| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05054SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78162SS | |||
| Azide and BSA Free | NBP2-81010 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06398SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87102-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96877-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14024-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30027SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-78644SS | |||
| Azide and BSA Free | NBP2-80781 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22166SS | |||
![Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody and b antigen-preabsorbed antibody. Scale bar 100 um. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) and DAPI (blue) on ependymal cells. Scale bar 10 um. e-g Immunofluorescence staining of GLUT9 (magenta) and NeuN (green) showing co-localization in neurons. Scale bar 10 um. Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-NBP1-05054-img0006.jpg "Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody and b antigen-preabsorbed antibody. Scale bar 100 um. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) and DAPI (blue) on ependymal cells. Scale bar 10 um. e-g Immunofluorescence staining of GLUT9 (magenta) and NeuN (green) showing co-localization in neurons. Scale bar 10 um. Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.")
![Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - GLUT9 in methacarn-fixed murine brain. Paraffin sections of methacarn-fixed murine brain were used for immunostaining. a, b Antigen absorption test. Immunohistochemistry using showing a anti-GLUT9 antibody staining in the third ventricle ependyma and parenchyma and b antigen-preabsorbed antibody. Scale bar 100 um. c GLUT9 immunoreactivity observed in brain capillaries in the cortex. Scale bar 100 um. d-f Immunofluorescence staining of d GLUT9 and e P-gp showing co-localization in capillaries. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 um.Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-NBP1-05054-img0007.jpg)
![Western Blot: GLUT9 Antibody [NBP1-05054] - Detection of GLUT9 in human kidney membrane.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Western-Blot-NBP1-05054-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GLUT9 Antibody [NBP1-05054] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-GLUT9 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05054-img0005.jpg)
![Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-05054] - IHC-P analysis of mouse kidney section using GLUT9 antibody at 1:200 dilution. This antibody generated an expected cytoplasmic staining in tubular cells with a more staining towards membranes in some cells but no immunopositivity in Bowmans capsules.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-Paraffin-NBP1-05054-img0004.jpg)
![Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody & b antigen-preabsorbed antibody. Scale bar 100 µm. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) & DAPI (blue) on ependymal cells. Scale bar 10 µm. e–g Immunofluorescence staining of GLUT9 (magenta) & NeuN (green) showing co-localization in neurons. Scale bar 10 µm. D3V, dorsal third ventricle; DAPI, 4′,6-diamidino-2-phenylindole; NeuN, neuronal nucleus marker Image collected & cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05054_rabbit-polyclonal-glut9-antibody-310202416171452.jpg)
![Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] - Immunofluorescence staining of ABCG2 in methanol/acetone-fixed & methacarn-fixed murine brain. Fresh frozen sections of the dorsal third ventricle were prepared from wild-type (WT) & ABCG2 knockout (KO) mice & post-fixed with methanol & acetone. Immunofluorescence staining of ABCG2 was seen in choroid plexus & capillaries in sections from a WT but not in b ABCG2 KO mouse. Scale bar 100 µm. c Immunofluorescence staining of ABCG2 (magenta) & GLUT9 (green) showing ABCG2 in the choroid plexus & GLUT9 in the ependyma. Scale bar 100 µm. d–f Immunofluorescence staining of d ABCG2 & e GLUT9 using methacarn-fixed paraffin section of capillaries showing co-localization in f. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 µm. CP, choroid plexus; D3V, dorsal third ventricle Image collected & cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05054_rabbit-polyclonal-glut9-antibody-310202416173530.jpg)
![Western Blot: VLDLR Antibody (6A6) [NBP1-78162] - MCF-7 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. VLDLR Antibody (NBP1-78162) antibody at 1:1000, 4C, overnight. WB image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/VLDLR-Antibody-6A6-Western-Blot-NBP1-78162-img0010.jpg "Western Blot: VLDLR Antibody (6A6) [NBP1-78162] - MCF-7 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. VLDLR Antibody (NBP1-78162) antibody at 1:1000, 4C, overnight. WB image submitted by a verifed customer review.")
![Immunocytochemistry/Immunofluorescence: VLDLR Antibody (6A6) [NBP1-78162] - VLDL R Antibody (6A6) [NBP1-78162] - VLDL Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/VLDL-R-Antibody-6A6-Immunocytochemistry-Immunofluorescence-NBP1-78162-img0006.jpg)
![Immunohistochemistry: VLDL R Antibody (6A6) [NBP1-78162] - Analysis of VLDL Receptor in mouse kidney.](http://images.novusbio.com/fullsize/VLDL-R-Antibody-6A6-Immunohistochemistry-NBP1-78162-img0007.jpg)
![Western Blot: VLDL R Antibody (6A6) [NBP1-78162] - Analysis of VLDL Receptor expression in mouse kidney.](http://images.novusbio.com/fullsize/VLDL-R-Antibody-6A6-Western-Blot-NBP1-78162-img0008.jpg)
![Immunohistochemistry: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - GPX3 expressions in adenocarcinoma (AC). EnVision immunohistochemistry, original magnification x200. GPX3 positive reaction was mainly localized in the cytoplasm. Negative GPX3 expression in poorly differentiated AC (c), and positive GPX3 expression in well-differentiated AC (d). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/dm/2013/187043/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Immunohistochemistry-NBP1-06398-img0007.jpg "Immunohistochemistry: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - GPX3 expressions in adenocarcinoma (AC). EnVision immunohistochemistry, original magnification x200. GPX3 positive reaction was mainly localized in the cytoplasm. Negative GPX3 expression in poorly differentiated AC (c), and positive GPX3 expression in well-differentiated AC (d). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/dm/2013/187043/), licensed under a CC-BY license.")
![Immunohistochemistry: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - GPX3 expressions in squamous carcinomas/adenosquamous carcinomas (SC/ASC). EnVision immunohistochemistry, original magnification x200. GPX3 positive reaction was mainly localized in the cytoplasm. Negative GPX3 expression in poorly differentiated SC/ASC (c). Positive GPX3 expression in well-differentiated SC/ASC (d). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/dm/2013/187043/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Immunohistochemistry-NBP1-06398-img0006.jpg)
![Western Blot: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - Analysis of GPX3 in human kidney lysate.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Western-Blot-NBP1-06398-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using 1:200 conc. of Glutathione Peroxidase 3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Glutathione Peroxidase 3 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Immunohistochemistry-Paraffin-NBP1-06398-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - The GPX3 antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI blue).](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06398-img0003.jpg)
![Immunohistochemistry: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - ALDH1A3 & GPX3 expressions in squamous carcinomas/adenosquamous carcinomas (SC/ASC). EnVision immunohistochemistry, original magnification ×200. ALDH1A3 & GPX3 positive reaction was mainly localized in the cytoplasm. (a), positive ALDH1A3 expression in poorly differentiated SC/ASC. (b), negative ALDH1A3 expression in well-differentiated SC/ASC. (c), negative GPX3 expression in poorly differentiated SC/ASC. (d), positive GPX3 expression in well-differentiated SC/ASC. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24167362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06398_rabbit-polyclonal-glutathione-peroxidase-3-gpx3-antibody-31020241517522.jpg)
![Immunohistochemistry: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - ALDH1A3 & GPX3 expressions in adenocarcinoma (AC). EnVision immunohistochemistry, original magnification ×200. ALDH1A3 & GPX3 positive reaction was mainly localized in the cytoplasm. (a), positive ALDH1A3 expression in moderately differentiated AC. (b), negative ALDH1A3 expression in well-differentiated AC. (c), negative GPX3 expression in poorly differentiated AC. (d), positive GPX3 expression in well-differentiated AC. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24167362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06398_rabbit-polyclonal-glutathione-peroxidase-3-gpx3-antibody-310202415291925.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Analysis in human cerebral cortex and liver tissues. Corresponding S100B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Analysis in human cerebral cortex and liver tissues. Corresponding S100B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: S100B Antibody [NBP1-87102] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-Western-Blot-NBP1-87102-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody [NBP1-87102] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining Is shown in green.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87102-img0018.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0025.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in purkinje cell layer.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0022.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0023.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0024.jpg)
![Western Blot: GCLC Antibody (3H1) [H00002729-M01] - Analysis of GCLC expression in transfected 293T cell line by GCLC monoclonal antibody (M01), clone 3H1.Lane 1: GCLC transfected lysate(72.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GCLC-Antibody-3H1-Western-Blot-H00002729-M01-img0013.jpg "Western Blot: GCLC Antibody (3H1) [H00002729-M01] - Analysis of GCLC expression in transfected 293T cell line by GCLC monoclonal antibody (M01), clone 3H1.Lane 1: GCLC transfected lysate(72.8 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: GCLC Antibody (3H1) [H00002729-M01] - Analysis of monoclonal antibody to GCLC on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/GCLC-Antibody-3H1-Immunocytochemistry-Immunofluorescence-H00002729-M01-img0008.jpg)
![Western Blot: GCLC Antibody (3H1) [H00002729-M01] - GCLC monoclonal antibody (M01), clone 3H1. Analysis of GCLC expression in PC-12.](http://images.novusbio.com/fullsize/GCLC-Antibody-3H1-Western-Blot-H00002729-M01-img0010.jpg)
![Western Blot: GCLC Antibody (3H1) [H00002729-M01] - GCLC monoclonal antibody (M01), clone 3H1 Analysis of GCLC expression in A-431.](http://images.novusbio.com/fullsize/GCLC-Antibody-3H1-Western-Blot-H00002729-M01-img0011.jpg)
![Western Blot: GCLC Antibody (3H1) [H00002729-M01] - GCLC monoclonal antibody (M01), clone 3H1. Analysis of GCLC expression in NIH/3T3.](http://images.novusbio.com/fullsize/GCLC-Antibody-3H1-Western-Blot-H00002729-M01-img0012.jpg)
![ELISA: GCLC Antibody (3H1) [H00002729-M01] - Detection limit for recombinant GST tagged GCLC is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GCLC-Antibody-3H1-ELISA-H00002729-M01-img0009.jpg)
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) diluted in 1.5% BSA and TBST for 1 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG: HRP at 1:4000 for 1 hour at RT with shaking . Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0018.jpg "Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) diluted in 1.5% BSA and TBST for 1 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG: HRP at 1:4000 for 1 hour at RT with shaking . Predicted/Observed Size: Multiple Bands.")
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0021.jpg)
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human A549 cells showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (NB110-96877) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0016.jpg)
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human Recombinant Protein showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane 2: hASYN Monomer (3.84 ug). Lane 3: Nitrosylated hASYN (3.84 ug).. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 2 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0017.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0014.jpg)
![Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immuno-peroxidase (DAB) staining for nitrotyrosine (upper row) and immuno-alkaline phosphatase (ALP) staining for 8-OHdG (bottom row), hematoxylin counterstain. Representative small intestinal tumor histopathology of Category 1 (low-grade adenoma) in ApcMin/+ (left column) and ApcMin/+ Mieap-/- (second from left column) mice, Category 2 (high-grade adenoma, second from right column) and Category 3 (adenocarcinoma, right column) in the ApcMin/+ Mieap-/- mice. Scale bars: 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep12472), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-Paraffin-NB110-96877-img0015.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x. With anti-microbial. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0019.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: liver tissue . Species: Rat. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green).](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0020.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0022.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Effect of Diltiazem on L444P and N370S/V394L GC Folding and Trafficking.Western blot analysis of untreated and diltiazem-treated L444P GC cells. L444P GC cells were cultured without or with diltiazem at varying concentrations for 7 d before the cells were lysed for SDS-PAGE and Western blot analysis. GC was detected using mouse anti-GC antibody 2E2. Beta-actin served as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pbio.0060026) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Western-Blot-H00002629-M01-img0019.jpg "Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Effect of Diltiazem on L444P and N370S/V394L GC Folding and Trafficking.Western blot analysis of untreated and diltiazem-treated L444P GC cells. L444P GC cells were cultured without or with diltiazem at varying concentrations for 7 d before the cells were lysed for SDS-PAGE and Western blot analysis. GC was detected using mouse anti-GC antibody 2E2. Beta-actin served as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pbio.0060026) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of monoclonal antibody to GBA on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Immunocytochemistry-Immunofluorescence-H00002629-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of monoclonal antibody to GBA on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Immunohistochemistry-Paraffin-H00002629-M01-img0014.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - GBA monoclonal antibody (M01), clone 2E2 Analysis of GBA expression in MCF-7.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Western-Blot-H00002629-M01-img0016.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of GBA expression in transfected 293T cell line by GBA monoclonal antibody (M01), clone 2E2.Lane 1: GBA transfected lysate(60 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Western-Blot-H00002629-M01-img0017.jpg)
![ELISA: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Detection limit for recombinant GST tagged GBA is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-ELISA-H00002629-M01-img0018.jpg)
![Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024] - Specific positive staining can be observed in a subpopulation of mouse gastric mucosa cells. Green is FNDC5. Image from verified customer review.](http://images.novusbio.com/fullsize/Irisin-FNDC5-Antibody-Immunohistochemistry-Paraffin-NBP2-14024-img0011.jpg "Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024] - Specific positive staining can be observed in a subpopulation of mouse gastric mucosa cells. Green is FNDC5. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024] - Staining of human skeletal muscle shows granular cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Irisin-FNDC5-Antibody-Immunohistochemistry-Paraffin-NBP2-14024-img0007.jpg)
![Western Blot: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of Irisin/FNDC5 expression levels detected by Western blot in Normal Human Keratinocytes (HaCat) and Larynx Epidermoid Carcinoma 2 (HEp-2) (A). The mean value of the densitometric optical analysis of the 3 repeats of Western blot detecting irisin expression levels (B). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35053200/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-2552023151496.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415291921.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Positive immunohistochemical (IHC) reactions (brown color) indicating strong irisin expression in the apical parts of cancer cells & vesicles; magnification x400. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415363743.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415384153.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415392544.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415384123.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415384172.jpg)
![Western Blot: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression levels detected by Western blot in Normal Human Keratinocytes (HaCat) & Larynx Epidermoid Carcinoma 2 (HEp-2) (A). The mean value of the densitometric optical analysis of the 3 repeats of Western blot detecting irisin expression levels (B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35053200), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415171334.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-31020241530429.jpg)
![Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Western-Blot-NBP1-30027-img0013.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.")
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Angiotensinogen at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30027-img0014.jpg)
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody - BSA Free [NBP1-30027] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of Serpin A8 (NBP1-30027) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-30027-img0015.jpg)
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Immunohistochemistry-Paraffin-NBP1-30027-img0012.jpg)
![Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032] - IHC-Fr of normal feline retina with antibodies ABCA4 3F4 (red) and HCAR (green). ABCA4 antibody (1:1000) labels outer segments of photoreceptors and HCAR antibody labels cones. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-Frozen-NBP1-30032-img0006.jpg "Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032] - IHC-Fr of normal feline retina with antibodies ABCA4 3F4 (red) and HCAR (green). ABCA4 antibody (1:1000) labels outer segments of photoreceptors and HCAR antibody labels cones. IHC-Fr image submitted by a verified customer review.")
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - staining of adult mouse retina showing specific immunolabeling of the ABCA4 protein. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0001.jpg)
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in wild-type (ABCA4+/+) retina. PNA labels cone photoreceptors. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0003.jpg)
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in heterozygous (ABCA4+/-) retina. PNA labels cone photoreceptors. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ABCA4 Antibody (3F4) [NBP1-30032] - Characterization of ABCA4 mRNA expression and western blot analyses of ABCA4 protein levels in the canine retina. Western blot analyses of ABCA4 (above), GAPDH (middle), and RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Western-Blot-NBP1-30032-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in affected (ABCA4-/-) retina. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0005.jpg)
![Western Blot: ABCA4 Antibody (3F4) [NBP1-30032] - Characterization of ABCA4 mRNA expression & western blot analyses of ABCA4 protein levels in the canine retina.(A) Relative ABCA4 mRNA expression levels by quantitative RT-PCR in three different regions in three dogs with different genotypes (ABCA4+/+, ABCA4+/-, & ABCA4-/-), normalized to GAPDH expression. (B) Western blot analyses of ABCA4 (above), GAPDH (middle), & RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-310202415371970.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-3102024161606.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-31020241616064.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-31020241616097.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-310202416175238.jpg)
![Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.](http://images.novusbio.com/fullsize/HSD3B1-Antibody-FDO66Q-Immunohistochemistry-NB110-78644-img0002.jpg "Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.")
![Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP2-22166-img0006.jpg "Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - 293T cells transfected with IDH2-flag expression construct were fixed and stained with anti-IDH2 antibody followed by Alexa Fluor 488 anti-rabbit secondary antibody. DAPI staining shows nuclei. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22166-img0007.jpg)
![Immunohistochemistry: IDH2 Antibody [NBP2-22166] - Staining of IDH2 in mouse intestine.](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunohistochemistry-NBP2-22166-img0003.jpg)
![Western Blot: IDH2 Antibody [NBP2-22166] - Analysis of Caki-1 cell lysate (A), human skeletal muscle tissue (B), mouse skeletal muscle tissue (C), and rat skeletal muscle tissue (D) using IDH2 antibody at a concentration of 2 ug/mL.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP2-22166-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - IDH2 antibody was tested in U2OS cells with DyLight (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22166-img0001.jpg)