (green), PI (red), Annexin V & PI (yellow), or negative for both Annexin V & PI (blue). Values are expressed as a mean ± SD from three independent experiments. (D) DN FADD Jurkat cells were treated with H2DCFDA following treatments with paraquat (PQ), LG, or WT for 3 hours. Results were obtained using the image-based cytometry with the Y-axis representative of percent of cells positive for DCF. Values are expressed as a mean ± SD from three independent experiments. Statistical calculations were performed using Two-Way ANOVA multiple comparison for (C) & One-Way ANOVA multiple comparison for (D). ***p < 0.001 vs. Control, ****p < 0.0001 vs. Control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56709_mouse-monoclonal-caspase-3-antibody-31a893-310202415525230.jpg)
![Western Blot: Caspase-3 Antibody (31A893) - BSA Free [NB100-56709] - Functioning FADD protein is required to induce apoptosis in cancer cells treated with lemongrass extract(A) U-937 & (B) DN FADD Jurkat cells were treated for 12 hours & 48 hours, respectively, with the specified treatments, lysed, & subjected to SDS-PAGE. Cells were then transferred to a PVDF membrane & probed for the specific proteins. Bands were visualized with a chemiluminescence reagent. (C) DN FADD Jurkat cells were treated for 48 hours with the specified doses & stained with Annexin V & PI. Results were obtained using image-based cytometry with the Y-axis representative of percent of cells positive for Annexin V (green), PI (red), Annexin V & PI (yellow), or negative for both Annexin V & PI (blue). Values are expressed as a mean ± SD from three independent experiments. (D) DN FADD Jurkat cells were treated with H2DCFDA following treatments with paraquat (PQ), LG, or WT for 3 hours. Results were obtained using the image-based cytometry with the Y-axis representative of percent of cells positive for DCF. Values are expressed as a mean ± SD from three independent experiments. Statistical calculations were performed using Two-Way ANOVA multiple comparison for (C) & One-Way ANOVA multiple comparison for (D). ***p < 0.001 vs. Control, ****p < 0.0001 vs. Control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56709_mouse-monoclonal-caspase-3-antibody-31a893-310202415535124.jpg)
![Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody (1A6) [H00057016-M01] - AKR1B10 monoclonal antibody (M01), clone 1A6 Analysis of AKR1B10 expression in HepG2.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1B10-AKR1B10-Antibody-1A6-Western-Blot-H00057016-M01-img0011.jpg "Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody (1A6) [H00057016-M01] - AKR1B10 monoclonal antibody (M01), clone 1A6 Analysis of AKR1B10 expression in HepG2.")
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1B10/AKR1B10 Antibody (1A6) [H00057016-M01] - Analysis of monoclonal antibody to AKR1B10 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1B10-AKR1B10-Antibody-1A6-Immunocytochemistry-Immunofluorescence-H00057016-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1B10/AKR1B10 Antibody (1A6) [H00057016-M01] - Analysis of monoclonal antibody to AKR1B10 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1B10-AKR1B10-Antibody-1A6-Immunohistochemistry-Paraffin-H00057016-M01-img0009.jpg)
![Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody (1A6) [H00057016-M01] - Analysis of AKR1B10 expression in transfected 293T cell line by AKR1B10 monoclonal antibody (M01), clone 1A6.Lane 1: AKR1B10 transfected lysate(36 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1B10-AKR1B10-Antibody-1A6-Western-Blot-H00057016-M01-img0012.jpg)
![ELISA: Aldo-keto Reductase 1B10/AKR1B10 Antibody (1A6) [H00057016-M01] - Detection limit for recombinant GST tagged AKR1B10 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1B10-AKR1B10-Antibody-1A6-ELISA-H00057016-M01-img0004.jpg)
![Sandwich ELISA: Aldo-keto Reductase 1B10/AKR1B10 Antibody (1A6) [H00057016-M01] - Detection limit for recombinant GST tagged AKR1B10 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1B10-AKR1B10-Antibody-1A6-Sandwich-ELISA-H00057016-M01-img0010.jpg)
![Flow Cytometry: PGC1 alpha Antibody [NB100-60955] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-16520239183346.jpg "Flow Cytometry: PGC1 alpha Antibody [NB100-60955] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-60955] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-1652023918336.jpg)
![Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NB100-60955] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-16520239183311.jpg)
![Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NB100-60955] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-1652023918331.jpg)
![Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-60955] - (6ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-16520239183335.jpg)
![Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Proprotein-Convertase-9-PCSK9-Antibody-Western-Blot-NB300-959-img0005.jpg "Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/NB300-959_Immunohistochemistry-Paraffin-Proprotein-Convertase-9-PCSK9-Antibody-NB300-959-5ug-ml-staining-of-paraffin-embedded-Human-Liver-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--29320239254023.jpg)
![Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.](http://images.novusbio.com/fullsize/NB300-959_Immunohistochemistry-Paraffin-Proprotein-Convertase-9-PCSK9-Antibody-NB300-959-Negative-Control-showing-staining-of-paraffin-embedded-Human-Liver-with-no-primary-antibody--2932023925404.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523259.jpg "Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.")
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523253.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523256.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523250.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, NB100-81946 was diluted at 1:200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-2342024155514.jpg)
![Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Western blot analysis of GSK-3 beta [p Tyr279, p Tyr216] expression in mouse testis (A), rat testis (B), rat spleen (C) whole cell lysates.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-234202415232519.jpg)
![Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Analysis of extracts of various cell lines, using NB100-81946](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-103202523472617.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81946(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-113202594524.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Methanol-fixed MEF cells.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-113202515145457.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Staining of Methanol-fixed Hela cells.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-113202515145471.jpg)