| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-164SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-106SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85740-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61576SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-54155SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB200-607-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56564SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-102SS | |||
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody Peroxidase conjugated at 1:1,000 for 30 min at RT Block for30 min RT Predicted/Observed size: 18 kDa, 18 kDa.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0012.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody Peroxidase conjugated at 1:1,000 for 30 min at RT Block for30 min RT Predicted/Observed size: 18 kDa, 18 kDa.")
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Staining of mouse carotid artery. A) No Ab staining of WT uninjured Ms. B) IL-1 beta staining of cells after surgical injury of tissue. C) No Ab staining of injured tissue from an IL-1 beta KO.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-42767-img0004.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis of Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:50 45C RT](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunohistochemistry-NBP1-42767-img0010.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the Biotin conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1beta Recombinant Protein. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0008.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Detects 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1Beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1Beta precursor molecule. Denatured IL-1Beta will have a 18 kDa band.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0011.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Western blot analysis of lipid transporters in macrophages treated with or without oxLDL using antibodies against ABCA1 (NB400-164), SR-B1 (NB400-104), and CD36 (NB400-144) . Lrp1Y63F impairs Abca1-mediated cholesterol efflux and increases lipid accumulation in macrophages. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/29292) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA1-Antibody-3A1-891-3-Western-Blot-NB400-164-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Western blot analysis of lipid transporters in macrophages treated with or without oxLDL using antibodies against ABCA1 (NB400-164), SR-B1 (NB400-104), and CD36 (NB400-144) . Lrp1Y63F impairs Abca1-mediated cholesterol efflux and increases lipid accumulation in macrophages. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/29292) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ABCA1 Antibody (3A1.891.3) [NB400-164] - Analysis of FFPE mouse liver using ABCA1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Plasma membrane staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/ABCA1-Antibody-3A1-891-3-Immunohistochemistry-Paraffin-NB400-164-img0007.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Detection of ABCA1 using NB400-164 at various dilutions in ABCA1 transfected HeLa lysates.](http://images.novusbio.com/fullsize/ABCA1-Antibody-3A1-891-3-Western-Blot-NB400-164-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Analysis of ABCA1 in (A) T09 treated RAW 264.7 cells and (B) untreated RAW 264.7 cells.](http://images.novusbio.com/fullsize/ABCA1-Antibody-3A1-891-3-Western-Blot-NB400-164-img0006.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Lrp1Y63F mutation downregulates hepatic Abca1 levels.(A) Western blot analysis of Abca1 expression in the liver from Lrp1Y63F;Ldlr−/− & Ldlr−/− mice fed with HCHF diet for 16 weeks (n = 5, each group). (B) Western blot analysis of Abca1 expression in the liver from 16 week HCHF-fed Lrp1Y63F;Ldlr−/− & Ldlr−/− mice after BMT (n = 5, each group). All data are mean ±SEM. **p<0.01. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/29292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-164_rat-monoclonal-abca1-antibody-3a1-891-3-31020241535616.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Lrp1Y63F mutation has no effect on atherogenic signaling pathways & morphological phenotype in SMCs.(A) Left: representative images of H&E stained descending thoracic aortas (scale bar = 50 μm). Right: aortic wall thickness & cell number were quantified for the indicated genotypes. Data are expressed mean ±SEM; n = 3 per group & genotype. (B) HCHF diet in Lrp1Y63F;Ldlr−/− mice has no effect on PDGF & TGF beta -mediated signaling in the aorta. Proteins were extracted from whole aorta in Lrp1Y63F;Ldlr−/− & Ldlr−/− mice fed with indicated diets. Expression of proteins involved in PDGF signaling pathways was determined by Western blot analysis. (C) Lrp1Y63F mutation did not affect atherogenic signaling pathways mediated by PDGF in vitro. Primary SMCs from WT & Lrp1Y63F mice were incubated with or without 10 ng/ml PDGF-BB for 10 min. Expression of proteins involved in PDGF signaling were determined by Western blot analysis. (D) Lrp1Y63F mutation did not alter SMC functions in vitro. Primary cultured SMCs from WT & Lrp1Y63F mice were incubated with or without 10 ng/ml PDGF-BB under indicated conditions, then cell migration (left) & proliferation (right) were determined. Values from three independent experiments are expressed as mean ±SEM. *p<0.05. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/29292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-164_rat-monoclonal-abca1-antibody-3a1-891-3-310202416175236.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Lrp1Y63F impairs Abca1 induction through inhibiting the PPAR gamma /LXR pathway.(A) Real-time PCR analysis of Abca1, Abca7, Pparg, Nr1h3 & Nr2h2 expression in macrophages treated with or without ox-LDL. (B–E) Isolated peritoneal macrophages from Lrp1Y63F;Ldlr−/− & Ldlr−/− were pre-incubated for 1 hr with (B) T0070907 (PPAR gamma antagonist, 10 μM), (C) Rosiglitizone (PPAR gamma agonist) at indicated concentrations in μM, (D) T0901317 (nonspecific LXR agonist) at indicated concentrations in μM, (E) LXR623 (LXR beta full agonist & LXR alpha partial agonist) at indicated concentrations in μM, followed by the treatment with or without oxLDL for an additional 24 hr. Abca1 expression was assessed by immunoblot analysis (left). Protein expression was quantified & analyzed (right). Data are mean ±SEM from three independent experiments. *p<0.05; **p<0.01. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/29292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-164_rat-monoclonal-abca1-antibody-3a1-891-3-310202416154639.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Lrp1Y63F mutation has no effect on atherogenic signaling pathways & morphological phenotype in SMCs.(A) Left: representative images of H&E stained descending thoracic aortas (scale bar = 50 μm). Right: aortic wall thickness & cell number were quantified for the indicated genotypes. Data are expressed mean ±SEM; n = 3 per group & genotype. (B) HCHF diet in Lrp1Y63F;Ldlr−/− mice has no effect on PDGF & TGF beta -mediated signaling in the aorta. Proteins were extracted from whole aorta in Lrp1Y63F;Ldlr−/− & Ldlr−/− mice fed with indicated diets. Expression of proteins involved in PDGF signaling pathways was determined by Western blot analysis. (C) Lrp1Y63F mutation did not affect atherogenic signaling pathways mediated by PDGF in vitro. Primary SMCs from WT & Lrp1Y63F mice were incubated with or without 10 ng/ml PDGF-BB for 10 min. Expression of proteins involved in PDGF signaling were determined by Western blot analysis. (D) Lrp1Y63F mutation did not alter SMC functions in vitro. Primary cultured SMCs from WT & Lrp1Y63F mice were incubated with or without 10 ng/ml PDGF-BB under indicated conditions, then cell migration (left) & proliferation (right) were determined. Values from three independent experiments are expressed as mean ±SEM. *p<0.05. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/29292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-164_rat-monoclonal-abca1-antibody-3a1-891-3-31020241616063.jpg)
![Western Blot: PLTP Antibody [NB400-106] - Activation of PLTP in STAT3 pathway. RA-FLS were stimulated for 24 hours with rhPLTP at the indicated concentrations. Western blot analyses analyzed cell lysates for phosphorylation of STAT3 (Tyr705). Band intensities were normalized to corresponding STAT3 band intensities. Representative Western blots are shown (n = 3).Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.](http://images.novusbio.com/fullsize/PLTP-Antibody-Western-Blot-NB400-106-img0006.jpg "Western Blot: PLTP Antibody [NB400-106] - Activation of PLTP in STAT3 pathway. RA-FLS were stimulated for 24 hours with rhPLTP at the indicated concentrations. Western blot analyses analyzed cell lysates for phosphorylation of STAT3 (Tyr705). Band intensities were normalized to corresponding STAT3 band intensities. Representative Western blots are shown (n = 3).Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.")
![Immunocytochemistry/Immunofluorescence: PLTP Antibody [NB400-106] - PLTP antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunocytochemistry-Immunofluorescence-NB400-106-img0002.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP expression and activity in RA and OA joints and 3OH-myristate levels in synovial fluid of RA and OA patients. Immunohistological analysis of PLTP expression in synovial tissue from 5 RA and 6 OA patients stained for PLTP and macrophages (CD68+ cells); All RA synovial tissue showed positive stainings while only 2 out 6 OA tissues were positive for PLTP staining. Representative stainings are shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29565987/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-NB400-106-img0008.jpg)
![Western Blot: PLTP Antibody [NB400-106] - WB analysis of PLTP in human brain lysate.](http://images.novusbio.com/fullsize/PLTP-Antibody-Western-Blot-NB400-106-img0001.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP antibody expression in RA synovial tissues. (A) Immuno-histological analyses used RA patients synovial tissue sections (n=5). They were stained for PLTP or macrophages (CD68+ cells). Blue arrows indicate macrophages (non-exhaustive), determined as CD68+ cells. FLS are determined with CD68+ staining (black arrow; non-exhaustive) and morphological features. (B, C) Double staining displays localization (n = 3) of PLTP with macrophages (CD68+) in B or RA-FLS (alpha-SMA+) in C. Overlay is shown to visualize co-localization of PLTP. All magnification and fluorescence analysis at 20x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29565987/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-NB400-106-img0003.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - Recombinant PLTP antibody induced cytokine production and FLS proliferation, independent of its lipid transfer ability. (A) RA-FLS were stimulated for 48 hours with PLTP or heat inactivated-PLTP and proliferation was evaluated during the final day of stimulation using thymidine incorporation. (B) Blockade of PLTP decreased the effect of rhPLTP on FLS proliferation. (C) PLTP impact on RA-FLS cytokine production. (D) OA-FLS were treated with either native PLTP or heated-PLTP and analyzed for cytokine production and proliferation. Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-NB400-106-img0005.jpg)
![Immunohistochemistry-Paraffin: PLTP Antibody [NB400-106] - PLTP was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human PLTP polyclonal Antibody (Catalog # NB400-106) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in decidual cells.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-Paraffin-NB400-106-img0007.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP expression in RA synovial tissues.For immuno-histological analysis, (A) synovial tissue sections from RA patients (n = 5) were stained for PLTP or macrophages (CD68+ cells). Representative images obtained for immunohistological staining are shown. Blue arrows show macrophages (non-exhaustive), determined as CD68+ cells. FLS are determined with morphological features & CD68+ staining (black arrow; non-exhaustive). (B, C) Double staining was performed to visualize localization (n = 3) of PLTP with macrophages (CD68+) (B) or RA-FLS ( alpha -SMA+) (C). Fluorescence was analyzed at 20x magnification. Overlay is shown to visualize co-localization of PLTP in macrophages (CD68+) (B) or RA-FLS ( alpha -SMA+) (C) or PLTP expression in infiltrate. Original magnification: 20x. Separate images can be found in S1 Fig. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0193815), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-106_rabbit-polyclonal-pltp-antibody-310202416171467.jpg)
![Immunocytochemistry/Immunofluorescence: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining for human aortic valve endothelial cells. Image from a verified customer review.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85740-img0024.jpg "Immunocytochemistry/Immunofluorescence: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining for human aortic valve endothelial cells. Image from a verified customer review.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human liver shows strong membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP1-85740-img0027.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human aortic valve staining for CD73. Image from verified customer review.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-NBP1-85740-img0019.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining for pig aortic valve. Image from verified customer review.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-NBP1-85740-img0020.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human duodenum shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP1-85740-img0025.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP1-85740-img0026.jpg)
![Western Blot: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or Duox2 and analyzed for Nox4 and Duox1/2 proteins in 2 experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0154157), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Knockdown-Validated-NB110-61576-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or Duox2 and analyzed for Nox4 and Duox1/2 proteins in 2 experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0154157), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: DUOX2 Antibody [NB110-61576] - Detection of DUOX2 in A549 in cell lysate.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Western-Blot-NB110-61576-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DUOX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61576-img0004.jpg)
![Western Blot: DUOX2 Antibody [NB110-61576] - Generation and validation of NOX4 knockout (KO) HeLa cell lines. NOX4 knockout did not influence NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 levels. Protein levels of NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 in parental HeLa cells and three clones of NOX4 knockout cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0170327), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Western-Blot-NB110-61576-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - DUOX2 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/DUOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61576-img0003.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Liver tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.](http://images.novusbio.com/fullsize/iNOS-Antibody-K13-A-Immunohistochemistry-Paraffin-NBP1-33780-img0011.jpg "Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Liver tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.")
![Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Lung adenocarcinoma tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.](http://images.novusbio.com/fullsize/iNOS-Antibody-K13-A-Immunohistochemistry-Paraffin-NBP1-33780-img0012.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Inflamed porcine intestine. Antibody dilution 1:200, overnight 4C. Secondary anti-rabbit Alexa 488 (1:1000) 2 hr incubation. Image from verified customer review.](http://images.novusbio.com/fullsize/iNOS-Antibody-K13-A-Immunohistochemistry-Paraffin-NBP1-33780-img0010.jpg)
![Western Blot: iNOS Antibody (K13-A) [NBP1-33780] - Effects of omega-3 supplementation on the immunocontent of macrophages M1 markers: nitric oxide synthase 2 (NOS-2) (A) & CD86 (B), & M2 marker: Arginase-1 (C) on the mouse paw muscle 48 h after the model induction in Saline/Sham, Saline/CPIP, Omega-3/CPIP, & Corn oil/CPIP groups. Data are expressed as mean ± SD of 6 animals per group, statistically assessed by the one-way ANOVA followed by Tukey’s test. ##p < 0.01 vs. the Saline/Sham group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35391753), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33780_rabbit-monoclonal-inos-antibody-k13-a-31020241535639.jpg)
![Western Blot: Lipase A Antibody [NBP1-54155] - Analysis using NBP1-54155 on cellular extracts of human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell lines, and HFL1 Normal primary human fibroblasts. NBP1-54155 revealed a strong specific band at the expected molecular weight for Lipase A at 45 kDa in different lysates of human cancer cell lines. NBP1-54155 specifically reveals LIPA in extracts from human prostate adenoma tissue and prostate cancer cell lines DU145, PC3, and 22RV1.The protein bands were visible in the positive but not in the negative controls. Therefore, NBP1-54155 was found to be suitable for checking the expression levels of Lipase A protein. Image from verified customer review.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: Lipase A Antibody [NBP1-54155] - Analysis using NBP1-54155 on cellular extracts of human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell lines, and HFL1 Normal primary human fibroblasts. NBP1-54155 revealed a strong specific band at the expected molecular weight for Lipase A at 45 kDa in different lysates of human cancer cell lines. NBP1-54155 specifically reveals LIPA in extracts from human prostate adenoma tissue and prostate cancer cell lines DU145, PC3, and 22RV1.The protein bands were visible in the positive but not in the negative controls. Therefore, NBP1-54155 was found to be suitable for checking the expression levels of Lipase A protein. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: Lipase A Antibody [NBP1-54155] - NR and Metf promote FoxO1-mediated Lipa upregulation in visceral AT of adult mice. Adult C57/BL6 mice (5 months) were nutrient restricted (NR) by 24 h fasting or treated for 10 days with Metf (400 mg/kg) dissolved in drinking water (n=4 mice per group). Western blot of FoxO1 and Lipa in total protein extracts of explanted visceral (epididymal) AT. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013404) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0009.jpg)
![Simple Western: Lipase A Antibody [NBP1-54155] - Image shows a specific band for Lipase A in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Simple-Western-NBP1-54155-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Lipase A Antibody [NBP1-54155] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lipase A Antibody NBP1-54155 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54155-img0010.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody - BSA Free [NBP1-54155] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of Lipase A antibody (NBP1-54155). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Lipase-A-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-54155-img0011.jpg)
![Western Blot: Lipase A Antibody [NBP1-54155] - Detection of LIPA in HepG2 whole cell extract.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0001.jpg)
![Immunohistochemistry: Lipase A Antibody [NBP1-54155] - Staining of LIPA in mouse stomach smooth muscle.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-NBP1-54155-img0005.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416154610.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416165629.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - AMPK drives Lipa-released FFAs oxidation restraining energetic catastrophe. (a) 3T3-L1 cells were transfected with DN-AMPK or empty vector. RT-qPCR analysis of relative peroxisome proliferator-activated receptor gamma-1 alpha , peroxisome proliferator-activated receptor-alpha , carnitine palmitoyltransferase 1b & acyl-CoA oxidase 1 mRNA levels were performed after 4 h of NR or 16 h of Metf treatment. Dashed line indicates the mRNA value of untreated DN-AMPK cells (Ctr). mRNA levels of untreated cells transfected with empty vector were similar to untreated DN-AMPK cells (data not shown). (b) Cheminoluminescent assay of ATP level in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector after 8 h NR or 16 h Metf treatment. ATP level was expressed as pmol ATP per mg protein. (c) After 8 h of NR or 16 h Metf treatment, FFAs were enzymatically detected in culture medium of 3T3-L1 adipocytes transfected with DN-AMPK or empty vector. Values were expressed as μg FFAs per mg protein. (d) Left panel: western blot of AMPKpT172, PARP-1 & cleaved form of caspase-3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & subjected to 8 h NR. Right panel: cytofluorimetric analysis of apoptosis in DN-AMPK cells subjected to 8 h NR. (e) Western blot of PARP-1 & cleaved form of caspase-3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & treated with Metf for 16 h. (f) Western blot of FoxO1, Lipa, LC3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & subjected to 4 h NR. beta -actin was used as loading control. All values are given as mean±S.D. *P<0.05, **P<0.01 versus controls; °P<0.05, °°P<0.01 versus Metf treatment. All data are representative of at least three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-31020241618936.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416171493.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416171436.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - Lipa downregulation impairs lipid breakdown & elicits cell death in nutrient restricted adipocytes. (a) 3T3-L1 adipocytes were transfected with siRNA against Lipa (Lipa(−)) or with a scramble siRNA (Scr). Western blot of Lipa, PARP-1 & cleaved form of caspase-3 in total protein extracts from 3T3-L1 adipocytes after 4 h of NR. (b) TG content was quantified by ORO staining in fixed 3T3-L1 adipocytes 6 h after NR. (c) RT-qPCR analysis of relative peroxisome proliferator-activated receptor gamma-1 alpha , peroxisome proliferator-activated receptor-alpha & carnitine palmitoyltransferase 1b mRNA levels was performed in 3T3-L1 adipocytes 4 h after NR. (d) FFAs were analyzed in culture medium 6 h after NR. beta -actin was used as loading control. All values are given as mean±S.D. *P<0.05, **P<0.01 versus controls; °P<0.05 versus NR treatment. All data are representative of at least three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416163713.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-31020241616083.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Differential expression of CAIX and CAXII in patient-derived tumor grafts. Frozen tissue samples from PDX tumors were homogenized in RIPA buffer containing protease inhibitor. Western blots were probed with antibodies for CAIX, CAXII, CAII, ER, E-cadherin, actin, and GAPDH. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0199476) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0008.jpg "Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Differential expression of CAIX and CAXII in patient-derived tumor grafts. Frozen tissue samples from PDX tumors were homogenized in RIPA buffer containing protease inhibitor. Western blots were probed with antibodies for CAIX, CAXII, CAII, ER, E-cadherin, actin, and GAPDH. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0199476) licensed under a CC-BY license.")
![Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Staining in nucleus and cytoplasm of proximal and distal tubules, bowman's capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 ug/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3'3-diamino-benzidine substrate (DAB) or WARP RED.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Immunohistochemistry-NB600-919-img0005.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Detection of Carbonic Anhydrase II in mouse embryonic whole cell lysate. Image provided via product review by verified customer.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0001.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - analysis of Carbonic Anhydrase II protein (loading amount: 50 ng) using rabbit anti-Carbonic Anhydrase II antibody (NB600-919) at a dilution of 1:1000, incubation - overnight at 4C.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0002.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Analysis using the Biotin conjugate of NB600-919. Detection of Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic Anhydrase II Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptav](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0004.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II. Other band(s): None.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0007.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Localization of CAII in mouse peritoneum. Image from verified customer review.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Immunohistochemistry-Paraffin-NB600-919-img0003.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NB100-59740] - (0.003ug/ml) staining of Mouse (A) and Rat (B) Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-59740_goat-polyclonal-arginase-1-arg1-liver-arginase-antibody-1652023918335.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NB100-59740] - (0.003ug/ml) staining of Mouse (A) and Rat (B) Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: Caspase-1 Antibody [NB100-56564] - Human epidermoid carcinoma parental cell line and Caspase-1 knockout (KO) A431 cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # NB100-56564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Knockout-Validated-NB100-56564-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Caspase-1 Antibody [NB100-56564] - Human epidermoid carcinoma parental cell line and Caspase-1 knockout (KO) A431 cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # NB100-56564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Caspase-1 Antibody [NB100-56564] - Analysis of Caspase-1 using a polyclonal antibody. Human HeLa lysate probed with Caspase-1 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Western-Blot-NB100-56564-img0005.jpg)
![Immunohistochemistry-Paraffin: Caspase-1 Antibody [NB100-56564] - Human reactive lymph node stained for Caspase-1 expression (NB100-56564) using a DAB chromogen and Hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Immunohistochemistry-Paraffin-NB100-56564-img0004.jpg)
![Western Blot: COX-2 Antibody [NB100-868] - A549 (A) and Daudi (B) cell lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COX-2-Antibody-Western-Blot-NB100-868-img0007.jpg "Western Blot: COX-2 Antibody [NB100-868] - A549 (A) and Daudi (B) cell lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: COX-2 Antibody [NB100-868] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15%Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasm and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/COX-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-868-img0006.jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-868] - 10x image of mouse lung tissue expressing cox-2. Secondary antibody: Alexa Fluor 546 (red), counterstain: DAPI (blue). Goat anti-mouse cox-2 immunofluorescence staining of paraffin embedded fixed mouse lung tissue sections. Antigen retrieval in citrate buffer (95C, 20 min) followed by permeabilization with 0.2% Triton X-100 for 20 min. Sections were blocked in 5% donkey serum for 1 hr at RT, and incubated with NB100-868 1:100 dilution in 3% donkey serum overnight at 4 C. Incubated with secondary donkey anti-goat AF546 at 1:500 dilution 1 hr at RT. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/COX-2-Antibody-Immunohistochemistry-Paraffin-NB100-868-img0008.jpg)
![Flow Cytometry: COX-2 Antibody [NB100-868] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line)](http://images.novusbio.com/fullsize/COX-2-Antibody-Flow-Cytometry-NB100-868-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: COX-2 Antibody [NB100-868] - Paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml, showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue)](http://images.novusbio.com/fullsize/COX-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-868-img0005.jpg)
![Western Blot: SR-BII Antibody [NB400-102] - Western blot analysis of SR-BII in 1. Human heart lysate 2. Mouse heart lysate](http://images.novusbio.com/fullsize/SR-BII-Antibody-Western-Blot-NB400-102-img0002.jpg "Western Blot: SR-BII Antibody [NB400-102] - Western blot analysis of SR-BII in 1. Human heart lysate 2. Mouse heart lysate")
![Immunocytochemistry/Immunofluorescence: SR-BII Antibody [NB400-102] - SR-BII antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/SR-BII-Antibody-Immunocytochemistry-Immunofluorescence-NB400-102-img0003.jpg)